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Fcs express 4 flow cytometry software

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FCS Express 4 Flow Cytometry software is a data analysis and visualization tool for flow cytometry data. It provides users with a comprehensive set of features to analyze and interpret flow cytometry experiments.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (3 mentions) Facsaria 2 high speed cell sorter, by BD (3 mentions) Lgr5 pe, by OriGene (2 mentions) Versene solution, by Thermo Fisher Scientific (2 mentions) Facsaria fusion flow cytometer, by BD (2 mentions) Facsmelody, by BD (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Lsrfortessa flow cytometer, by BD (2 mentions) 40 μm cell strainer, by BD (2 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Colcemid, by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Concanavalin a (cona), by Merck Group (1 mentions) Annexin 5 fluorescein isothiocyanate fitc pi apoptosis detection kit, by Beyotime (1 mentions) Accutase, by Merck Group (1 mentions) 7 aminoactinomycin d, by Thermo Fisher Scientific (1 mentions) Annexin 5 binding buffer, by BD (1 mentions) Annexin 5 pe, by BD (1 mentions) Annexin 5 fitc antibody, by BD (1 mentions) In situ direct dna fragmentation tunel assay kit, by Abcam (1 mentions)

Close spelling variants of this product

FCS Express 4 Flow Cytometry: Research Edition software FCS Express 4 Flow Cytometry FCS Express Flow Cytometry Software FCS Express 4 Cytometry software FCS Express 4 Flow software FCS Express 4 FCS Express software Flow Express software FCS Express FCS software

20 protocols using fcs express 4 flow cytometry software

1

Cell Cycle and Apoptosis Profiling

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For cell cycle assay, the cells were washed with PBS and fixed with cold ethanol for 2 h. Then the cells were stained with propidium iodide (PI) staining solution for 30 min at room temperature in the dark. Then the cells were detected by flow cytometry and the data were analyzed by the FCS 4 Express Flow Cytometry Software (De Novo Software, Glendale, CA, USA). For cell apoptosis assay, the Annexin V-fluorescein isothiocyanate (FITC)/PI Apoptosis Detection kit (Beyotime, shanghai, China) was used. Briefly, the cells were harvested and resupended in binding buffer, and stained with 5 μl of Annexin V-FITC staining solution for 15 min at room temperature in the dark, and then stained with 5 μl of PI staining solution for 5 min at room temperature in the dark. Then the cells were detected by flow cytometry and the data were analyzed by the FCS 4 Express Flow Cytometry Software (De Novo Software, Glendale, CA, USA).
Wang C., Zhu M., Yang D., Hu X., Wen X, & Liu A. (2022). MiR-29a-3p Inhibits Proliferation and Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells via Targeting FOXO3 and Repressing Wnt/β-Catenin Signaling in Steroid-Associated Osteonecrosis. International Journal of Stem Cells, 15(3), 324-333.
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2

Cell Cycle Analysis of ECC-1 and Ishikawa Cells

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The ECC-1 and Ishikawa cells (2.5×105 cells/ well) were cultured with or without NT1044 for 24 hours. The cells were subsequently collected by 0.05% Trypsin (Gibco), washed with PBS, and fixed in a 90% methanol solution. On the day of analysis, the cells were re-suspended in RNA A solution for 30 min at 37 °C, and then stained with PI staining solution for 10 min in the dark. Cell cycle progression was analyzed by Cellometer and analyzed by the FCS 4 Express Flow Cytometry Software (De Novo Software, Glendale, CA, USA). The experiments were repeated three times.
Roque D.R., Zhang L., Wysham W.Z., Han J., Sun W., Yin Y., Livingston J.N., Batchelor K.W., Zhou C, & Bae-Jump V.L. (2021). The Effects of NT-1044, a Novel AMPK Activator, on Endometrial Cancer Cell Proliferation, Apoptosis, Cell Stress and In Vivo Tumor Growth. Frontiers in Oncology, 11, 690435.
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3

Quantifying Immunosuppressive Effects of miPSC-MSCs

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The miPSC-MSC were inactivated by γ-irradiation (30Gy) and plated into a 96-well flat-bottom plate at a concentration of 1 × 105/well 24 hours before the addition of mouse splenocytes prelabelled with 2 μM of carboxyfluorescein diacetate succinimidyl ester ((CFSE) Invitrogen, OR, USA). Splenocytes, from three mouse donors, were cultured in the presence or absence of miPSC-MSC at a 1 : 1 (miPSC-MSC: splenocytes) ratio, in αMEM, P/S, sodium pyruvate, L-glut, 10% FCS, and 1 μg/ml of concanavalin A (Con A; Sigma-Aldrich, MA, USA), an inducer of splenocyte proliferation, for 5 days. Colcemid (Thermo Fisher, MA, USA), a cell cycle arresting agent, was used as a positive control at a concentration of 100 ng/ml. Splenocyte proliferation was analysed by flow cytometry to detect green fluorescence (CFSE), and analysis of cell division and proliferation index (average fold expansion) was achieved using FCS 4 express flow cytometry software (De Novo Software, Los Angeles, CA, USA). Proliferation index in cocultures was expressed as a percentage of PBMC proliferation in the absence of immunomodulatory cells. All experiments were performed in triplicate.
Hynes K., Bright R., Marino V., Ng J., Verma P.J., Gronthos S, & Bartold P.M. (2018). Potential of iPSC-Derived Mesenchymal Stromal Cells for Treating Periodontal Disease. Stem Cells International, 2018, 2601945.
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4

