Anti cers6 antibodies

Agents were purchased/obtained as follows: (1) bortezomib (Biovision, Palo Alto, CA); (2) fumonisin B1, palmitate, 4-PBA (4-phenylbutyric acid), TUDCA (tauroursodeoxycholic acid), and anti-α-tubulin, anti-HA and anti-CerS2 antibodies (Sigma-Aldrich, St Louis, MO); (3) ceramides (fatty acyl lengths C16, C18, C20, C22, and C24) and C17 ceramide (d_17:1/C_18:0) (Avanti Polar Lipid, Alabaster, AL); (4) anti-GRP78, anti-CHOP, anti-FAS, anti-phospho-eiF2α, anti-eiF2α, anti-PERK, and anti-SCD-1 antibodies (Cell Signaling Technology, Beverly, MA); (5) anti-SREBP-1, anti-phospho-PERK and anti-CerS6 antibodies (Santa Cruz Biotechnology, Santa Cruz, CA); (6) anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) antibody (Millipore, Temecula, CA); (7) anti-INSIG-1 antibody (Abcam, Cambridge, MA); (8) anti-mouse-HRP (horseradish peroxidase) and anti-rabbit-HRP antibodies (Jackson Laboratory, Bar Harbor, ME).

NBD-Sphinganine and fatty acyl CoAs were from Avanti Polar Lipids. Defatted-bovine serum albumin, a protease inhibitor cocktail, anti-HA, anti-CerS2, and anti-tubulin antibodies were from Sigma-Aldrich. Protein A agarose beads and anti-CerS6 antibodies were from Santa Cruz. Horseradish peroxidase was from the Jackson Laboratory. An ECL detection system was from Cyanagen. Silica gel 60 thin layer chromatography plates were from Merck. All solvents were of analytical grade and purchased from Bio-Lab.