Apoptosis was determined with annexin V-FITC/propidium iodide (PI) apoptosis detection kit (Bender MedSystems, Wien, Austria). Following treatment, aliquots containing 5 × 105 cells in 195 µL buffer were stained with 5 µL FITC-conjugated Annexin V for 10 min and after washing and resuspension in 190 µL buffer, cells were stained with 10 µL PI solution. Samples were stored on ice until data acquisition. The analysis was performed by NovoCyte flow cytometer (ACEA Biosciences Inc., San Diego, CA, USA) with Novo Express Software (ACEA Biosciences Inc., San Diego, CA, USA).
Annexin 5 fitc propidium iodide apoptosis detection kit
Manufactured by Thermo Fisher Scientific
Sourced in United States, China, Austria
The Annexin V-FITC/propidium iodide (PI) apoptosis detection kit is a laboratory reagent used to identify and quantify apoptotic cells. It contains Annexin V-FITC, which binds to phosphatidylserine, and propidium iodide, which stains the nucleic acids of cells with compromised membranes. This kit enables the detection and differentiation of early apoptotic, late apoptotic, and necrotic cells.
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Lab products found in correlation
Facscalibur, by BD (2 mentions)
Cytoflex flow cytometer, by Beckman Coulter (2 mentions)
Cell culture supplements, by Thermo Fisher Scientific (2 mentions)
Lsrfortessa flow cytometry, by BD (2 mentions)
Transwell polycarbonate membrane cell culture insert, by Corning (2 mentions)
Caspase 3 activity assay kit, by Thermo Fisher Scientific (2 mentions)
Cell cycle detection kit, by Thermo Fisher Scientific (2 mentions)
Novoexpress software, by Agilent Technologies (1 mentions)
Novocyte flow cytometer, by Agilent Technologies (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Collagenase type 2, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Mitotracker deep red 633, by Thermo Fisher Scientific (1 mentions)
Haucl4, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Accuri c6, by BD (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
Apocynin, by Merck Group (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
31 protocols using annexin 5 fitc propidium iodide apoptosis detection kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Evaluating Cellular Response to Nanoparticles
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2’, 7’-Dichlorofluorescin diacetate (DCFH-DA), Nacetyl cysteine (NAC), Rhodamine 123 (Rho 123), Hoechst 33258 and HAuCl4 were from Sigma (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) was from Gibco (Carlsbad, CA, USA), fetal bovine serum (FBS) was from Sijiqing (Hangzhou, Zhejiang, China). Cell Counting Kit-8 (CCK-8) was from Dojindo (Kumamoto, Japan). Annexin V-FITC/propidium iodide (PI) apoptosis detection kit was from Bender Med-systems (Vienna, Austria). Mitotracker Deep Red 633, Trypsin and type II collagenase were from Invitrogen (Carlsbad, CA, USA). Sodium citrate dehydrate was purchased from Sinopharm Group Chemical Regent Co., Ltd. (Shanghai, China).
3
Adenoviral-Mediated Apoptosis in Prostate Cancer
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Prostate cancer cells C4–2 and LNCaP were cultured in 6-well plates, and tumor cells (1 × 106 cells/well) were treated separately with both CRAd and Adbic adenoviral vectors at different MOI. After 48 h, cells were collected and apoptosis analysis was performed by using the Annexin V-FITC/Propidium iodide (PI) Apoptosis Detection kit (Invitrogen, Carlsbad, CA, USA) according to the procedure provided by the manufacturer. Apoptosis was measured by using FACScalibur™ (Becton Dickinson) flow cytometer.
4
Apoptosis Assay with Annexin V-FITC/PI
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Cell apoptosis was examined using Annexin V-FITC/Propidium Iodide (PI) Apoptosis Detection kit (Invitrogen). In brief, the transfected cells were washed twice with PBS and resuspended with binding buffer. Subsequently, the suspension was reacted with Annexin V-FITC and PI in the dark for 15 min. Next, the apoptosis rate was evaluated using a Flow Cytometer (Beckman Coulter, Miami, FL, USA).
5
Annexin V-FITC/PI Apoptosis Assay
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The Annexin V-FITC/Propidium-Iodide (PI) Apoptosis Detection kit (Invitrogen) was utilized to determine cell apoptosis. Briefly, cells were harvested after transfection for 48 h and washed with phosphate-buffered solution twice. Then, these cells were stained with Annexin V-FITC and PI for 15 min at room temperature, and then immediately analyzed with BD FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ).
6
Quantifying Cell Apoptosis by Annexin V-FITC/PI Assay
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A cell apoptosis assay was performed using an Annexin V-FITC/propidium iodide (PI) apoptosis detection kit (Invitrogen) according to the manufacturer's instructions. Briefly, the cells were collected and washed twice with PBS and then suspended in 300 μl of binding buffer. Annexin V solution (5 μl) was added to the cell suspension and incubated for 15 min in the dark at room temperature. Subsequently, 200 μl of blinding buffer and 5 μl of PI were added and the cell suspension was immediately analyzed on a flow cytometer (Accuri™ C6; BD Biosciences, San Jose, CA, USA). Annexin V−/PI− were viable cells, Annexin V+/PI− cells were early apoptotic cells, and Annexin V+/PI+ cells were late apoptotic or dead cells.
7
Annexin V-FITC/PI Apoptosis Assay
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SK-N-SH cells were placed into six-well plates and resuspended in PBS. Then, AnnexinV-fluorescein isothiocyanate (AnnexinV-FITC)/Propidium Iodide (PI) Apoptosis Detection kit (Invitrogen) was utilized to detect cell apoptosis. Next, the apoptosis rate was monitored using CytoFLEX flow cytometer (Beckman Coulter, Miami, FL, USA). The lower right quadrant (FITC+/PI-) represents apoptotic cells.
8
Annexin V-FITC/PI Apoptosis Assay
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Seventy-two hours after transfection, cell apoptosis was analyzed using the Annexin V-FITC/Propidium Iodide (PI) Apoptosis Detection Kit (Invitrogen) according to the manufacturer's instruction. hucMSCs were stained with Annexin V and PI and detected by using flow cytometry.
9
Annexin V-FITC/PI Apoptosis Assay
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Cell apoptosis was detected using an Annexin V-FITC/propidium iodide (PI) apoptosis detection kit (Invitrogen). The experimental protocol was carried out in strict accordance with the manufacturer’s instructions. Briefly, the cells were cultured in 6-well plates and cultured overnight. After being subjected to the transfection and/or OGD/R treatment as described above, cells were harvested and washed twice with phosphate buffered saline in 1.5 ml tube and 300 μl binding buffer was added to the tube. Then 5 μl Annexin V—FITC was added to the tube and incubated at room temperature in the dark for 30 min. Then, 10 μl PI solution was added to the tube and incubated for 10 min at room temperature in the dark. Cell apoptosis were analyzed using the BD FACSVerse flow cytometer (FACScan; BD Biosciences, Franklin Lakes, NJ, USA).
10
Quantifying Cell Apoptosis by Flow Cytometry
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The treated cells were inoculated into six-well plates and washed with cold PBS. Then, AnnexinVuorescein isothiocyanate (AnnexinV-FITC)/Propidium Iodide (PI) Apoptosis Detection kit (Invitrogen) was used to detect cell apoptosis. Final, Attune NxT Flow Cytometer (Thermo Fisher Scienti c, Waltham, MA, USA) was utilized to monitor the apoptosis rate.
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