Apoptosis Quantification by Flow Cytometry

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Apoptosis was quantified using an Annexin V-phycoerythrin (PE) conjugate to stain phosphatidylserine externalized during the early phases of apoptosis, and 7-aminoactinomycin D (7-AAD) to indicate the loss of cell membrane integrity in the late phases of apoptosis. Adherent cells were detached using ACCUTASE (EMD Millipore, Temecula, CA) and combined with detached cells that were harvested from the supernatant by centrifugation. Cells were washed with PBS and resuspended in Annexin V Binding Buffer (BD Pharmingen) at a final concentration of 1.0×106 cells/mL. Four μL of Annexin V-PE (BD Pharmingen) was added to 100 μL of cells and incubated in the dark at room temperature for 15 min. Then 7 μL of 7-amino-actinomycin D (7-AAD; eBioscience, San Diego, CA) was added and incubated for 5 min. Cells were diluted with an additional 400 μL of Binding Buffer and analyzed by flow cytometry immediately. Populations of live cells (Annexin V− / 7-AAD-), and those undergoing early- (Annexin V+ / 7-AAD-) and late apoptosis (Annexin V+ / 7-AAD+), were obtained using CellQuest software and quantified using FCS Express 4 Flow Cytometry software (De Novo Software, Los Angeles, CA). Duplicate samples were used for every time point.
Zhu X., Straubinger R.M, & Jusko W.J. (2015). Mechanism-based mathematical modeling of combined gemcitabine and birinapant in pancreatic cancer cells. Journal of pharmacokinetics and pharmacodynamics, 42(5), 477-496.
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5

Apoptosis and Necrosis in Neutrophils

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Apoptosis/necrosis was analyzed in the neutrophil population by flow cytometry (CyFlow Cube 8, PARTEC), as previously described (19 (link)). In brief, neutrophils were stained with an FITC-annexin V antibody (an apoptotic marker, BD Biosciences) and PI (a late apoptotic/necrotic marker, MilliporeSigma), after 2 hours of treatment with Ang II. Data were analyzed by FCS Express 4 Flow Cytometry software (De Novo Software).
Chrysanthopoulou A., Gkaliagkousi E., Lazaridis A., Arelaki S., Pateinakis P., Ntinopoulou M., Mitsios A., Antoniadou C., Argyriou C., Georgiadis G.S., Papadopoulos V., Giatromanolaki A., Ritis K, & Skendros P. (2021). Angiotensin II triggers release of neutrophil extracellular traps, linking thromboinflammation with essential hypertension. JCI Insight, 6(18), e148668.
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6

Flow Cytometry Analysis of Transient and Stable Gene Delivery

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Efficiency of transient and stable gene delivery was estimated by flow cytometry at days 2 and 15 post-nucleofection, respectively. Cell viability was determined at day 2 post-nucleofection. For that, around 1/10 of cell culture volume was collected, washed with PBS, and stained with DAPI/PBS solution. The cells were then subjected to flow cytometry using the BD LSR II Flow Cytometer (BD Biosciences). Gating for Venus signal was accomplished within the DAPI-negative cell population. The results were analyzed using the FCS Express 4 Flow Cytometry software (De Novo Software).
Holstein M., Mesa-Nuñez C., Miskey C., Almarza E., Poletti V., Schmeer M., Grueso E., Ordóñez Flores J.C., Kobelt D., Walther W., Aneja M.K., Geiger J., Bonig H.B., Izsvák Z., Schleef M., Rudolph C., Mavilio F., Bueren J.A., Guenechea G, & Ivics Z. (2018). Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors. Molecular Therapy, 26(4), 1137-1153.
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7

