The effect of the ultrasound contrast agent Lumason® (Bracco Diagnostics, Inc., Milan, Italy) on the ADV transition efficiency and oxygen scavenging was studied by comparing measurements with and without Lumason® co-administered with droplets. Lumason® was activated according to the manufacturer’s package insert and used within 48 h of activation. A 19 G needle (Hamilton, Reno, NV, USA) connected to a 25 µL gas-tight syringe (Becton Dickinson, Franklin Lakes, NJ, USA) was used to withdraw 24 µL of Lumason®. Lumason® was transferred to a 60 mL syringe (Becton Dickinson, Franklin Lakes, NJ, USA) containing 60 mL of 95% DI water and perfluorocarbon droplets (4.8 × 10−4 ± 0.6 × 10−4 mL/mL final concentration). The Lumason® dose was based on the package insert dose per weight (0.03 mL/kg). Co-diluted Lumason® and droplets were infused through the coolant delivery port of an EkoSonic® catheter with a Harvard Apparatus Elite syringe pump (Harvard Apparatus, Holliston, MA, USA) and exposed to ultrasound, as described in Section 2.3 .
60 ml syringe
Manufactured by BD
Sourced in Ireland
The 60 ml syringe is a medical device used for the administration, measurement, and transfer of fluids. It features a cylindrical barrel and a plunger that allows the user to draw in or expel liquid. This syringe has a capacity of 60 milliliters.
Automatically generated - may contain errors
Lab products found in correlation
Lumason, by BD (1 mentions)
Lumason, by Bracco (1 mentions)
Model 7280, by Ugo Basile (1 mentions)
Model 7360, by Ugo Basile (1 mentions)
Fusion 200, by Chemyx (1 mentions)
Med pc version 4, by Med Associates (1 mentions)
Plexiglas operant chambers, by Med Associates (1 mentions)
Light pipe lickometer, by Med Associates (1 mentions)
7 protocols using 60 ml syringe
1
Ultrasound Contrast and Oxygen Scavenging
2
Optimized Cigarette Smoke Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cigarette smoke (CS) extraction protocol was optimised based on previous extraction procedures investigating the effect of CS on both eukaryotic and bacterial cell physiology27 (link),28 (link),41 (link). A 60-mL syringe (Becton Dickinson) fitted with a polycarbonate stopcock 4-way male luer lock (Cole-Parmer), was filled with 10 mL TSB, and connected to one Marlborough cigarette (9–10 mg Tar; 0.7–0.8 mg nicotine). 50 mL of cigarette smoke was aspirated over 5 s, agitated for 15 s and slowly expelled. This process was repeated 8 times (to approximately 1 cm remaining of cigarette). The absorbance (600 nm) of each batch was documented and normalised to 0.35 to ensure consistency between experiments. This 10 mL CS TSB medium (CS-TSB) was defined as 100% CS-TSB. The CS-TSB broth was filtered through a 0.22-µm membrane (Millipore, PVDF low-protein binding membrane) and used immediately. Nicotine and acrolein (Sigma) were added to S aureus cultures grown in TSB at various concentrations to assess their impact on SCV emergence.
3
Stress-Induced Hyperthermia in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experimental mice were single housed during 24 h prior to test, with ad libitum access to food and water. The procedure was adapted from that described previously [14] . Rectal temperature was measured in each mouse twice (T1 = 0 min; T2 = +15 min), both before and after an acute restraint session in a restraint tube (60 ml syringe, Becton Dickinson, Dublin, Ireland). Stress-induced hyperthermia is measured as the difference in temperature between T1 and T2.
4
Thermal Nociception Measurement Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thermal nociception measurements were conducted using the hot plate test (model 7280, Ugo Basile, Comerio, Italy) and the tail flick assay (model 7360, Ugo Basile, Comerio, Italy) as described previously [45] . In the hot plate test, the temperature was 55° C with a cut-off time of 30 s; the mouse was placed on the hot plate and latency to lick a hindpaw or jump was measured. In the tail flick assay, all mice were habituated to a restraint tube (60 ml syringe, Becton Dickinson, Dublin, Ireland) for 20 min, 24 h prior to testing. On the day of testing, mice were placed in the same restraint for 10 min, after which the mouse, in the tube, was placed on the tail flick unit such that a point on the tail approximately 15 mm from the tip was located above the light source. This source, set to an intensity of 90 (range 10-99), was activated by the investigator and latency to flick the tail away from the light beam measured, with a cut-off time of 8 s. For both tests, latency measures were assumed to reflect thermal pain sensitivity.
For each mouse, the hot plate test was performed 30 min prior to the tail flick assay. All assessments were carried out by an investigator who was blind to genotype.
For each mouse, the hot plate test was performed 30 min prior to the tail flick assay. All assessments were carried out by an investigator who was blind to genotype.
5
Microfluidic Device for Tissue-Drug Interaction
6
Operant Chamber Fluid Delivery System
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plexiglas operant chambers (Med Associates) contained a glass drinking spout that was centered on one side of the operant chamber wall at a height of 5 cm from the chamber floor. Tygon tubing connected to the back of the drinking spout would administer the fluid from a 60 ml syringe (BD) hooked up to the syringe in the syringe pump holder. A “light-pipe” lickometer (Med Associates) detected licks via an LED photobeam, and a lick would trigger the syringe pump to deliver one of the three possible fluid volumes via a falling edge transition input to the pump controller. Behavioral protocols were run though Med-PC version IV (Med Associates), and behavioral data were sent via Med Associates superport card TTL (transistor-transistor logic) pulses from the Med-PC software to the syringe pump and subsequently to the Plexon recording system via a solid-state relay.
7
Overfilling Tissue Expanders: Dimensional Changes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Overfill trials were performed using both Natrelle 133 MV (Actavis + Allergan, Parsippany, N.J.) and Mentor Medium Height Style 8200 (Mentor Worldwide, Santa Barbara, Calif.) tissue expanders of indicated capacities ranging from 250 to 800 mL (Refs. 133 MV 11–16 and 354-82 11–16, respectively). Each device was injected by accessing its port with a 21-gauge winged Luer lock needle set (BD, Franklin Lakes, N.J., catalogue no. 367281) and a 60-mL syringe (BD, catalogue no. 309653). Each expander was initially filled to its indicated capacity with normal water and then overfilled in 50-mL increments to 5 times its indicated capacity (ie, 400% overfill). Measurements of each expander’s base diameter (width), height, and projection were made at indicated capacity and with each successive incremental overfill injection. Dimension measurements were obtained using 3-inch standardized calipers (H&H Industrial Products, Chino CA, N.J., UNSPC Code 23241601) with each expander on a flat, level surface. The results of these changes in dimensions were then recorded, collated, and analyzed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!