Rabbit sera for pre-immune and anti-Vpu, mouse anti-p24 monoclonal and anti-CD4 (OKT4) Abs were described previously [18 (link)]. Rabbit serum for anti-BST2 and mouse anti-myc (clone 9E10) Abs used for co-immunoprecipitation were also described previously [14 (link)]. Polyclonal sheep anti-TGN46 and mouse anti-BST2 used in confocal microscopy immunostainings were obtained from Serotec and Abnova, respectively. Rabbit Abs directed against CD4 (Santa Cruz Biotechnology), Myc (Sigma) and actin (Sigma) used for Western blot analyses were obtained from commercial sources as indicated. All secondary Alexa-conjugated immunoglobulin G (IgG) Abs used for flow cytometry and confocal microscopy were obtained from Life Technologies. Paraformaldehyde (PFA) was obtained from Sigma.
Polyclonal sheep anti tgn46
Manufactured by Bio-Rad
Polyclonal sheep anti-TGN46 is a lab equipment product that can be used for the detection of the TGN46 protein. It is a polyclonal antibody raised in sheep against the TGN46 protein.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde pfa, by Merck Group (1 mentions)
Mouse anti flag m2, by Merck Group (1 mentions)
Alexa fluor 568 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Secondary donkey anti rabbit igg alexa fluor488, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg alexa fluor568, by Thermo Fisher Scientific (1 mentions)
Mouse anti ha 11, by Fortrea (1 mentions)
2 protocols using polyclonal sheep anti tgn46
1
Antibody Reagents for Investigating HIV-1 Vpu and BST2 Interactions
2
Immunofluorescence Staining of Cellular Organelles
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The following antibodies were used in the study: mouse anti-Flag M2, mouse anti-EEA1 and polyclonal anti-IL2α (Sigma), mouse anti-HA.11 (Covance), polyclonal sheep anti-TGN46 (AbD Serotec), and polyclonal rabbit anti-CI-M6PR and mouse anti-LAMP1 (Abcam). Secondary donkey anti-rabbit IgG Alexa Fluor488, goat anti-mouse IgG Alexa Fluor568, goat anti-rabbit IgG Alexa Fluor568 and goat anti-sheep IgG Alexa Flour 488 were purchased from Life Technologies.
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