In all studies, C57BL/6 male and female mice (Jackson Laboratory, Bar Harbor, ME, USA) were given water and chow ad libitum. High fat diet (HFD, 60% of calories from fat, TD.09766) and an isocaloric low fat diet (16.8% of calories from fat, TD.08810) were purchased from Envigo, Inc. (Indianapolis, IN, USA). For the HFD studies, six 6-week-old male mice were placed in each group and maintained on the diets for a total of 18 weeks. At 18 weeks colons were isolated, snap-frozen, and homogenized for analysis. All animal procedures were approved by the Ralph H Johnson VA Medical Center and Medical University of South Carolina Institutional Animal Care and Use Committees (IACUC) and all studies conducted were based on NIH and the American Veterinary Medical Association guidelines.
Td 09766
Manufactured by Inotiv
Sourced in United States
The TD.09766 is a laboratory equipment product. It serves a core function, but detailed information about its intended use or capabilities is not available.
Automatically generated - may contain errors
2 protocols using td 09766
1
High-Fat Diet Induces Colon Changes
2
Genetic Manipulation of Intestinal Cells
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Mice were housed in ventilated cages on a 12-hour light/12-hour dark cycle with corncob bedding and ad libitum access to rodent chow (PicoLab Rodent Diet 20, LabDiet) and water unless otherwise noted. Rbm47fl/fl and Rbm47-IKO lines were generated on a C57BL/6J background, as described previously (7 (link)). The studies used constitutive Vil-Cre [B6.Cg-Tg(Vil1-cre)1000Gum/J], The Jackson Laboratory 021504. We also used the tamoxifen-inducible Vil-Cre [B6.Cg-Tg(Vil1-cre/ERT2)23Syr/J], The Jackson Laboratory 020282 (47 (link)), to generate inducible Rbm47-IKO mice. Because inducible and constitutive Rbm47-IKO mice exhibited similar phenotypes at baseline, further experiments were done only on the constitutive Rbm47-IKO line. Studies show that the Vil1Cre/1000 transgene is expressed extensively in small and large intestine, with only scattered expression in the urogenital tract (48 (link)). Studies were performed on chow-fed mice in the range of 8–14 weeks or where indicated in about 12-month-old mice. Where indicated, mice were fed a high–milk fat diet (TD.09766, Envigo Teklad) for 6 months prior to study. Mice received 100 mg/kg BrdU (MilliporeSigma) 2 hours before harvest. Rbm47fl/fl mice were used as controls in all experiments.
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