Ccl11 eotaxin

Manufactured by R&D Systems

Sourced in United States

CCL11/eotaxin is a chemokine that plays a role in the recruitment and activation of eosinophils. It is a member of the CC chemokine family.

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Close spelling variants of this product

CCL11/eotaxin-1 Mouse CCL11/Eotaxin Quantikine ELISA Kit CCL11 Eotaxin

2 protocols using ccl11 eotaxin

Cytokine and Chemokine Profiling in Lung Homogenates

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We removed the whole lungs (5–8 animals per group) immediately following BAL and
prepared lung homogenate supernatants as previously described.^{19 (link)} We converted 1 g of the homogenate suspension to 1 mL and calculated the
protein levels of various cytokines and chemokines in the total lung
homogenates. The levels of interleukin (IL)-4, IL-5, interferon gamma (IFN-γ),
IL-12 (Thermo Fisher Scientific, Inc., Chicago, IL, USA), IL-10, IL-13, IL-33,
macrophage-inflammatory protein 1-apha (MIP-1α), keratinocyte chemoattractant
(KC), monocyte chemoattractant protein-1 (MCP-1), chemokine (C-C motif) ligand
5/regulated and normal T-cell expressed and secreted (CCL5/RANTES),
CCL11/eotaxin (R&D Systems, Minneapolis, MN, USA), and monocyte-chemotactic
protein 3 (MCP-3; PeproTech, Inc., NJ, USA) in the lung homogenate supernatants
were measured using an ELISA kit, according to the manufacturer’s instructions.
Cytokine and chemokine content were expressed as pg in the total lung homogenate
supernatants.

Koike E., Yanagisawa R., Win-Shwe T.T, & Takano H. (2018). Exposure to low-dose bisphenol A during the juvenile period of development disrupts the immune system and aggravates allergic airway inflammation in mice. International Journal of Immunopathology and Pharmacology, 32, 2058738418774897.

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Cytokine and Chemokine Profiling in Infected Rats

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Commercial ELISA kits were used to determine IL-4, IL-10, IFN-γ, TNF-α (BD Biosciences, San Jose, CA, USA) and CCL11/eotaxin (R&D Systems, Minneapolis, MN, USA) levels in intestinal tissue extracts obtained from infected and non-infected rats. Samples were run in duplicate and cytokine or chemokine concentrations were obtained by extrapolation in calibration curves. Results were expressed in pg/ml or ng/ml ± SD.
The levels of IL-5, IL-12 and IL-13 were determined by an immunoelectrotransfer blot assay as previously described [25 (link)]. Membranes were analysed by densitometry using the Image J software (NIH, Bethesda, MD, USA), and the cytokine levels were expressed as mean arbitrary units ± SD and relativized to the α-tubulin band. All reactions were performed in duplicate using ITEs obtained from two independent groups of rats (n = 5 at each dpi studied).

Saracino M.P., Vila C.C., Cohen M., Gentilini M.V., Falduto G.H., Calcagno M.A., Roux E., Venturiello S.M, & Malchiodi E.L. (2020). Cellular and molecular changes and immune response in the intestinal mucosa during Trichinella spiralis early infection in rats. Parasites & Vectors, 13, 505.

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