Anti cd90 clone 5e10

Manufactured by BD

Sourced in United States

The Anti-CD90 (clone 5E10) is a laboratory research reagent that binds to the CD90 (Thy-1) cell surface antigen. It is intended for use in flow cytometry, immunohistochemistry, and other immunological applications to identify and isolate CD90-positive cells. The product is supplied as a solution and the concentration may vary.

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Lab products found in correlation

Anti cd44 clone im7, by BD (1 mentions) Facsaria iiu, by BD (1 mentions) Anti cd45 clone d058 1283, by BD (1 mentions)

Spelling variants of this product

Anti-CD90 (67 citations) CD90 (5E10) (6 citations) CD90 (clone 5E10, (5 citations) FITC mouse anti-human CD90 (clone 5E10; (2 citations)

2 protocols using anti cd90 clone 5e10

Feline MSC Surface Marker Profiling

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To confirm the cell surface markers of feline MSC cultured in bFGF concentrations of 0, 10, 100, and 1000 ng/ml for 5 days, the cells were stained with the following FITC-conjugated: anti-CD14 (clone TUK4, GeneTex Inc., USA), anti-CD29 (clone TS2/16, Thermo Fisher Scientific K.K., Tokyo, Japan), anti-CD44 (clone IM7, BD Biosciences, USA), anti-CD90 (clone 5E10, BD Biosciences), anti-CD105 (clone SN6, Bio-Rad Laboratories, Inc., California, USA) antibodies, and then the cell surface markers were analyzed on a flow cytometer (BD FACSCant II, Becton, Dickinson and Company, USA).

Fujimoto Y., Yokozeki T., Yokoyama A, & Tabata Y. (2020). Basic fibroblast growth factor enhances proliferation and hepatocyte growth factor expression of feline mesenchymal stem cells. Regenerative Therapy, 15, 10-17.

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Flow Cytometry and Cell Sorting of Rhesus Macaque Cells

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Antibodies used for flow-cytometric analysis and fluorescence-activated cell sorting (FACS) of rhesus macaque cells include anti-CD34 (clone 563, BD, Franklin Lakes, NJ), anti-CD45 (clone D058-1283, BD), anti-CD45RA (clone 5H9, BD), and anti-CD90 (clone 5E10, BD). Antibodies were used according to the manufacturer recommendation. Dead cells and debris were excluded via forward scatter/side scatter gating. Flow-cytometric analyses were performed on an Symphony I and FACSAria IIu (BD). Cells for in vitro assays, as well as NHP stem cell transplants, were sorted using a FACSAria IIu cell sorter (BD), and purity was assessed by recovery of sorted cells. CD34⁺CD90⁺CD45RA⁻ sorting for transplantation was performed in yield mode to increase cell recovery, whereas cells for colony-forming cell (CFC) assays were sorted in purity mode to prevent crosscontamination by different subsets.

Radtke S., Colonna L., Perez A.M., Hoffman M., Kean L.S, & Kiem H.P. (2020). Isolation of a Highly Purified HSC-enriched CD34+CD90+CD45RA− Cell Subset for Allogeneic Transplantation in the Nonhuman Primate Large-animal Model. Transplantation Direct, 6(8), e579.

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