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Cd4 cd25 t regulatory t cell isolation kit

Manufactured by Miltenyi Biotec
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The CD4+CD25+ T regulatory T cell isolation kit is a laboratory tool designed to isolate CD4+CD25+ regulatory T cells from biological samples. It utilizes a magnetic bead-based separation system to selectively enrich for these cell types, which are important in immune regulation and research applications.

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Lab products found in correlation

Clone 145 2c11, by BD (2 mentions) Clone 37, by BD (2 mentions) Soluble αcd28, by BD (2 mentions) α mouse cd3, by BD (2 mentions)

Close spelling variants of this product

CD4+CD25+ Regulatory T Cells Isolation Kit CD4+CD25+CD127dim/– Regulatory T Cells Isolation Kit II CD4+CD25+ isolation kits Cell isolation kits Isolation kits

2 protocols using cd4 cd25 t regulatory t cell isolation kit

1

Enrichment of CD4+CD25+ Regulatory T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell suspensions were prepared from MLNs and spleens as previously described46 (link). MACS-enriched CD4+CD25+ T cells (CD4+CD25+ T regulatory T cell isolation kit; Miltenyi Biotec), were cultured in 96-well plates coated with α–mouse CD3 (1 µg/ml, clone 145-2C11) and soluble α-CD28 (0.5 µg/ml, clone 37.51) (both BD Bioscience). PPT and DPN were added at 10 nM (Tocris Bioscience).
Goodman W.A., Garg R.R., Reuter B.K., Mattioli B., Rissman E.F, & Pizarro T.T. (2014). Loss of estrogen-mediated immunoprotection underlies female gender bias in experimental Crohn’s-like ileitis. Mucosal immunology, 7(5), 1255-1265.
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2

Enrichment of CD4+CD25+ Regulatory T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell suspensions were prepared from MLNs and spleens as previously described46 (link). MACS-enriched CD4+CD25+ T cells (CD4+CD25+ T regulatory T cell isolation kit; Miltenyi Biotec), were cultured in 96-well plates coated with α–mouse CD3 (1 µg/ml, clone 145-2C11) and soluble α-CD28 (0.5 µg/ml, clone 37.51) (both BD Bioscience). PPT and DPN were added at 10 nM (Tocris Bioscience).
Goodman W.A., Garg R.R., Reuter B.K., Mattioli B., Rissman E.F, & Pizarro T.T. (2014). Loss of estrogen-mediated immunoprotection underlies female gender bias in experimental Crohn’s-like ileitis. Mucosal immunology, 7(5), 1255-1265.
+ Open protocol
+ Expand

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