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Vectasheild mounting medium with dapi

Manufactured by Vector Laboratories
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Vectashield Mounting Medium with DAPI is a ready-to-use aqueous mounting medium designed for use with fluorescence microscopy. It contains the nuclear counterstain DAPI, which binds to DNA and emits blue fluorescence when excited.

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Lab products found in correlation

Tunel system, by Promega (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Deadend fluorometric terminal, by Promega (1 mentions) Alexa fluor 633 phalloidin, by Thermo Fisher Scientific (1 mentions) Syto62, by Thermo Fisher Scientific (1 mentions) 510 meta laser scanning confocal microscope, by Zeiss (1 mentions) Texas red streptavidin, by Vector Laboratories (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Bioprime dna labeling system, by Thermo Fisher Scientific (1 mentions) Dig nick translation mix, by Roche (1 mentions)

Close spelling variants of this product

Mounting medium (280 citations) DAPI mounting medium (92 citations) Mounting Medium with DAPI (37 citations) Vectasheild (7 citations) Vectasheild with DAPI (6 citations) VECTASHEILD mounting medium (5 citations) VECTASHEILD antifade mounting medium with DAPI (2 citations)

4 protocols using vectasheild mounting medium with dapi

1

Cell Viability and DNA Fragmentation

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Cell viability was determined by using the CellTiter-Glo luminescent cell viability assay (Promega Corporation, Madison, WI, USA) according to the manufacturer’s directions. DNA fragmentation was determined by using the DeadEnd™ Fluorometric terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) System (Promega Corporation) according to the manufacturer’s directions. Nuclei were stained using Vectasheild mounting medium with DAPI (Vector Laboratories, Inc., Burlingame, CA, USA).
Kim J.E., Kim H., An S.S., Maeng E.H., Kim M.K, & Song Y.J. (2014). In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells. International Journal of Nanomedicine, 9(Suppl 2), 235-241.
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2

Immunofluorescence Staining of H. pylori Infection

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AGS or GSM06 cells were grown to 70% confluency on glass cover slips in the wells of 24 well cluster plates. Following infection with H. pylori the cells were washed in PBS, fixed in methanol at –20°C, and blocked in 1% normal goat serum. Cells were stained using sera from mice immunized with HpSS1 lysate antigen as the primary antibody, followed by goat anti-mouse FITC conjugate. The cells were counter-stained with AlexaFluor 633 phalloidin (Molecular Probes, Inc., Eugene, OR), mounted in Vectasheild mounting medium with DAPI (Vector Laboratories, Inc., Burlingame,CA), and examined for fluorescence by confocal microscopy using Leica Confocal Software.
Banerjee A., Basu M., Blanchard T.G., Chintalacharuvu S.R., Guang W., Lillehoj E.P, & Czinn S.J. (2016). Early molecular events in murine gastric epithelial cells mediated by Helicobacter pylori CagA. Helicobacter, 21(5), 395-404.
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3

In situ Imaging of Oxidative Stress

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In situ staining of superoxide (O2•−) and H2O2 levels were determined using the superoxide indicator dihydroethidium dihydroethidium (DHE) and the ROS indicator 5-(6)-chloromethyl-20,70-dichlorodihydrofluorescein diacetate (CM-H2DCFDA). M.abs bacteria were labeled with Syto-62 according to manufacturer's instruction (Invitrogen, Grand Island, NY). TPA-stimulated THP-1 cells were grown on a glass chamber slide and were infected with Syto-62-labeled M.abs for 1 h, and incubated with media for 4 h at CO2 incubator. Thirty minutes before the infection was complete, DHE, and DCF were added to the assigned chambers. After infection was complete, the medium was removed, and chambers were washed, and mounted with Vectasheild mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Images were viewed using Zeiss 510 Meta Confocal Laser Scanning Microscope.
Abdalla M.Y., Ahmad I.M., Switzer B, & Britigan B.E. (2015). Induction of heme oxygenase-1 contributes to survival of Mycobacterium abscessus in human macrophages-like THP-1 cells. Redox Biology, 4, 328-339.
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4

Metaphase Spread Analysis of T and B Cells

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B and T cells stimulated for 48 hours were arrested in metaphase by incubating with 0.0μg/mL colcemid (KaryoMax) and 0.45mM BrdU (Sigma) for 2 hours. Metaphase arrested cells were isolated by hypotonic treatment (40mM KCl, 0.5mM EDTA, 20mM HEPES, pH7.4) and fixation in methanol:acetic acid (3:1 volume). The fixed cells were then dropped on slides at 4°C and dried at 75°C for 5 minutes. Metaphase spreads were hybridized overnight with relevant Tcrb and Igh bacterial artificial chromosome (BAC) probes: Vβ-DβJβ1, RP23-203H5; Cβ, 164G11; VH-DH, RP24-275L15, and 3'IgH, CT7-199M11. Cβ and 3'IgH probes were labeled using the DIG-NICK Translation Mix (Roche). Vβ-DβJβ1 and VH-DH probes were labeled using the BioPrime DNA Labeling System (Invitrogen). Probes were detected using Fitc-anti-digoxin Fab (Roche) and Texas red-streptavidin (Vector Laboratories). Coverslips were mounted with Vectasheild mounting medium with DAPI (Vector). Images were captured and analyzed using Case Data Manager (Applied Spectral Imaging).
Steinel N.C., Fisher M.R., Yang-Iott K.S, & Bassing C.H. (2014). The Ataxia Telangiectasia Mutated and Cyclin D3 Proteins Cooperate to Help Enforce TCRβ and IgH Allelic Exclusion. Journal of immunology (Baltimore, Md. : 1950), 193(6), 2881-2890.
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