Cvt 313

Two μmol/L CVT‐313 (Selleck), an effective ATP‐competitive selective CDK2 inhibitor, was incubated for 12 h and used for UMUC3 and T24. Mdivi‐1 (Selleck), a mitochondrial division inhibitor 1, is a selective transmembrane inhibitor of mitochondrial division that inhibits DRP1 and dynamin I (Dnm1). Five μmol/L Mdivi‐1 was incubated with the cell cultures for 24 h. Mito‐Tracker Red (Beyotime) was used at a concentration of 200 μmol/L for 15 min to stain the mitochondria. Nocodazole (Selleck) was applied at a dosage of 100 nmol/L for 12 h to induce the synchronization of cells. Then, the synchronized cells were released in a fresh medium.