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Superscript 3 first strand synthesis master mix

Manufactured by Thermo Fisher Scientific
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SuperScript III First-Strand Synthesis Master Mix is a ready-to-use solution for reverse transcription of RNA into cDNA. It contains the SuperScript III reverse transcriptase enzyme, necessary buffers, and dNTPs.

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Lab products found in correlation

Sybr green pcr master mix, by Thermo Fisher Scientific (2 mentions) Dnase 1, by Thermo Fisher Scientific (2 mentions) Sybr green 1 dye, by Roche (1 mentions) Lightcycler 96, by Roche (1 mentions) 7500 real time pcr system, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

SuperScript III First-Strand Synthesis Mix SuperScript III First-Strand Synthesis SuperScript III First-Strand First Strand Master Mix Superscript III first SuperScript III Synthesis Superscript III

2 protocols using superscript 3 first strand synthesis master mix

1

Quantitative RT-PCR for Gene Expression

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SuperScript III First-Strand Synthesis Master Mix (Invitrogen) was used on 1 µg of RNA to synthesize cDNA. qPCR was performed using SYBR Green I dye (Roche), cDNA, and gene-specific primers. Assays were run in a Light Cycler 96 or 480 (Roche) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using the 2−ΔΔCT method. Each experiment was repeated at least twice, and data were normalized to vehicle controls.
Johnson G.S., Li J., Beaver L.M., Dashwood W.M., Sun D., Rajendran P., Williams D.E., Ho E, & Dashwood R.H. (2017). A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a co-activator of NQO1 in sulforaphane-treated colon cancer cells. Molecular nutrition & food research, 61(4), 10.1002/mnfr.201600769.
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2

RNA Quantification via RT-qPCR

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For each cell culture, 1.0 μg of the extracted RNA was treated with DNase I (18068015, Thermo Fisher) and isolated for reverse transcription by SuperScript™ III First-Strand Synthesis Master Mix (11752050, Invitrogen). RT-qPCR was then applied by using the SYBR™ Green PCR Master Mix (436870, Thermo Fisher). The reactions were performed using an Applied Biosystems® 7500 Real-Time PCR System. The primer sequences are listed in Supplementary Table S3.
Dong W., Oya E., Zahedi Y., Prasad P., Svensson J.P., Lennartsson A., Ekwall K, & Durand-Dubief M. (2020). Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin. Scientific Reports, 10, 6055.
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