The quinol:cytochrome c oxidoreductase activity of the ACIII was measured spectrophotometrically as described in 29 (link). The reaction was carried out in a 2 ml anaerobic cuvette, at 25 °C in 50 mM potassium phosphate buffer, pH 7.5 in the presence of 50 μM horse heart cytochrome c (Sigma-Aldrich) and 200 μM KCN. Ubiquinol-1 (Q1H2) or reduced vitamin K2 (Sigma-Aldrich) were used as quinol substrates and, in each case, the quinone was reduced using sodium borohydride according to the method by Ragan30 . The reaction was started by the addition of 100 μM of reduced quinone.
Reduced vitamin k2
Manufactured by Merck Group
Reduced vitamin K2 is a laboratory reagent used in various research and analytical applications. It serves as a cofactor in enzymatic reactions and is involved in the regulation of blood coagulation. This product is available in a pure, high-quality form for use in controlled laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using reduced vitamin k2
1
Quinol:Cytochrome c Oxidoreductase Activity Assay
2
Quinol:Cytochrome c Oxidoreductase Activity Assay
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