125i iodopindolol

Manufactured by PerkinElmer

Sourced in United States

[125I]iodopindolol is a radioligand used in research applications. It is a radiolabeled compound that binds to beta-adrenergic receptors.

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Lab products found in correlation

Parathion, by Chem Service (1 mentions) Diazinon, by Chem Service (1 mentions) Isoproterenol, by Merck Group (1 mentions) Pindolol, by Merck Group (1 mentions) Molecular biology reagents, by Merck Group (1 mentions) Coelenterazine 400a, by Biotrend (1 mentions) Bacteriostatic water, by Abbott (1 mentions) Chlorpyrifos, by Chem Service (1 mentions) Osmotic minipump, by Durect (1 mentions)

3 protocols using 125i iodopindolol

Radiolabeled Iodopindolol Binding Assay

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Animals were purchased from Charles River Laboratories (Raleigh, NC). PerkinElmer Life Sciences (Boston, MA) was the source for [¹²⁵I]iodopindolol (specific activity, 2200 Ci/mmol). Diazinon and parathion were obtained from Chem Service (West Chester, PA) and Sigma Chemical Co. (St. Louis, MO) was the source for all other reagents.

Slotkin T.A., Skavicus S, & Seidler F.J. (2017). DIAZINON AND PARATHION DIVERGE IN THEIR EFFECTS ON DEVELOPMENT OF NORADRENERGIC SYSTEMS. Brain research bulletin, 130, 268-273.

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Molecular Biology Reagents and Assays

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Molecular biology reagents, as well as the tissue culture media and reagents, were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Gibco Invitrogen Corporation (Breda, the Netherlands), unless otherwise specified. [¹²⁵I]-Iodopindolol was obtained from PerkinElmer (Boston, MA, USA). The ligands pindolol and isoproterenol were purchased from Sigma-Aldrich, and recombinant human insulin S100 was from Novo Nordisk A/S (Bagsværd, Denmark). Coelenterazine 400a was purchased from Biotrend Chemikalien GmbH (Köln, Germany). Anti-hemagglutinin (HA) high-affinity rat monoclonal antibodies were from Roche (Basel, Switzerland). Anti-rat horseradish peroxidase (HRP)-conjugated antibodies and anti-rat TRITC-conjugated antibodies were from Sigma-Aldrich.

Mandić M., Drinovec L., Glisic S., Veljkovic N., Nøhr J, & Vrecl M. (2014). Demonstration of a Direct Interaction between β2-Adrenergic Receptor and Insulin Receptor by BRET and Bioinformatics. PLoS ONE, 9(11), e112664.

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Chlorpyrifos-Induced Receptor Changes

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Animals were purchased from Charles River Laboratories (Raleigh, NC) and osmotic minipumps from Durect Corp. (Cupertino, CA). Bacteriostatic water was obtained from Abbott Laboratories (N. Chicago, IL) and PerkinElmer Life Sciences (Boston, MA) was the source for [¹²⁵I]Iodopindolol (specific activity, 2200 Ci/mmol). Chlorpyrifos was obtained from Chem Service (West Chester, PA) and Sigma Chemical Co. (St. Louis, MO) was the source for all other reagents.

Slotkin T.A., Skavicus S, & Seidler F.J. (2015). Prenatal Drug Exposures Sensitize Noradrenergic Circuits to Subsequent Disruption by Chlorpyrifos. Toxicology, 338, 8-16.

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