Rat anti ctip2 antibody

Manufactured by Abcam

The Rat anti-Ctip2 antibody is a research-use antibody that recognizes the Ctip2 protein in rat samples. Ctip2 is a transcription factor involved in the development and function of the central nervous system. This antibody can be used to detect and analyze Ctip2 expression in rat tissues and cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Mouse anti sqstm1 p62, by Abcam (1 mentions) Rat anti gfap, by Thermo Fisher Scientific (1 mentions) Mouse anti parkin, by Abcam (1 mentions) Rabbit anti lc3b, by Thermo Fisher Scientific (1 mentions) Rabbit anti iba1, by Fujifilm (1 mentions) Chicken anti gfp, by Abcam (1 mentions) Mouse anti brdu, by Merck Group (1 mentions) Vt1000, by Leica (1 mentions) Mouse anti satb2, by Abcam (1 mentions) Rabbit anti aquaporin 4 aqp4 antibody, by Merck Group (1 mentions) Mowiol, by Merck Group (1 mentions) Rabbit anti neun antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Rat anti-CTIP2 Anti-Ctip2 Ctip2

3 protocols using rat anti ctip2 antibody

Immunohistochemical Analysis of Brain Tissue

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fifty micrometers thick floating sections were incubated in blocking solution (PBS, 0.05% bovine serum albumin, 2% fetal bovine serum, 1% Triton X-100 and 0.1% saponin) for 30 min prior to addition of primary antibodies: rat anti-Ctip2 antibody (1:500; Abcam), rat anti-GFAP (1:2000, Invitrogen), chicken anti-GFP (1:1000, Abcam), rabbit anti-Iba1 (1:500, Wako), rabbit anti-LC3B (1:1000, Invitrogen), mouse anti-Parkin (1:200, Abcam), mouse anti-SQSTM1/p62 (1:200, Abcam), rabbit anti-Rab1A (1:200, Proteintech) and mouse anti-Satb2 (1:1000, Millipore) at 4°C overnight. After extensive washes, sections were incubated in appropriate secondary antibodies in blocking solution: Alexa Fluor 488 (1:1000, Invitrogen), and Cy3-conjugated (1:1000, Molecular Probes) for 2 h at room temperature. Antigen retrieval was performed for rat anti-Ctip2 antibody (1:500; Abcam) as previously described (22 (link)). Sections were counterstained with DAPI.

Gautam M., Jara J.H., Sekerkova G., Yasvoina M.V., Martina M, & Özdinler P.H. (2016). Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms. Human Molecular Genetics, 25(6), 1074-1087.

+ Open protocol

+ Expand

Immunohistochemical Staining of Brain Sections

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

E15.5 Embryos were dissected and fixed in 4% PFA overnight. P21 pups were transcardially perfused with PBS, then with 4% PFA, dissected, and postfixed in 4% PFA overnight. Brains were sectioned at 40 μm on a vibrating microtome (Leica VT1000S) and stained as previously described (Lodato et al., 2014 (link)). Primary antibodies and dilutions used were as follows: rat anti-CTIP2 antibody, 1:100 (Abcam ab18465); mouse anti-SATB2, 1:50 (Abcam ab51502); mouse anti-Pvalb, 1:1000 (Millipore MAB); Rabbit anti Olig2 (IBL-18953); Mouse anti Brdu (Millipore MAB); Rabbit anti Ki67(Abcam ab15580) and rabbit anti-CUX1, 1:100 (Santa Cruz CDP M-222). Secondary antibodies and dilutions used were as follows: AlexaFluor 405, 488, 568 and/or 647 secondary antibody (1:750, Life Technologies).

Platt R.J., Zhou Y., Slaymaker I.M., Shetty A.S., Weisbach N.R., Kim J.A., Sharma J., Desai M., Sood S., Kempton H.R., Crabtree G.R., Feng G, & Zhang F. (2017). Chd8 mutation leads to autistic-like behaviors and impaired striatal circuits. Cell reports, 19(2), 335-350.

+ Open protocol

+ Expand

Immunohistochemistry Protocol for Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed as described previously with slight modifications^{38 (link),39 (link)}. Briefly, free-floating sections were permeabilized with 0.3% Triton X-100/PBS, incubated overnight with primary antibodies in 2% bovine serum albumin (BSA)/PBS, and then washed with PBS 3 times for 10 min each. The sections were incubated with secondary antibodies and Hoechst 33342 in 2% BSA/PBS for 2 h, and then washed with PBS 3 times for 10 min each. The sections were mounted on slides with Mowiol (Sigma-Aldrich). Antibodies used were as follows: rabbit anti-aquaporin-4 (AQP4) antibody (Millipore), rabbit anti-glutamine synthetase (GS) antibody (Sigma-Aldrich), mouse anti-GS antibody (Roche), rabbit anti-NeuN antibody (Cell Signaling Technology), mouse CC1 antibody (Calbiochem), rabbit anti-phospho-Smad1/5/8 antibody (Cell Signaling Technology), rabbit anti-Cux1 antibody (Santa Cruz), rat anti-Ctip2 antibody (Abcam), donkey secondary antibodies conjugated with Cy3 (Jackson ImmunoResearch), donkey secondary antibodies conjugated with Alexa Fluor 647 (Molecular Probes) and donkey secondary antibodies conjugated with Alexa Fluor 488 (Molecular Probes).

Morita K., Matsumoto N., Saito K., Hamabe-Horiike T., Mizuguchi K., Shinmyo Y, & Kawasaki H. (2021). BMP signaling alters aquaporin-4 expression in the mouse cerebral cortex. Scientific Reports, 11, 10540.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!