Polyclonal PIPKIγ anti-serum was generated by immunizing rabbits using purified His-tagged mouse PIPKIγ and purified using a human PIPKIγ-conjugated affinity column to generate the anti-pan-PIPKIγ antibody as described 9 (link). To generate the phosphorylated-PIPKIγ antibody, we immunized rabbits with a PIPKIγ phosphopeptide (CDIpYFPTDERSWVYSPLHYSA). The anti-sera were collected, pre-cleaned by a non-phosphopeptide (CDIYFPTDERSWVYSPLHYSA) affinity column, then purified using an affinity column conjugated with the phosphopeptide. Antibodies: anti-HA and MMP9 antibodies (Millipore, Billerica, MA); pERK1/2 and ERK1/2 antibodies (Cell Signaling, Danvers, MA); β-actin antibody and monoclonal anti-α smooth muscle actin antibody (Sigma, St. Louis, MO); Rabbit anti-Cytokeratin 8 antibody, Rat monoclonal anti-CD34 antibody and monoclonal anti-CD68 antibody (Abcam, Cambridge, MA); Alexa Fluor 488 goat anti-mouse antibody, Alexa Fluor 555 goat anti-rabbit antibody and Alexa Fluor 555 goat anti-rat antibody (Molecular probes).
Perk1 2 and erk1 2 antibodies
Manufactured by Cell Signaling Technology
The PERK1/2 and ERK1/2 antibodies are laboratory reagents designed for the detection and analysis of their respective target proteins in biological samples. These antibodies are specific for the phosphorylated forms of PERK1/2 and ERK1/2, which play key roles in cellular signaling pathways. The core function of these antibodies is to enable researchers to study the activation and regulation of these important signaling proteins.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 555 goat anti rabbit antibody, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488 goat anti mouse antibody, by Thermo Fisher Scientific (2 mentions)
Anti ha and mmp9 antibodies, by Merck Group (2 mentions)
β actin antibody and monoclonal anti α smooth muscle actin antibody, by Merck Group (2 mentions)
Rabbit anti cytokeratin 8 antibody, by Abcam (2 mentions)
Rat monoclonal anti cd34 antibody and monoclonal anti cd68 antibody, by Abcam (2 mentions)
Alexa fluor 555 goat anti rat antibody, by Thermo Fisher Scientific (2 mentions)
Akt and p akt, by Cell Signaling Technology (1 mentions)
Anti igf 1r, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Pertussis toxin, by Merck Group (1 mentions)
Pd98059, by Merck Group (1 mentions)
Ki16425, by Merck Group (1 mentions)
Sb203580, by Selleck Chemicals (1 mentions)
Mk 2206, by Selleck Chemicals (1 mentions)
4 protocols using perk1 2 and erk1 2 antibodies
1
Generation of Anti-PIPKIγ Antibodies
2
Western Blotting Protocol for Protein Analysis
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Western blotting was performed as described previously.13 (link) The anti-IGF-1R, anti-IR, and Akt and p-Akt antibodies (Cell Signaling) were used at 1:1,000 dilutions. The p-Erk1/2 and Erk1/2 antibodies (both from Cell Signaling) were used at 1:2,000 dilutions. The anti-GLP-1R (Sigma, St Louis, MO, USA) was used at 8 μg/mL. The control anti-β-actin antibody was diluted at 1:1,000 (Zhongshan Golden Bridge, Beijing, China).
3
Generation of Anti-PIPKIγ Antibodies
4
Signaling Pathways in LPA-Mediated Cellular Responses
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1-Oleoyl-sn-glycero-3-phosphate (LPA), fatty acid-free BSA, pertussis toxin (PTX), PDGF, Ki-16425 and PD98059 were obtained from Sigma-Aldrich Co. LLC. MK-2206 and SB203580 were purchased from Selleck Chemicals. The p-ERK1/2 and ERK1/2 antibodies were purchased from Cell Signaling. YM-254890 was generously provide by Prof. Fumikazu Okajima (Gunma University, Japan). The sources of all other reagents were described in supporting information for detailed descriptions.
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