Apoptosis Quantification in Mutant Virus Infection

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The proportion of cells undergoing apoptosis during ANK-R mutant virus infection was determined using TUNEL staining to monitor nuclear DNA degradation and flow cytometric analysis. A549 cells (1×106) were seeded into 6 well plates. The following day, the cells were either mock infected or infected with vMyx-WT, vMyx-MT5KO, vMyx-148–150KO or vMyx-ANKsKO (MOI of 5). Both detached cells in the supernatant and trypsinized adherent cells were collected and pooled together. Fragmented DNA, a marker for apoptotic cell death, was stained using the In situ Direct DNA Fragmentation (TUNEL) Assay Kit (Abcam, ab66108) according to the manufacturer’s protocol. Anti-BrdU APC antibody (eBioscience) was used instead of anti-BrdU Red to allow the simultaneous detection of BrdU and DSred2. The cells were then characterized using a FACScalibur (Becton Dickinson) and analyzed by CELLQuest software. The collected data was analyzed using FCS Express 4 Flow Cytometry software (De Novo Software).
Lamb S.A., Rahman M.M, & McFadden G. (2014). Recombinant myxoma virus lacking all poxvirus ankyrin-repeat proteins stimulates multiple cellular anti-viral pathways and exhibits a severe decrease in virulence. Virology, 0, 134-145.
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8

Apoptosis Assessment of MCF-7 Cells

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Apoptosis was carried out using an annexin V-FITC Kit (Sigma Aldrich, St. Louis, MO).[21 ] Briefly, MCF-7 cells were plated in a sterile 24-well plate at a concentration of 5 × 106 cells/mL. Then, 10 µL of the oil essential sample (final concentration of 40 µg/mL) was added. A solution of hydrogen peroxide (Sigma-Aldrich, St. Louis, MO) (final concentration of 10 µM) was used as the positive control. The plate was incubated at 37°C in 5% CO2 for 72 h. After this period, the cells were colored with fluorescein isothiocyanate-conjugated annexin V and propidium iodide for 15 min at room temperature, protected from light, and then analyzed by flow cytometry. The percentage of positive cells determined over 10,000 acquired events was analyzed by a FACSCalibur system equipped with a 488 nm argon laser and FCS Express 4 Flow Cytometry software (De Novo Software, Los Angeles, CA).[21 ]
de Lima E.M., Cazelli D.S., Pinto F.E., Mazuco R.A., Kalil I.C., Lenz D., Scherer R., de Andrade T.U, & Endringer D.C. (2016). Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity. Pharmacognosy Magazine, 12(Suppl 1), S42-S46.
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9

Immunophenotyping of Cells by Flow Cytometry

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Cells were collected with trypsinization and stained with indicated antibody. For intracellular stains, cells were fixed (BD catalogue # 558049), and permeabilized (BD catalogue # 558050) prior to analysis. Alexa Fluor 647 Mouse Anti-Stat3 (pY705) (Cat# 557815, BD) and Alexa Fluor® 647 Mouse IgG2a, Isotype Control (Cat# 558053, BD) antibodies were used and flow cytometry was performed on BD canto II. The data were analyzed using FlowJO (Tree Star Inc.) software. For surface stains, cells were incubated with human CD133-APC (Cat# FAB11331A-100, R&D systems) conjugated antibody for 30 min at 4 °C and washed once with PBS buffer prior to analysis. Cell sorting was performed using FACSAria II High-Speed Cell Sorter (BD Biosciences, San Jose, CA) and data were analyzed with FCS Express 4 Flow Cytometry software (De-Novo Software, Los Angeles, CA).
Pingali P., Wu Y.J., Boothello R., Sharon C., Li H., Sistla S., Sankaranarayanan N.V., Desai U.R., Le A.T., Doebele R.C., Muldoon L.L., Patel B.B, & Neuwelt A. (2021). High dose acetaminophen inhibits STAT3 and has free radical independent anti-cancer stem cell activity. Neoplasia (New York, N.Y.), 23(3), 348-359.
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10

Quantification of Transfection Efficiency

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Cell supernatants were collected at different time points following transfection. Fresh media was added to the cells after collecting the samples. 50 μl of the supernatants were mixed with 50 μl of the Metridia luciferase substrate (coelenterazine, Invivogen, France). The luciferase activity was determined by measuring emitted light with a luminometer (FLUOstar OPTIMA, BMG LABTECH, Germany). Luciferase activity is expressed in relative light units (RLU).
To determine the number of transfected cells EGFP expression was evaluated by means of fluorescence microscopy (Zeiss Axiovert 200 M, Carl Zeiss Microscopy GmbH, Germany) as well as by flow cytometry (FACSCanto, BD Bioscience, Germany) 24 h post transfection. For flow cytometry analysis, the cells were washed with PBS, detached by exposure to trypsin and suspended in a flow buffer (PBS with 2% FCS, 2mM EDTA, 0,005% NaN3; Sigma Aldrich, Munich, Germany). Dead cells were excluded based on 7-AAD staining (eBioscience, Germany). 10 000 live cells per sample were analysed. Data were analysed with FCS Express 4 Flow Cytometry software (De Novo Software, USA).
Johler S.M., Rejman J., Guan S, & Rosenecker J. (2015). Nebulisation of IVT mRNA Complexes for Intrapulmonary Administration. PLoS ONE, 10(9), e0137504.
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