Log In Sign up
The largest database of trusted experimental protocols

Fish gelatin

Manufactured by Boston BioProducts
Visit Supplier

Fish gelatin is a natural protein-based hydrocolloid derived from the skin, bones, and connective tissues of fish. It is a versatile ingredient used in a variety of applications, including food, pharmaceuticals, and cosmetics.

Automatically generated - may contain errors

Lab products found in correlation

Neutravidin beads, by Thermo Fisher Scientific (3 mentions) Freshly reconstituted guinea pig complement, by Cedarlane (3 mentions) Lsr 2 flow cytometer, by BD (3 mentions) Anti guinea pig c3 antibody conjugated to fitc, by MP Biomedicals (1 mentions) Recombinant ebov gp, by IBT Bioservices (1 mentions)

Close spelling variants of this product

Gelatin

3 protocols using fish gelatin

1

Zika Virus Antibody Complement Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant soluble ZIKV E protein was biotinylated and coupled to red fluorescent Neutravidin beads (Life Technologies). Antibodies were diluted to 5 μg/mL in RPMI-1640, and incubated with GP-coated beads for 2 hrs at 37°C. Freshly reconstituted guinea pig complement (Cedarlane Labs) was diluted in veronal buffer with 0.1% fish gelatin (Boston Bioproducts), added to the antibody-bead complexes, and incubated for 20 min at 37°C. Beads were washed twice with PBS containing 15 mM EDTA, and stained with 1:100 dilution of an anti-guinea pig C3 antibody conjugated to Fluorescein isothiocyanate (FITC; MP Biomedicals) for 15 min at ambient temperature. Beads were washed twice more with PBS, and C3 deposition onto beads was analyzed on a BD LSRII flow cytometer and the MFI of the FITC+ of all beads was measured. Z-score values were calculated as described above.
Gilchuk P., Bombardi R.G., Erasmus J.H., Tan Q., Nargi R., Soto C., Abbink P., Suscovich T.J., Durnell L.A., Khandhar A., Archer J., Liang J., Fouch M.E., Davidson E., Doranz B.J., Jones T., Larson E., Ertel S., Granger B., Fuerte-Stone J., Roy V., Broge T., Linnekin T.C., Linde C.H., Gorman M.J., Nkolola J., Alter G., Reed S.G., Barouch D.H., Diamond M.S., Crowe JE J.r., Van Hoeven N., Thackray L, & Carnahan R. (2020). Integrated pipeline for the accelerated discovery of antiviral antibody therapeutics. Nature biomedical engineering, 4(11), 1030-1043.
+ Open protocol
+ Expand
2

EBOV GP-Mediated Complement Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant EBOV GP (IBT Bioservices) was biotinylated and coupled to red fluorescent Neutravidin beads (Life Technologies). Antibodies were diluted to 5μg/mL in RPMI-1640, and incubated with GP-coated beads for 2 hr at 37°C. Freshly reconstituted guinea pig complement (Cedarlane Labs) was diluted in veronal buffer with 0.1% fish gelatin (Boston Bioproducts), added to the antibody-bead complexes, and incubated for 20 min at 37°C. Beads were washed twice with phosphate buffered saline containing 15 mM EDTA, and stained with an anti-guinea pig C3 antibody conjugated to FITC (MP Biomedicals) for 15 min at ambient temperature. Beads were washed twice more with PBS, and C3 deposition onto beads was analyzed on a BD LSRII flow cytometer and the median fluorescence intensity of the FITC+ of all beads was measured.
Gilchuk P., Kuzmina N., Ilinykh P.A., Huang K., Gunn B.M., Bryan A., Davidson E., Doranz B.J., Turner H.L., Fusco M.L., Bramble M.S., Hoff N.A., Binshtein E., Kose N., Flyak A.I., Flinko R., Orlandi C., Carnahan R., Parrish E.H., Sevy A.M., Bombardi R.G., Singh P.K., Mukadi P., Muyembe-Tamfum J.J., Ohi M.D., Saphire E.O., Lewis G.K., Alter G., Ward A.B., Rimoin A.W., Bukreyev A, & Crowe JE J.r. (2018). Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein. Immunity, 49(2), 363-374.e10.
+ Open protocol
+ Expand
3

Zika Virus Antibody Complement Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant soluble ZIKV E protein was biotinylated and coupled to red fluorescent Neutravidin beads (Life Technologies). Antibodies were diluted to 5 μg/mL in RPMI-1640, and incubated with GP-coated beads for 2 hrs at 37°C. Freshly reconstituted guinea pig complement (Cedarlane Labs) was diluted in veronal buffer with 0.1% fish gelatin (Boston Bioproducts), added to the antibody-bead complexes, and incubated for 20 min at 37°C. Beads were washed twice with PBS containing 15 mM EDTA, and stained with 1:100 dilution of an anti-guinea pig C3 antibody conjugated to Fluorescein isothiocyanate (FITC; MP Biomedicals) for 15 min at ambient temperature. Beads were washed twice more with PBS, and C3 deposition onto beads was analyzed on a BD LSRII flow cytometer and the MFI of the FITC+ of all beads was measured. Z-score values were calculated as described above.
Gilchuk P., Bombardi R.G., Erasmus J.H., Tan Q., Nargi R., Soto C., Abbink P., Suscovich T.J., Durnell L.A., Khandhar A., Archer J., Liang J., Fouch M.E., Davidson E., Doranz B.J., Jones T., Larson E., Ertel S., Granger B., Fuerte-Stone J., Roy V., Broge T., Linnekin T.C., Linde C.H., Gorman M.J., Nkolola J., Alter G., Reed S.G., Barouch D.H., Diamond M.S., Crowe JE J.r., Van Hoeven N., Thackray L, & Carnahan R. (2020). Integrated pipeline for the accelerated discovery of antiviral antibody therapeutics. Nature biomedical engineering, 4(11), 1030-1043.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!