The following were utilized in this study: G6PD activity assay kit (Abcam #ab176722), PGD activity assay kit (Abcam #ab273328), NETosis assay kit (Cayman #601010), ROS detection assay kit (Cayman #601290), mouse VEGF kit (Thermo Fisher Scientific, #KHG0111 & #MMV00), mouse TNFα kit (Thermo Fisher Scientific, #BMS223-4 & #MTA00B), Phagocytosis assay kit (Cell Biolabs #CBA-222), Mouse Syndecan-1 ELISA kit (Abcam #ab273165), ICAM-1 ELISA kit (R&D Systems #MIC100), CXCL10 ELISA kit (R&D Systems #DY466-05), IL-8 ELISA kit (MyBioSource #MBS7606860), and CXCR2 ELISA kit (MyBioSource #MBS726530). All experiment assays followed the manufacturers’ instructions as done by us previously95 (link). Treated cells were put in a serum-free medium and stimulated with LPS (1 μg/ml) for 3 hrs before assay ROS, VEGF, and TNF.
Cxcl10 elisa kit
Manufactured by R&D Systems
Sourced in United States
The CXCL10 ELISA Kits are quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of CXCL10 in various biological samples. The kit utilizes a specific monoclonal antibody pre-coated onto a microplate. CXCL10 in the sample is bound to the immobilized antibody and then detected using a biotinylated polyclonal detection antibody and streptavidin-peroxidase conjugate.
Automatically generated - may contain errors
Lab products found in correlation
G6pd activity assay kit, by Abcam (1 mentions)
Netosis assay kit, by Cayman Chemical (1 mentions)
Phagocytosis assay kit, by Cell Biolabs (1 mentions)
Mouse syndecan 1 elisa kit, by Abcam (1 mentions)
Icam 1 elisa kit, by R&D Systems (1 mentions)
Il 8 elisa kit, by MyBioSource (1 mentions)
Il 10 elisa kit, by BD (1 mentions)
Ndufb8, by Thermo Fisher Scientific (1 mentions)
Uqcrc2, by Thermo Fisher Scientific (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Lipopolysaccharide lps, by InvivoGen (1 mentions)
Carbonyl cyanide p trifluoromethoxyphenylhydrazone fccp, by Merck Group (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Il 10, by R&D Systems (1 mentions)
In vivo jetpei, by Polyplus Transfection (1 mentions)
Poly a u, by InvivoGen (1 mentions)
5 protocols using cxcl10 elisa kit
1
Immune Response Assays for Cell Activation
2
Cytokine Measurement Protocols
3
Mitochondrial Dysfunction in Immune Signaling
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A list of all reagents, chemicals or kits used in this study can be found in Table S1 . Lipopolysaccharide (LPS) and high molecular weight Poly(I:C) (PIC) were purchased from InvivoGen. MitoTEMPO (MT), N-acetylcysteine (NAC), antimycin A (AA), rotenone (ROT), potassium cyanide, 2-deoxyglucose (2-DG), oligomycin (OM) and carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) were acquired from Sigma-Aldrich while S3QEL-2 was purchased from Cedarlane. IL-1β, IL-6, IL-10, TNF-α, and CXCL10 ELISA kits were purchased from R&D Systems. The IFN-α/IFN-β 2-Plex Mouse ProcartaPlexTM Luminex Panel kit used was from Invitrogen. Tetramethylrhodamine, methyl ester (TMRM), MitoSox Red and CellROX Orange probes were from ThermoFisher. Antibodies against Complex II (SDHB) was from Abcam while antibodies recognizing SOD2 was purchased from Cell Signalling Technology. Antibodies targeting IRF3, pIRF3 (Ser385), IRF7, pIRF7 (Ser477), GPX4, Iκbα, Complexes I (NDUFB8), III (UQCRC2) and IV (COX4) were purchased from ThermoFisher.
4
Poly-IC and Interferon-Based Cancer Immunotherapy
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PICLC (Hiltonol) is a clinical grade formulation of poly-IC stabilized with poly-L-lysine and carboxymethylcellulose manufactured by Oncovir. High molecular weight (hmw) poly-IC and poly-AU were purchased from InvivoGen. Poly-IC/PEI was prepared by mixing hmw poly-IC with in vivo-JetPEI (Polyplus) at a nucleotide:PEI ratio of 6 in a 5% glucose solution. Antimouse PD-L1 monoclonal antibody (mAb) (clone, 10F.9G2) was from BioXcell. Mouse recombinant Interferon-beta and human recombinant Interferon-α2 were purchased from BioLegend. CXCL10 ELISA kits were purchased from R&D Systems. EliSpot plates were from eBioSciences. Dispase I (neutral protease, grade I) was purchased from Sigma-Aldrich. Fluorescence-conjugated Abs for flow cytometry were from Biolegend or eBioscience.
5
Cytokine Quantification via ELISA
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