Cells were grown in 24-well plates with 0.170mm glass bottom (In Vitro Scientific). Where indicated, the cells were pre-extracted before fixation with pre-extraction buffer (10 mM PIPES, pH 6.8, 100 mM NaCl, 1.5 mM MgCl2, 300 mM sucrose, 0.5% Triton-X 100, 1mM DTT, 5 μg/ml leupeptin, 2 μg/ml aprotinin, 0.1 mM PMSF) for 20 minutes at 4°C, washed by PBS and then immediately fixed with 4% formaldehyde for 15 minutes at room temperature. Cells were stained with primary antibodies: anti-ubiquitylated conjugateed mouse FK2 antibody (1:500; Enzo, cat. n.: BML-PW8810), anti-VCP (1:500; Abcam; cat. n.: ab11433), anti-NPL4 (1:500; Novus Bio, cat. n.: NBP1-82166), HSP70 (1:100; Enzo, cat. n.: ADI-SPA-830), HSF1 (1:500; Cell Signaling, cat. n.: 4356) anti-ubiquitin lys48-specific (1:500; Merck Millipore, clone Apu2), Sumo2/3 (1:500; Abcam, cat. n.: ab3742), TDP-43 (1:300; Proteintech, cat. n.: 10782-2-AP) and appropriate Alexa Fluor 488 and 568 secondary antibodies (Invitrogen, 1:1000). Cytochrome c was stained by Alexa Fluor 555 conjugated mouse anti-cytochrome c antibody according manufacture’s protocol (BD Pharmingen, cat. n.: 558700).
Clone apu2
Manufactured by Abcam
The Clone Apu2 is a laboratory equipment product. It is designed for a specific core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information about the intended use or features of this product is not available.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 and 568 secondary antibodies, by Thermo Fisher Scientific (2 mentions)
Anti vcp, by Abcam (2 mentions)
Anti npl4, by Novus Biologicals (2 mentions)
Anti ubiquitylated conjugateed mouse fk2 antibody, by Enzo Life Sciences (2 mentions)
Alexa fluor 555 conjugated mouse anti cytochrome c antibody, by BD (2 mentions)
2 protocols using clone apu2
1
Immunohistochemistry of Cellular Stress Markers
2
Immunohistochemistry of Cellular Stress Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were grown in 24-well plates with 0.170mm glass bottom (In Vitro Scientific). Where indicated, the cells were pre-extracted before fixation with pre-extraction buffer (10 mM PIPES, pH 6.8, 100 mM NaCl, 1.5 mM MgCl2, 300 mM sucrose, 0.5% Triton-X 100, 1mM DTT, 5 μg/ml leupeptin, 2 μg/ml aprotinin, 0.1 mM PMSF) for 20 minutes at 4°C, washed by PBS and then immediately fixed with 4% formaldehyde for 15 minutes at room temperature. Cells were stained with primary antibodies: anti-ubiquitylated conjugateed mouse FK2 antibody (1:500; Enzo, cat. n.: BML-PW8810), anti-VCP (1:500; Abcam; cat. n.: ab11433), anti-NPL4 (1:500; Novus Bio, cat. n.: NBP1-82166), HSP70 (1:100; Enzo, cat. n.: ADI-SPA-830), HSF1 (1:500; Cell Signaling, cat. n.: 4356) anti-ubiquitin lys48-specific (1:500; Merck Millipore, clone Apu2), Sumo2/3 (1:500; Abcam, cat. n.: ab3742), TDP-43 (1:300; Proteintech, cat. n.: 10782-2-AP) and appropriate Alexa Fluor 488 and 568 secondary antibodies (Invitrogen, 1:1000). Cytochrome c was stained by Alexa Fluor 555 conjugated mouse anti-cytochrome c antibody according manufacture’s protocol (BD Pharmingen, cat. n.: 558700).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!