Tcrβ chain h57 597

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The TCRβ chain (H57-597) is a primary component of the T cell receptor (TCR) complex. It plays a crucial role in the recognition of antigen-derived peptides presented by major histocompatibility complex (MHC) molecules. The TCRβ chain (H57-597) is a valuable tool for the study of T cell activation and immune responses.

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H57-597 (19 citations) TCRβ (H57-597, (17 citations) APC anti-mouse TCRβ chain (clone H57-597), (2 citations)

2 protocols using tcrβ chain h57 597

Tumor-Infiltrating Immune Cell Analysis

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Twenty-four hours after the last exercise session, mice were anesthetized, and tumors were harvested, and samples were processed as previously described (3 (link)). Flow cytometry data were obtained using an LSRII flow cytometer (BD Biosciences) and/or Aurora (Cytek) and analyzed with FlowJo software. The double/aggregated cells were gated out using forward scatter area (FSC-A) versus forward scatter width (FSC-W) and side scatter area (SSC-A) versus side scatter width (SSC-W). Different fluorophores conjugated with the following mAb were used: CD45 (30-F11, Biolegend), TCRβ chain (H57-597, Biolegend), CD4 (RM4-5, Biolegend), FOXP3 (150D, Biolegend), CD8a (53-6.7, Biolegend), CD11c (N418, Biolegend), Gr1 (RB6-8C5, Biolegend), MHC-II (M5/114.15.2, BD Biosciences) CD11b (M1/70, Biolegend), B220 (RA3-6B2, Biolegend), F4/80 (BM8, Biolegend), IFNγ (XMG1.2, Biolegend), Granzyme B (GB11, Biolegend), Ki67 (16A8, Biolegend), CD62L (W18021D, Biolegend), CD44 (NIM-R8, Biolegend), CD69 (H1.2F3, Biolegend), CXCR3 (173, Biolegend), IL15Rα (6B4C88, Biolegend), IL6Rα (D7715A7, Biolegend), CD247 (Biolegend, 10F.9G2).

Gomes-Santos I.L., Kumar A.S., Hausmann F., Meyer M.N., Shiferaw S.Z., Amoozgar Z., Jain R.K, & Fukumura D. (2024). Exercise intensity governs tumor control in mice with breast cancer. Frontiers in Immunology, 15, 1339232.

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Negative Enrichment of CD8+ T Cells

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For negative enrichment of CD8⁺ T cells, the following biotinylated anti-mouse or anti-mouse/human antibodies were purchased from BioLegend: CD4 (clone RM4–5), CD19 (6D5), B220 (RA3–6B2), CD11b (M1/70), CD11c (N418), CD49b (DX5), and Erythroid cells (TER119). For negative selection of APCs, biotinylated anti-CD4 (RM4–5), CD8a (53–6.7), and Erythroid cells (TER119) were purchased from BioLegend. For flow cytometry, anti-CD126 (clone D7715A7), CD19 (6D5), CD25 (PC61), CD4 (RM4–5), CD44 (IM7), CD45.1 (A20), CD45.2 (104), CD62L (MEL-14), CD8 (53–6.7), Qa-2 (695H1-9-9), and TCR β chain (H57–597) were purchased from BioLegend. Anti-Bcl6 (clone K112–91), CD127 (SB/199), CD200 (OX-90), CD44 (IM7), CD5 (53-7.3), CD62L (MEL-14), CD71 (C2), CD8α (53–6.7), and Helios (22F6) were purchased from BD Biosciences. Anti-CD69 (clone H1.2F3) and FR4 (eBio12A5) were purchased from ThermoFisher Scientific. Streptavidin conjugated to APC (catalog #SA1005) and eFluor 450 (catalog #48-431782) were purchased from ThermoFisher Scientific. For viability, LIVE/DEAD fixable Near-IR, Violet or Yellow (ThermoFisher Scientific) was used according to the manufacturer’s instructions.

Eggert J., Zinzow-Kramer W.M., Hu Y., Tsai Y.L., Weiss A., Salaita K., Scharer C.D, & Au-Yeung B.B. (2023). Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsiveness. bioRxiv.

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