Allprep dna rna mirna universal isolation kit

Manufactured by Qiagen

The AllPrep DNA/RNA/miRNA Universal isolation kit is a lab equipment product from Qiagen that enables the simultaneous and efficient isolation of genomic DNA, total RNA, and microRNA from a single sample. The kit utilizes Qiagen's proprietary technology to provide a streamlined and reliable solution for comprehensive nucleic acid extraction.

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Allprep dna rna ffpe isolation kit, by Qiagen (1 mentions)

Close spelling variants of this product

AllPrep DNA/RNA/miRNA isolation kit AllPrep DNA/RNA/miRNA Universal AllPrep DNA/RNA isolation kit Allprep RNA isolation kit Allprep RNA/DNA AllPrep Universal Kit AllPrep Isolation Kit MiRNA isolation kit RNA isolation kit DNA isolation kit

2 protocols using allprep dna rna mirna universal isolation kit

Extracting DNA and RNA from FFPE Tissue

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Tumor DNA and RNA was isolated from formalin-fixed, paraffin-embedded (FFPE) sections. A pathologist scored the tumor percentage and indicated the most tumor-dense region on a H&E stain slide for subsequent DNA/RNA isolation. A total of 5–10 FFPE slides (10 µm) were used for simultaneous isolation of DNA and RNA using the AllPrep DNA/RNA FFPE isolation kit (Qiagen, 80234) and the QIAcube, according to the manufacturer’s protocol.
Germline DNA was isolated from peripheral blood mononuclear cells using AllPrep DNA/RNA/miRNA Universal isolation kit (Qiagen, 80224) and the QIAcube, according to the manufacturer’s protocol.

Hoefsmit E.P., Rozeman E.A., Van T.M., Dimitriadis P., Krijgsman O., Conway J.W., Pires da Silva I., van der Wal J.E., Ketelaars S.L., Bresser K., Broeks A., Kerkhoven R.M., Reeves J.W., Warren S., Kvistborg P., Scolyer R.A., Kapiteijn E.W., Peeper D.S., Long G.V., Schumacher T.N, & Blank C.U. (2020). Comprehensive analysis of cutaneous and uveal melanoma liver metastases. Journal for Immunotherapy of Cancer, 8(2), e001501.

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Molecular Profiling of Tumor Samples

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Whole-exome sequencing was performed on pretreatment tumor samples and matched germline DNA from PBMCs. RNA was isolated from tumor samples taken pre-, on- and posttreatment to determine expression of various individual genes as well as immune-related gene signatures.
DNA and RNA were extracted from fresh-frozen samples obtained pre- and posttreatment. For isolation, 10-µm slides were cut in a cryostat. Of the 10-µm slides, one 5-µm slide was cut and stained with H&E to assess tumor percentage. Samples were selected for isolation on the basis of a tumor percentage of at least 10%, except for posttreatment samples from patients with an MPR or pCR. DNA and RNA were isolated simultaneously with the AllPrep DNA/RNA/miRNA Universal Isolation Kit (Qiagen, 80224) by using the QIAcube according to manufacturer’s protocol.

Verschoor Y.L., van de Haar J., van den Berg J.G., van Sandick J.W., Kodach L.L., van Dieren J.M., Balduzzi S., Grootscholten C., IJsselsteijn M.E., Veenhof A.A., Hartemink K.J., Vollebergh M.A., Jurdi A., Sharma S., Spickard E., Owers E.C., Bartels-Rutten A., den Hartog P., de Miranda N.F., van Leerdam M.E., Haanen J.B., Schumacher T.N., Voest E.E, & Chalabi M. (2024). Neoadjuvant atezolizumab plus chemotherapy in gastric and gastroesophageal junction adenocarcinoma: the phase 2 PANDA trial. Nature Medicine, 30(2), 519-530.

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