Maltose

Manufactured by Fujifilm

Sourced in Japan

Maltose is a laboratory equipment product manufactured by Fujifilm. It is a disaccharide composed of two glucose molecules joined by an α-1,4 glycosidic bond. Maltose serves as a primary standard for the determination of enzyme activity and the characterization of carbohydrate-hydrolyzing enzymes.

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Lab products found in correlation

Sucrose, by Fujifilm (2 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions) Xanthan gum, by Fujifilm (1 mentions) Pullulan, by Merck Group (1 mentions) Gibco b 27 supplement, by Thermo Fisher Scientific (1 mentions) Neurobasal medium, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Nacalai Tesque (1 mentions) Triton x 100, by Nacalai Tesque (1 mentions) L glutamine, by Fujifilm (1 mentions) Glycerol, by Fujifilm (1 mentions) 2.5g l trypsin 1mmol l edta solution, by Nacalai Tesque (1 mentions) Penicillin streptomycin mixed solution, by Nacalai Tesque (1 mentions) Raffinose, by Fujifilm (1 mentions) Citric acid, by Merck Group (1 mentions) Fructose, by Fujifilm (1 mentions) Yeast nitrogen base without amino acids, by BD (1 mentions) Zymolyase, by Nacalai Tesque (1 mentions)

Close spelling variants of this product

Maltose monohydrate (7 citations) D(+)-maltose monohydrate (2 citations)

8 protocols using maltose

Feeding Regime for First-Feeding Larvae

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The first-feeding larvae (7 dph) were fed the slurry-type diet, which consisted of 200 mg of G. frondosa extract and 2 g maltose (Wako, Tokyo, Japan) diluted in a measuring cylinder to 10 mL with 0.8% xanthan gum (Wako). For the control, 2 g maltose (Wako) diluted in a measuring cylinder to 10 mL with 0.4% xanthan gum (Wako) was supplied.
About 2,000 larvae at 7 dph, the duration of first feeding, were moved to a 10-L acrylic tank and fed the slurry-type diet on the bottom of the tank as described by Tanaka et al. [1] . After 15 min the feed was washed out. Subsequently, approximately 100–200 larvae were sampled after 10, 20, 30 min, 1, 2, 4, and 8 h into RNAlater (Ambion, Austin, TX, USA) and stored at −20°C until required.

Kawakami Y., Nomura K, & Tanaka H. (2014). Growth Promoting Effect of Hyaluronan Synthesis Promoting Substances on Japanese Eel Leptocephali. PLoS ONE, 9(6), e98688.

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Analyzing Pullulan Hydrolysis by Recombinant PulB

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The hydrolysis products produced by the recombinant PulB protein were analyzed using thin-layer chromatography (TLC). The recombinant PulB (0.4 μM) was incubated with 0.25% pullulan (Sigma, St. Louis, MO), maltotriose (Nakarai tesque, Kyoto, Japan), maltose (Wako, Osaka, Japan), and glucose (Wako) in 50 mM sodium phosphate buffer (pH 6.2) at 37°C for 24 h. Five microliter aliquots of each reaction mixture were spotted onto a Silica gel 60 TLC plate with concentrating zone (Millipore, Darmstadt, Germany). The plate was developed with a 2-propanol/acetic acid/water (4:1:1) solvent system. The plates were dried and sprayed with orcinol-sulphuric acid reagent. The plate was heated at 120°C for 10 min.

Uda A., Sharma N., Takimoto K., Deyu T., Koyama Y., Park E.S., Fujita O., Hotta A, & Morikawa S. (2016). Pullulanase Is Necessary for the Efficient Intracellular Growth of Francisella tularensis. PLoS ONE, 11(7), e0159740.

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Sericin-Based Cell Culture Protocol

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Dulbecco's modified Eagle's medium with high glucose, l-glutamine, phenol red, and sodium pyruvate (D-MEM), DMSO, glycerol, maltose, pure sericin, and all-trans-retinoic acid were purchased from Fujifilm Wako Chemicals Co. Ltd. (Osaka, Japan). Sericin hydrolysate (Seiren Co. Ltd.) with an average molecular mass of 30 kDa was used. Penicillin-streptomycin mixed solution, 2.5 g/L trypsin-1 mmol/L EDTA solution, paraformaldehyde, and tritonX-100 were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Dulbecco's phosphate-buffered saline without Ca²⁺ and Mg²⁺ supplementation (D-PBS-free), neurobasal medium, Gibco B-27 supplement, trypan blue stain (0.4%), bovine serum albumin (BSA), and 5-CFDA-AM (5-carboxyfluorescein diacetate) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). FBS (HyClone, SH30910.03) was obtained from Funakoshi Co., Ltd. (Tokyo, Japan). All other materials and chemicals not specified above were of the highest available grade.

Yamatoya K., Nagai Y., Teramoto N., Kang W., Miyado K., Nakata K., Yagi T, & Miyamoto Y. (2022). Cryopreservation of undifferentiated and differentiated human neuronal cells. Regenerative Therapy, 19, 58-68.

