Cell apoptosis was performed with Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining kit (Beyotime Biotechnology, Jiangsu, China). According to the manufacturer’s instruction, the cells were seeded in 6-well plates and incubated for 24 h, followed by transfection for 48 h. Cells were collected, centrifuged, washed twice with PBS, resuspended in 400 µl binding buffer and then incubated with 5 µl Annexin V-FITC and 10 µl PI at room temper-ature for 15 min in the dark. Cells were analyzed for fluorescence with flow cytometer (BD Corp).
Annexin 5 fluorescein isothiocyanate fitc propidium iodide staining kit
Manufactured by Beyotime
Sourced in China
The Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining kit is a laboratory reagent used for the detection and quantification of apoptosis and cell death in cell populations. The kit contains Annexin V-FITC, which binds to phosphatidylserine residues exposed on the surface of apoptotic cells, and propidium iodide, which stains the nuclei of necrotic cells. This combination allows for the discrimination between viable, early apoptotic, and late apoptotic/necrotic cells using flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Resiquimod r848, by Adooq (2 mentions)
Mouse tumor necrosis factor α tnf α, by ABclonal (2 mentions)
Mouse interferon γ ifn γ enzyme linked immunosorbent assay elisa kits, by ABclonal (2 mentions)
Nuclear dye 4 6 diamidino 2 phenylindole dapi the cell counting kit cck 8, by Beyotime (2 mentions)
Membrane dyes of 3 3 dioctadecyloxacarbocyanine perchlorate dio, by Beyotime (2 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Beyotime (2 mentions)
Pluronic f 127, by Merck Group (2 mentions)
4 protocols using annexin 5 fluorescein isothiocyanate fitc propidium iodide staining kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Nanoparticle-Mediated Immunomodulation for Cancer
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Dopamine hydrochloride (98%) was purchased from Aladdin (Shanghai, China). Metformin and chlorine 6 (Ce6) were purchased from HEOWNS Biochemical Technology (Tianjin, China). Pluronic F127 was purchased from Sigma–Aldrich (St. Louis, MO, USA). Resiquimod (R848) was purchased from AdooQ Bioscience (Irvine, CA, USA). DSPE-PEG-MAL was purchased from Ruixi Biotechnology (Xi'an, China). SH-T7 peptide (Sequence: HAIYPRHC) was purchased from Taigu Biotechnology (Nanjing, China). SH-M2 peptide (Sequence: YEQDPWGVKWWYC) was purchased from Source peptide biology (Nanjing, China). Mouse IL-10, mouse IL-6, mouse tumor necrosis factor-α (TNF-α), and mouse interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) kits were provided by ABclonal Biotechnology (Wuhan, China). Nuclear dye 4′,6-diamidino-2-phenylindole (DAPI), the cell counting kit (CCK-8), and annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining kit, membrane dyes of 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1ʹ-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) were purchased from Beyotime Biotechnology (Shanghai, China).
3
Apoptosis Quantification by Flow Cytometry
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Cell apoptosis was performed with an Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining kit (Beyotime Biotechnology, Jiangsu, China). According to the manufacturer’s instruction, the cells were seeded in 6-well plates and incubated for 24 h, followed by the single drug or the combination treatment for an additional 48 h. The cells were collected, centrifuged, washed twice with PBS, resuspended in 400 µL binding buffer, and then incubated with 5µL Annexin V-FITC and 10 µL PI at room temperature for 15 min in the dark. The cells were analyzed for fluorescence with a flow cytometer (BD Corp).
4
Nanoparticle-Mediated Immunomodulation for Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dopamine hydrochloride (98%) was purchased from Aladdin (Shanghai, China). Metformin and chlorine 6 (Ce6) were purchased from HEOWNS Biochemical Technology (Tianjin, China). Pluronic F127 was purchased from Sigma–Aldrich (St. Louis, MO, USA). Resiquimod (R848) was purchased from AdooQ Bioscience (Irvine, CA, USA). DSPE-PEG-MAL was purchased from Ruixi Biotechnology (Xi'an, China). SH-T7 peptide (Sequence: HAIYPRHC) was purchased from Taigu Biotechnology (Nanjing, China). SH-M2 peptide (Sequence: YEQDPWGVKWWYC) was purchased from Source peptide biology (Nanjing, China). Mouse IL-10, mouse IL-6, mouse tumor necrosis factor-α (TNF-α), and mouse interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) kits were provided by ABclonal Biotechnology (Wuhan, China). Nuclear dye 4′,6-diamidino-2-phenylindole (DAPI), the cell counting kit (CCK-8), and annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining kit, membrane dyes of 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1ʹ-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) were purchased from Beyotime Biotechnology (Shanghai, China).
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