Anti p44 42 mapk erk1 2 9102s

Manufactured by Cell Signaling Technology

Anti-p44/42 MAPK (Erk1/2) (9102S) is a primary antibody produced by Cell Signaling Technology. It recognizes the extracellular signal-regulated kinases 1 and 2 (Erk1/2), which are members of the mitogen-activated protein kinase (MAPK) family.

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Lab products found in correlation

Anti pten 138g6, by Cell Signaling Technology (1 mentions) Rabbit polyclonal anti gapdh 14c10, by Cell Signaling Technology (1 mentions) Protease and phosphatase inhibitor cocktail, by Roche (1 mentions) Anti s6 ribosomal protein, by Cell Signaling Technology (1 mentions) Anti mouse 170 6516, by Bio-Rad (1 mentions) Antirabbit 170 6515, by Bio-Rad (1 mentions)

2 protocols using anti p44 42 mapk erk1 2 9102s

Western Blot Analysis of PTEN, Akt, and MAPK

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For Western blot, cell and tissue lysates were prepared with 150-RIPA buffer and protease and phosphatase inhibitor cocktails (Roche). The following antibodies were used for Western blotting: rabbit polyclonal anti-PTEN (138G6; 1:1000, Cell Signaling Technology), rabbit polyclonal anti-phospho-Akt(S473) (9271S; 1:1000, Cell Signaling Technology), rabbit polyclonal anti-Akt (9272S; 1:1000, Cell Signaling Technology), rabbit polyclonal anti-GAPDH (14C10; 1:6000, Cell Signaling Technology), rabbit polyclonal anti-p44/42 MAPK (Erk1/2) (9102S; 1:3000, Cell Signaling), rabbit polyclonal anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (9101S; 1:3000, Cell Signaling).

Lunardi A., Webster K.A., Papa A., Padmani B., Clohessy J.G., Bronson R.T, & Pandolfi P.P. (2014). Role of aberrant PI3K pathway activation in gallbladder tumorigenesis. Oncotarget, 5(4), 894-900.

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Immunoblotting Analysis of Protein Signaling

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Proteins were extracted and visualized as reported in [36 (link)]. Immunoblotting was carried out with the following antibodies: anti-phospho-p44/42 MAPK (ERK 1/2) (Thr202/Tyr204) #9101S, anti-p44/42 MAPK (ERK1/2) #9102S, anti-phospho-S6 Ribosomal Protein (Ser235/236) #221 and anti-S6 Ribosomal protein #2217 purchased from Cell Signaling Technology. Anti-RAN #sc-271376 was purchased from Santa Cruz Biotechnology. The secondary antibodies anti-rabbit #170–6515 and anti-mouse #170–6516 from Bio Rad were used.

Ndembe G., Intini I., Moro M., Grasselli C., Panfili A., Panini N., Bleve A., Occhipinti M., Borzi C., Garassino M.C., Marabese M., Canesi S., Scanziani E., Sozzi G., Broggini M, & Ganzinelli M. (2024). Caloric restriction and metformin selectively improved LKB1-mutated NSCLC tumor response to chemo- and chemo-immunotherapy. Journal of Experimental & Clinical Cancer Research : CR, 43, 6.

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