Human umbilical cord endothelial cells huvec

All Adv5 mutants used in the present studies were constructed in in-house laboratories following protocols detailed elsewhere. Ad5/dE1A with deletion of amino acids 121–129 in E1A CR2 was previously constructed[36] (link). M7 and M8 were derived from Ad5/dE1A through replacement of the ADP and gp19k open reading frame in the E3 region by a fragment of HSV-TK cDNA, respectively. Ad5/dE1A/dgp19k and Ad5/dE1A/dADP were derived from Ad5/dE1A through deletion of the gp19k and ADP open reading frame and used as a vector control for M7 and M8, respectively. The replication-deficient adenovirus vector Adv-TK, containing HSV-TK gene under control of a Rous sarcoma virus long terminal repeat promoter in the region of the excised E1 adenoviral genes, was constructed in the in house laboratories as previously described[37] (link). Wt-Ad5 and the following cell lines A549, HepG2, MCF-10A (Non-transformed mammary epithelial cell lines) and 293 cell lines were purchased from the American Type Culture Collection (Manassas, VA), A2780, MKN-45 was obtained from the China Center for Type Culture Collection (Shanghai, P.R. China). Human umbilical cord endothelial cells (HUVEC) were purchased from Lonza (Allendale, NJ) and cultured following the manufacturer’s instructions.