Streptozotocin (STZ) was obtained from Sigma Chemical Co. (MO, USA). Blood glucose kit, serum creatinine kit, blood urea nitrogen kit, cholesterol kit, triglycerides kit, and urinary albumin kit were purchased from Jiancheng Technology Co. (Nanjing, China). The antibodies against nephrin, α-SMA, β-actin, and goat anti-rabbit IgG-HRP were purchased from Abcam, Inc. (Cambridge, UK). Desmin antibody was purchased from Cell Signaling Technology (MA, USA). CD2 associated protein antibody (CD2AP) was purchased from Signalway Antibody LLC (MD, USA). TGF-β1 and p-smad2/3 antibodies were bought from Bioworld Technology, Inc. (MN, USA).
Desmin antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Desmin antibody is a laboratory reagent used to detect the presence of the desmin protein in cellular samples. Desmin is an intermediate filament protein that plays a structural role in muscle cells. The antibody can be used to identify and visualize desmin-expressing cells in various applications.
Automatically generated - may contain errors
3 protocols using desmin antibody
1
Streptozotocin-Induced Diabetic Kidney Study
2
Detecting Cardiomyocyte Apoptosis via TUNEL
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TUNEL staining was performed as previously described (22 (link)). In brief, 7-μm frozen left ventricular sections were obtained using a Leica, cryomicrotome (Model CM3050S, Leica Microsystems, Buffalo Grove, IL, USA). Sections were stained with an in situ terminal dUTP nick end-labeling (TUNEL) staining kit (11684795910, Roche Diagnostics Corporation, USA) to detect apoptotic cells according to the manufacturer's instructions. Cardiomyocytes were further stained with a Desmin antibody (#4042, Cell Signaling Technology, USA). Nuclei were stained with DAPI. Following coverslip embedding, the sections were imaged through an inverted laser-scanning confocal microscope at ×100 magnification (Zeiss 710, Thornwood, NY, USA). The percentage of TUNEL positive nuclei was quantified using an ImageJ software (30 (link)).
3
Detecting Cardiomyocyte Apoptosis via TUNEL
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