Anti myhc 2 antibodies clone my 32

Diaphragm and gastrocnemius muscle fibers were identified on 3 μm thick paraffin-embedded sections in all groups of mice and the two time-cohorts. MyHC-I and MyHC-II isoforms were identified using anti-MyHC-I (clone MHC, Biogenesis Inc., Poole, England, UK) and anti-MyHC-II antibodies (clone MY-32, Sigma, Saint Louis, MO), respectively, according to previously published methodologies [4 (link), 6 (link)–8 (link), 18 (link)].

On 3-micrometer muscle paraffin-embedded sections from vastus lateralis muscles of all study groups, MyHC-I and –II isoforms were identified using anti-MyHC-I (clone MHC, Biogenesis Inc., Poole, England, UK) and anti-MyHC-II antibodies (clone MY-32, Sigma, Saint Louis, MO), respectively, as published elsewhere [7] (link), [10] (link), [46] (link). The cross-sectional area, mean least diameter, and proportions of type I and type II fibers were assessed using a light microscope (Olympus, Series BX50F3, Olympus Optical Co., Hamburg, Germany) coupled with an image-digitizing camera (Pixera Studio, version 1.0.4, Pixera Corporation, Los Gatos, CA, USA) and a morphometry program (NIH Image, version 1.60, Scion Corporation, Frederick, MD, USA). At least 100 fibers were measured and counted in each muscle specimen from both study groups.

On 3-micrometer muscle paraffin-embedded sections from gastrocnemius muscle of all study groups, MyHC-I and–II isoforms were identified using anti-MyHC-I (clone MHC, Biogenesis Inc.) and anti-MyHC-II antibodies (clone MY-32, Sigma-Aldrich), respectively, as published elsewhere [8 (link),9 (link),16 (link)].