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Phosphorylated lkb1

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Phosphorylated-LKB1 is a recombinant protein product that represents the phosphorylated form of the LKB1 (Liver Kinase B1) protein. LKB1 is a serine/threonine kinase that plays a key role in the regulation of cellular energy homeostasis and metabolism.

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Lab products found in correlation

Primary rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions) Phosphorylated ampk, by Cell Signaling Technology (1 mentions) Oridonin c20h28o6, by Merck Group (1 mentions) E cadherin e ca, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Srebp 1c, by Abcam (1 mentions) Phosphorylated ampk, by Abcam (1 mentions) Goat anti rabbit secondary antibody, by Proteintech (1 mentions) Srebp2, by Abcam (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

2 protocols using phosphorylated lkb1

1

Oridonin and AMPK Pathway Regulation

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Oridonin (C20H28O6) and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primary rabbit monoclonal antibodies (diluted at 1:1,000) against phosphorylated-LKB1, phosphorylated- AMPK, E-cadherin (E-Ca), N-cadherin(N-Ca) and β-actin were purchased from Cell Signaling Technology, Inc (Beverly, MA, USA). AICAR and Compound C, OE-LKB1
Kou B., Shi Y., Zhou Z., Yun Y., Wu Q., Zhou J, & Liu W. (2024). Oridonin inhibited epithelial-mesenchymal transition of laryngeal carcinoma by positively regulating LKB1/AMPK signaling. International Journal of Medical Sciences, 21(4), 623-632.
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2

Quantitative Analysis of Cholesterol Proteins

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Total RNA was isolated from liver tissue or cultured cells using the RNeasy mini‐kit (QIAGEN, Maryland) and reverse‐transcribed into complementary DNA for quantitative real‐time PCR assay as described previously.(22)To visualize the expressions of cholesterol‐related proteins, western blotting was used, and the primary antibodies against LDLR (diluted 1:1000; Abcam, Cambridge, United Kingdom), SREBP2 (diluted 1:2,000; Abcam), AMPK (diluted 1:1,000), phosphorylated AMPK (diluted 1:1,000; Abcam), ALR (diluted 1:1,000; Proteintech, Chicago, IL), phosphorylated LKB1 (diluted 1:500, Cell Signaling Technology, Beverly, MA), LKB1 (diluted 1:800, Abcam), SREBP1c (diluted 1:1,000; Abcam), and goat anti‐rabbit secondary antibody (diluted 1:5,000; Proteintech) were used.
Wang X., Dong L., Gai Q., Ai W., Wu Y., Xiao W., Zhang J, & An W. (2020). Lack of Augmenter of Liver Regeneration Disrupts Cholesterol Homeostasis of Liver in Mice by Inhibiting the AMPK Pathway. Hepatology Communications, 4(8), 1149-1167.
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