We used a XYZ3000 distribution platform and CM2000 guillotine cutter to prepare test strips (BioDot, Irvine, CA, USA). A Hitachi F−4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to record the fluorescence spectra. An FIC−S2011−B14 Dry Fluorescence Immunoassay Analyzer was used to read the test strips (Suzhou Hellman Precision Instruments, Suzhou, China). The immunochromatographic strip results were compared with the data obtained using an Agilent 1100 high-performance liquid chromatography system (Agilent Tech, Santa Clara, CA, USA). Hair was crushed with a ball mill (Beijing Wanfu Intelligent Technology Co., Ltd., Beijing, China).
Agilent 1100 high performance liquid chromatography system
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 1100 high-performance liquid chromatography system is a laboratory instrument designed for the separation and analysis of chemical compounds. It is capable of performing high-pressure liquid chromatography (HPLC) to separate and quantify complex mixtures with high precision and accuracy.
Automatically generated - may contain errors
Lab products found in correlation
Cm2000 guillotine cutter, by BioDot (3 mentions)
F 4500 fluorescence spectrometer system, by Hitachi (3 mentions)
Xyz3000 dispensing platform, by BioDot (2 mentions)
C18 ziptip, by Merck Group (1 mentions)
Microtof qiii mass spectrometer, by Bruker (1 mentions)
Mascot search engine, by Matrix Science (1 mentions)
4 protocols using agilent 1100 high performance liquid chromatography system
1
Fluorescence Immunoassay Strip Analysis
2
Fluorometric Test Strip Assay Protocol
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The role of the Hitachi F-4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to read and record the fluorescence pattern. An XYZ3000 dispensing platform and a CM2000 guillotine cutter (BioDot, Irvine, CA, United States) was used to prepare and cut test strips. The purpose of the FIC-S2011-B14 fluorescent strip reader (Suzhou, Jiangsu, China) was used to read and record the results of the fluorometric test strip assay. Agilent 1,100 high performance liquid chromatography system (Agilent Tech, Santa Clara, CA, United States) was used to monitor the results of validated fluorescent test strips.
3
Fluorescent Immunochromatographic Strip Analysis
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An XYZ3000 dispensing platform and CM2000 guillotine cutter (BioDot, Irvine, CA, USA) were used to prepare test strips. The Hitachi F-4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to record the fluorescent spectrum. The FIC-S2011-B14 fluorescent strip reader (Suzhou Helmen Precise Instruments, Suzhou, Jiangsu, China) was used to scan strip results. The immunochromatographic strip results were compared with an Agilent 1100 high-performance liquid chromatography system (Agilent Tech, Santa Clara, CA, USA).
4
iTRAQ Labeling and Peptide Purification
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Peptides were desalted using Zip tip C18 (Millipore, ZTC18S096, USA), concentrated by vacuum centrifuge, and stored at −20°C until further use. iTRAQ labeling was quenched by the addition of 1 M ammonium bicarbonate. Reversed-phase separation was performed on a Dionex U3000 HPLC (Dionex, Sunnyvale, CA). Durashell-C18 reverse phase column (Agela, DC952505-0) was used to purify and concentrate the labeled peptides according to the manufacturer's protocol. LC-MS/MS experiments were performed using a Bruker micrOTOF-Q III mass spectrometer (Bruker Daltonik GmbH, Leipzig, Germany) equipped with a nanospray source and Agilent 1100 high-performance liquid chromatography system (Agilent Technologies, Livermore, CA). The samples were acquired in positive and high-sensitivity mode using electrospray ionization (ESI) method. Peptide sequences were identified from MS/MS fragmentation spectra using the Mascot search engine (Matrix Science, UK). The matched peptides that were considered as potential candidates had the highest Mascot score (≥65) and a peptide sequence coverage of 20 percent of the matched peptide.
The original data were then analyzed using Perseus software (version 1.3.0.4). Proteins whose levels differed by ≥1.2 or ≤0.8 times compared with the controls were used to define differential protein expression.
The original data were then analyzed using Perseus software (version 1.3.0.4). Proteins whose levels differed by ≥1.2 or ≤0.8 times compared with the controls were used to define differential protein expression.
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