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Carbohydrate Characterization Protocol

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Isomaltose (6-O-α-d-glucopyranosyl-d-glucopyranose) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Panose (6-O-α-glucopyranosyl-maltose) was obtained from Hayashibara (Okayama, Japan). Glucose, maltose, raffinose, and sucrose were purchased from Wako Pure Chemical Industries (Osaka, Japan).

Tsutsumi S., Mochizuki M., Sakai K., Ieda A., Ohara R., Mitsui S., Ito A., Hirano T., Shimizu M, & Kato M. (2019). Ability of Saccharomyces cerevisiae MC87-46 to assimilate isomaltose and its effects on sake taste. Scientific Reports, 9, 13908.

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Comprehensive Analysis of Ginseng Compounds

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Samples of White ginseng and "nine steaming and nine sun-drying" black ginseng were purchased from Liaoning Zhongshutang Black Ginseng Co. (TieLing, China). The standard white and black ginseng extracts were prepared in the laboratory [17 (link)]. Maltol was acquired from J&K Scientific (Beijing, China). 5-hydroxymethylfurfural (5-HMF), D-malic acid, L-malic acid, acetic acid, malonic acid, and succinic acid were procured from Fluka (Seelze, Germany). Citric acid was procured from Sigma (St. Louis, Missouri, USA). Eighteen amino acids, derivatization reagents (2,4-dinitrofluorobenzene), and solid components A and B were acquired from Elite Company (Dalian, China). γ-aminobutyric acid (GABA), dencichin, and dextrose were procured from Dr. Ehrenstorfer (Germany). Fructose, maltose, and sucrose were procured from Wako (Japan). Glacial acetic acid, N, N-dimethylformamide, and phosphoric acid were procured from Komeo (Tianjin, China). Purified water was procured from Watson's (Hong Kong, China). HPLC-grade methanol and acetonitrile were obtained from Oceanpak and Tedia (Fairfield, OH, USA).

Zhang Y., Huang Y, & Dou D. (2024). Anti-prostate cancer mechanism of black ginseng during the "nine steaming and nine sun-drying" process based on HPLC analysis combined with vector space network pharmacology. Discover. Oncology, 15, 12.

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Preparation of Mulberry Leaf Extracts

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(Kyoto, Japan) and used as the preparation of α-glucosidase. Maltose, 1-DNJ, and Glucose CII test were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan).
Fagomine and GAL-DNJ were purified from mulberry leaves as reported previously. 9, 12) Powdered and roasted M. australis leaves were purchased from Urasoe-shi silver human resources center (Urasoe, Japan).
Preparation of extracts of M. australis leaves. Boiled water (50 mL) was added to powdered or roasted M. australis leaves (500 mg) and stirred for 5 min. The solution was suction-filtrated, and the filtrate was collected and used as the leaf extract.

Qiao Y., Nakayama J., Ikeuchi T., Ito M., Kimura T., Kojima K., Takita T, & Yasukawa K. (2020). Kinetic analysis of inhibition of α-glucosidase by leaf powder from Morus australis and its component iminosugars. Bioscience, biotechnology, and biochemistry, 84(10).

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Isolation of 2-DOG-resistant Haploid MCD4

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MCD4 was cultured on Yeast extract Peptone Dextrose (YPD) agar (1.0% yeast extract, 2.0% polypeptone, 2.0% glucose, and 2.0% agar) at 30 °C for 12 h, and then subcultured on sporulation agar (1.0% potassium acetate, 0.1% yeast extract, 0.05% glucose, and 2.0% agar) at room temperature for 1 week. Cell suspension was prepared in 0.1 M phosphate buffer (pH 6.0) containing Zymolyase (Nacalai Tesque, Kyoto, Japan), and after incubation at 30 °C for 1 h, spores were separated using a micromanipulator (Microdissector, Axio Lab, Carl Zeiss, Oberkochen, Germany). The isolates derived from the spores were regarded as MCD4 haploid. The MCD4 haploid isolates were inoculated on maltose minimal agar (2.0% maltose (Wako Pure Chemical Industries, Osaka, Japan), 0.67% Yeast Nitrogen Base without amino acids (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), and 2.0% agar) containing 0.08% 2-DOG and cultured at 30 °C for 10 days. The isolates that could form colonies were determined to be 2-DOG-resistant.

Orikasa Y., Mikumo D, & Ohwada T. (2018). A 2-Deoxyglucose-Resistant Mutant of Saccharomyces cerevisiae Shows Enhanced Maltose Fermentative Ability by the Activation of MAL Genes. Foods, 7(4), 52.

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Oligosaccharide Pyridylamination and Purification

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Maltose was purchased from Wako Chemicals (Osaka). Maltoheptaose was purchased from Tokyo Chemical Industry (Tokyo). A PALSTATION kit and a cellulose cartridge were purchased from TAKARA Bio (Shiga), and used for the pyridylamination of oligosaccharide and purification.
A MonoSpin NH2 column was purchased from GL Science (Tokyo).

Suzuki Y., Okano A., Kabayama K., Nishina A., Tanigawa M., Nishimura K, & Kushi Y. (2016). Purification of Pyridylaminated Oligosaccharides Using 1,2-Dichloroethane Extraction. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 32(5).

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