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Purine

Manufactured by Merck Group
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Sourced in Germany

Purine is a laboratory equipment product manufactured by Merck Group. It is used for the purification and isolation of various biological molecules, such as nucleic acids and proteins, through chromatographic techniques. The core function of Purine is to facilitate the separation, identification, and quantification of these biomolecules in research and analytical settings.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (3 mentions) Vinyl chloride, by Merck Group (2 mentions) Ethene, by Merck Group (2 mentions) Peptone, by BD (2 mentions) Yeast extract, by BD (2 mentions) Lambda 35 uv vis spectrometer, by PerkinElmer (2 mentions) 15nh4cl, by Cambridge Isotopes (2 mentions) Dicyanocobinamide cbi, by Merck Group (2 mentions) 5 6 dimethylbenzimidazole dmb, by Merck Group (2 mentions) 5 methylbenzimidazole 5 mebza, by Merck Group (2 mentions) 5 methoxybenzimidazole 5 omebza, by Merck Group (2 mentions) Benzimidazole bza, by Merck Group (2 mentions) Vitamin b12, by Merck Group (2 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Polybrene, by Merck Group (1 mentions) Isopropanol, by Thermo Fisher Scientific (1 mentions) Alanine, by Merck Group (1 mentions) Tyramine, by Merck Group (1 mentions) Pyroglutamic acid, by Merck Group (1 mentions)

4 protocols using purine

1

Lentiviral Knockdown of YPEL3 in gEECs

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Recombinant lentiviral vectors encoding the YPEL3 short hairpin RNA (shYPEL3) and negative control shRNA (shN) (Table S2) were designed by BLOCK-iT™ RNAi designer online software. Virus packaging and cell transfection were performed as described previously [25 (link),26 (link)]. Specifically, recombinant lentiviral vectors were packaged and transduced into HEK 293T cells. After 48 h of continuous culture, the cell supernatant was collected, centrifuged at 2000 r/min for 10 min, then filtered and stored at −80 °C for later use. 3 mL of this virus stock solution collected above was added to 50% density gEECs, and 8μg/μL of polybrene (Sigma, 107689) was added at the same time. After 12 h, the mixture was replaced with normal culture medium, and after 48 h, purine (Sigma, P55805) was added to screen for 4–5 days to select a stable cell line.
Liu J., Qiu R., Liu R., Song P., Lin P., Chen H., Zhou D., Wang A, & Jin Y. (2022). YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats. Animals : an Open Access Journal from MDPI, 12(21), 2973.
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2

Comprehensive Metabolite Identification Protocol

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All reagents employed were of analytical or MS grade. Acetonitrile, methanol, isopropanol, and formic acid were purchased from Thermo Fisher Scientific (Madrid, Spain). Water employed to prepare the running buffer was purified through a Milli-Q system from Millipore (Millipore, Madrid, Spain). Arginine, glutamic acid, pyroglutamic acid, tyrosine, alanine, histidine, cystine, proline, aspartic acid, iminodiacetic acid, 5-hydroxy-L-tryptophan, and 5,6-dihydro-5-methyluracil were acquired in Sigma Aldrich (Madrid, Spain). Methionine sulfone and tyramine used as IS and sodium hydroxide were from Sigma Aldrich while purine and hexakis(2,2,3,3-tetrafluoropropoxy)phosphazine (HP-921) solutions used as a standard mass reference were acquired from Agilent Technologies (Waldbronn, Germany).
Bernardo-Bermejo S., Sánchez-López E., Castro-Puyana M., Benito-Martínez S., Lucio-Cazaña F.J, & Marina M.L. (2020). A Non-Targeted Capillary Electrophoresis-Mass Spectrometry Strategy to Study Metabolic Differences in an In Vitro Model of High-Glucose Induced Changes in Human Proximal Tubular HK-2 Cells. Molecules, 25(3), 512.
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3

Biosynthesis and Purification of Cobamides

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Vitamin B12 (≥98%), dicyanocobinamide (Cbi, ≥93%), 5,6-dimethylbenzimidazole (DMB) (99%), 5-methylbenzimidazole (5-MeBza) (98%), 5-methoxybenzimidazole (5-OMeBza) (97%), benzimidazole (Bza) (98%), purine (98%) were purchased from Sigma-Aldrich. 15NH4Cl (99%) was obtained from Cambridge Isotope Laboratories. Yeast extract and peptone were purchased from Becton, Dickinson and Company. Bza-Cba, 5-MeBza-Cba and 5-OMeBza-Cba were prepared via guided cobamide biosynthesis 10 (link). Factor III (5-OHBza-Cba), norpseudo vitamin B12 and phenolic cobamides (i.e., Phenol-Cba and p-Cresol-Cba) were extracted and purified from Methanosarcina barkeri strain Fusaro (DSM 804), Sulfurospirillum multivorans (DSM 12446) and Sporomusa sp. strain KB-1 (16S rRNA gene GenBank accession no. AY780559.1) cells, respectively. Concentrations of purified cobamides were determined at 361 nm with a Lambda 35 UV-Vis spectrometer (PerkinElmer) using a molar extinction coefficient of 28,060 mole−1 cm−1 12 (link). Ethene (≥99.9%) and vinyl chloride (≥99.5%) were purchased from Sigma-Aldrich. All other chemicals used were reagent grade or higher.
Yan J., Bi M., Bourdon A.K., Farmer A.T., Wang P.H., Molenda O., Quaile A., Jiang N., Yang Y., Yin Y., Şimşir B., Campagna S.R., Edwards E.A, & Löffler F.E. (2017). Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. Nature chemical biology, 14(1), 8-14.
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4

Biosynthesis and Purification of Cobamides

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Vitamin B12 (≥98%), dicyanocobinamide (Cbi, ≥93%), 5,6-dimethylbenzimidazole (DMB) (99%), 5-methylbenzimidazole (5-MeBza) (98%), 5-methoxybenzimidazole (5-OMeBza) (97%), benzimidazole (Bza) (98%), purine (98%) were purchased from Sigma-Aldrich. 15NH4Cl (99%) was obtained from Cambridge Isotope Laboratories. Yeast extract and peptone were purchased from Becton, Dickinson and Company. Bza-Cba, 5-MeBza-Cba and 5-OMeBza-Cba were prepared via guided cobamide biosynthesis 10 (link). Factor III (5-OHBza-Cba), norpseudo vitamin B12 and phenolic cobamides (i.e., Phenol-Cba and p-Cresol-Cba) were extracted and purified from Methanosarcina barkeri strain Fusaro (DSM 804), Sulfurospirillum multivorans (DSM 12446) and Sporomusa sp. strain KB-1 (16S rRNA gene GenBank accession no. AY780559.1) cells, respectively. Concentrations of purified cobamides were determined at 361 nm with a Lambda 35 UV-Vis spectrometer (PerkinElmer) using a molar extinction coefficient of 28,060 mole−1 cm−1 12 (link). Ethene (≥99.9%) and vinyl chloride (≥99.5%) were purchased from Sigma-Aldrich. All other chemicals used were reagent grade or higher.
Yan J., Bi M., Bourdon A.K., Farmer A.T., Wang P.H., Molenda O., Quaile A., Jiang N., Yang Y., Yin Y., Şimşir B., Campagna S.R., Edwards E.A, & Löffler F.E. (2017). Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. Nature chemical biology, 14(1), 8-14.
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