Anti pe coated magnetic beads

Manufactured by Miltenyi Biotec

Sourced in United Kingdom

Anti-PE coated magnetic beads are a type of laboratory equipment used for the separation and isolation of cells or other biomolecules. The beads are coated with anti-PE (anti-phycoerythrin) antibodies, which can bind to cells or molecules labeled with phycoerythrin. The magnetic properties of the beads allow them to be easily separated from the sample using a magnetic field, facilitating the isolation and purification process.

Automatically generated - may contain errors

Lab products found in correlation

Facsaria, by BD (3 mentions) Rneasy kit, by Qiagen (3 mentions) Erythrocyte lysis buffer, by Qiagen (1 mentions) Anti human cd14 microbeads, by Miltenyi Biotec (1 mentions) Anti mouse igg2a b microbeads, by Miltenyi Biotec (1 mentions) Lymphoprep gradient, by STEMCELL (1 mentions)

Close spelling variants of this product

Anti-PE antibody-coated magnetic beads PE magnetic beads Anti-PE beads Magnetic beads

5 protocols using anti pe coated magnetic beads

Identification and Characterization of iNKT Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thymocytes were stained with PE-conjugated CD1d:PBS57 tetramer, then iNKT cells were positively selected using anti-PE coated magnetic beads (Miltenyi Biotec) and separated using (LS) MACS separation column. Isolated cells were then stained with TCR-β, BV421-CD1d:PBS57 tetramer, CD24, CD44 and NK1.1 to distinguish iNKT cell developmental stages. Gated iNKT cells (CD1d:PBS57⁺ TCR-β⁺) were distinguished in stages using Stage 1 (CD24⁻ CD44^lo NK1.1⁻) Stage 2 (CD24⁻ CD44^hi NK1.1⁻) Stage 3 (CD24⁻ CD44^hi NK1.1⁺). Cells were sorted directly into lysis buffer from RNeasy kit (Qiagen). Sorts were performed with FACSAria (Becton Dickinson) by the Mayo Clinic Flow Cytometry Core Facility.

Thapa P., Romero Arocha S., Chung J.Y., Sant’Angelo D.B, & Shapiro V.S. (2017). Histone deacetylase 3 is required for iNKT cell development. Scientific Reports, 7, 5784.

+ Open protocol

+ Expand

Isolation of GD2+ Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For isolation of GD2+ tumour cells from human and murine tumours, tumours were digested using Type II collagenase, labelled with anti-GD2-PE antibody and bound to anti-PE coated magnetic beads (Miltenyi). Cells were purified according to manufacturer’s instructions (Miltenyi Biotec, Bisley,UK). Purity of GD2+ cells was >98% as confirmed by flow cytometry.

Mussai F., Egan S., Hunter S., Webber H., Fisher J., Wheat R., McConville C., Sbirkov Y., Wheeler K., Bendle G., Petrie K., Anderson J., Chesler L, & De Santo C. (2015). Neuroblastoma arginase activity creates an immunosuppressive microenvironment that impairs autologous and engineered immunity. Cancer research, 75(15), 3043-3053.

+ Open protocol

+ Expand

Isolation of GD2+ Cells from Tumours

Check if the same lab product or an alternative is used in the 5 most similar protocols

For isolation of GD2+ tumour cells from human and murine tumours were digested using Type II collagenase, labelled with anti-GD2-PE antibody (BioLegend) and bound to anti-PE coated magnetic beads (Miltenyi Biotec, Bisley, UK). Cells were enriched according to manufacturer’s instructions to be >98% GD2+ cells as confirmed by flow cytometry using a PE conjugated anti-human GD2 antibody. For isolation of primary GD2+ cells from the bone marrow of diagnosed stage IV patients, bone marrow aspirates were collected in RPMI 1640 media containing 10% FCS. Cells were lysed using erythrocyte lysis buffer (Qiagen) and the white cell fraction isolated by centrifugation. The neuroblastoma cells were labelled with purified mouse anti-human GD2 Clone 14.G2a (BD Pharmingen) and bound to anti-mouse IgG2a/b microbeads (Miltenyi Biotec). Cells were enriched according to manufacturer’s instructions (Miltenyi Biotec). For isolation of monocytes, peripheral blood was collected from healthy donors. Monocytes were separated using a Lymphoprep gradient (STEMCELL Technologies) and enriched by positive selection using anti-human CD14 MicroBeads (Miltenyi Biotec).

Fultang L., Gamble L.D., Gneo L., Berry A.M., Egan S.A., De Bie F., Yogev O., Eden G.L., Booth S., Brownhill S., Vardon A., McConville C.M., Cheng P.N., Norris M.D., Etchevers H.C., Murray J., Ziegler D.S., Chesler L., Schmidt R., Burchill S.A., Haber M., De Santo C, & Mussai F. (2018). Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma. Cancer research, 79(3), 611-624.

+ Open protocol

+ Expand

Isolation and Sorting of Murine iNKT Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

All sorting was performed with a FACSAria (Becton Dickinson). To isolate iNKT cells, thymocytes were labeled with PE conjugated CD1d-PBS57 loaded tetramer. Cells were positively selected with anti-PE coated magnetic beads (Miltenyi Biotec) and separated through an (LS) MACS separation column. Postively selected cells were then labeled with other antibodies cojugated with different fluorochromes to distinguish iNKT cells at different stages of development. For the sort, gated iNKT cells (PBS57-CD1d⁺ TCRβ⁺) were distinguished as Stage 1 (CD24⁻CD44^loNK1.1⁻), Stage 2 (CD24⁻CD44^hiNK1.1⁻) and Stage 3 (CD24⁻CD44^hiNK1.1⁺). Cells were sorted directly into lysis buffer from an RNeasy kit (Qiagen).

Thapa P., Chen M.W., McWilliams D.C., Belmonte P., Constans M., Sant’Angelo D.B, & Shapiro V.S. (2016). NKAP regulates iNKT cell proliferation and differentiation into ROR-γt expressing NKT17 cells. Journal of immunology (Baltimore, Md. : 1950), 196(12), 4987-4998.

+ Open protocol

+ Expand

Identification and Isolation of iNKT Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorts were performed using a FACSAria (Becton Dickinson) in the Mayo Clinic Flow Cytometry Core Facility. Thymocytes were first stained with CD1d-PBS57 loaded tetramer (PE conjugated), then incubated with anti-PE coated magnetic beads (Miltenyi Biotec) to positively select iNKT cells. iNKT cells were then separated using (LS) MACS separation column. Isolated cells were then stained with FVD before surface stains with TCR-β, BV421-CD1d/PBS57 tetramer, CD24, CD44 and NK1.1 to distinguish iNKT cell developmental stages. Gated iNKT cells (PBS57-CD1d⁺ TCR-β⁺) were distinguished in stages: Stage 1 (CD24⁻ CD44^lo NK1.1⁻) Stage 2 (CD24⁻ CD44^hi NK1.1⁻) Stage 3 (CD24⁻ CD44^hi NK1.1⁺). iNKT cells were sorted directly into lysis buffer from RNeasy kit (Qiagen).

Thapa P., Manso B., Chung J.Y., Romera Arocha S., Xue H.H., Angelo D.B, & Shapiro V.S. (2017). The differentiation of ROR-γt expressing iNKT17 cells is orchestrated by Runx1. Scientific Reports, 7, 7018.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!