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2 protocols using n heptyl β d glucopyranoside

1

Volatile Standards Preparation and Characterization

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The volatile standards used in this study are shown in Additional File 4: Table S1. First, 2,4-dichloroaniline (99.5% purity) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) for the reagents. However, while n-heptyl β-D-glucopyranoside (98% purity) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA), dichloromethane (99.5% purity), liquid chromatography-mass spectrometry-grade methanol, citric acid, sodium phosphates, sodium fluoride (NaF), ascorbic acid, sodium hydroxide (NaOH) and 3-octanol (97% purity) were obtained from Wako Co. Ltd. (Osaka, Japan).
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2

Characterization of Green Coffee Volatiles

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Five green wet-processed coffee samples (C. arabica L.) from different geographic origins (Ethiopia, India, Nicaragua and Rwanda) were supplied by illycaffè S.p.A (Trieste, Italy). Two different commercial lots from Ethiopia were studied and named hereafter Ethiopia 1 and Ethiopia 2. The samples with zero primary and secondary defects, were selected on the basis of standard procedures of sorting and visual aspect, moisture content, screen size, and cup quality. After collection from jute bags, the coffee samples were immediately packed in high barrier plastic bags and stored at room temperature (22–24 °C). n-Heptyl-β-D-glucopyranoside was purchased from Sigma-Aldrich (Milan, Italy); methanol, acetonitrile, formic acid and hexane HPLC analytical grade from Romil LTD (Cambridge, UK). Molecule identity was complemented by the analysis of the pure chemical standards. Linalool, nerol, geraniol, isovaleric acid, 3-methyl-2-butenoic acid, α-terpineol, and β-damascenone were purchased by Sigma Aldrich (Milan, Italy).
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