Conidia (final concentration, 1 × 105/ml) of the wild-type, AGΔ, GAGΔ, and AG-GAGΔ A. oryzae strains were inoculated and grown as above. The culture broths were filtered through Miracloth (Merck Millipore, Darmstadt, Germany). The mycelia were washed with water twice, dehydrated with tert-butanol, lyophilized, and coated with platinum-vanadium. Mycelia were observed under a Hitachi SU8000 scanning electron microscope (Hitachi, Tokyo, Japan) at an accelerating voltage of 3 kV.
Su8000 scanning electron microscope
Manufactured by Hitachi
Sourced in Japan
The SU8000 scanning electron microscope is a high-resolution imaging tool designed for materials analysis. It features a field emission electron gun, providing high-quality images at a range of magnifications. The SU8000 can capture detailed surface information, enabling users to study the microstructure and composition of a variety of samples.
Automatically generated - may contain errors
Lab products found in correlation
D2 phaser diffractometer, by Bruker (2 mentions)
V 550, by Jasco (2 mentions)
Miracloth, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
β actin antibody, by Keygen Biotech (1 mentions)
9 protocols using su8000 scanning electron microscope
1
Scanning Electron Microscopy of A. oryzae Mutants
2
CO2 Sequestration Using Carbonic Anhydrase
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The CO2 sequestration was performed according to the method reported by Jo et al. [12 (link)]. In a disposable cuvette, 50 µL of SspCA solution was mixed with 450 µL of 1 M Tris containing 20 mM CaCl2, pH = 11. Five hundred microliters of CO2-saturated water prepared at 30 °C was added to initiate the sequestration reaction, which was monitored by measuring A600 using a thermostated spectrophotometer (JASCO V-550) at 30 °C. Instead of the 0 °C used for the hydratase activity assay (Section 2.4 ), CO2-saturated water was prepared at 30 °C because such a temperature is closer to the operation temperature of most CO2-capturing facilities [13 (link),14 (link)]. For characterizing the precipitated CaCO3, the precipitate was collected by filtering it through a 0.45 μm membrane filter, and then dried at 70 °C overnight in an oven before SEM and XRD analysis. The SEM image was obtained with a Hitachi SU8000 scanning electron microscope. The XRD patterns were determined with a Bruker D2 PHASER diffractometer.
3
Cell Sample Preparation for SEM
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SEM analysis was performed using a Hitachi SU8000 scanning electron microscope operated at an acceleration voltage of 1 kV. Sample preparation for SEM examination was performed using critical point drying (CPD) of the cultured cells.
4
Multiwalled CNT Cytotoxicity Evaluation
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Multi-walled CNTs were purchased from Shenzhen Nanotech Port Co., Ltd. (People’s Republic of China). Ver was purchased from Tianjing Centralpharm Co., Ltd. (People’s Republic of China). Dox was obtained from Hisun Pharmaceuticals, Zhejiang, People’s Republic of China. MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], acridine orange (AO) and ethidium bromide (EB) were obtained from Sigma-Aldrich Co., St Louis, MO, USA. Phosphate buffered saline (0.1 M, pH 7.2) was prepared by using double distilled water.18 (link) The P-gp antibody, β-actin antibody, and horseradish peroxidase-conjugated immunoglobulin G antibody were obtained from Nanjing KeyGen Biotech. Inc (Nanjing, People’s Republic of China). All other reagents were of analytical grades. The scanning electron microscope images were obtained by a SU8000 scanning electron microscope (Hitachi Ltd., Tokyo, Japan). The optical density at 492 nm was recorded by the multi-well spectrophotometer reader (Thermo Labsystems Oy, Vantaa, Finland).
5
Scanning Electron Microscopy of Surfaces
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The surface morphology was analysed using a Hitachi SU8000 Scanning Electron Microscope (Hitachi High Technologies Europe GmbH, Krefeld, Germany). Images were taken with an accelerating voltage of 0.7 kV for superficial surface imaging and 10 kV for visualization of the underlying surface.
6
Cryo-SEM Analysis of Gel Microstructure
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Gelled samples were heated inside the hermetically sealed glass vials at 90 °C for 30 min in a water bath and cooled in iced water for 5 min. Then, the microstructure of the gels before and after heat treatment were investigated with cryo-SEM, according to Ong, Dagastine, Kentish, and Gras (Ong et al., 2011 (link)) with modifications. A Hitachi SU8000 scanning electron microscope, equipped with a cryo-preparation system and a vacuum transfer device, was used (Hitachi Ltd, Tokyo, Japan). A piece of the gel was mounted on a copper holder and immersed into a freshly prepared nitrogen slush for 15 s. The frozen sample was then immediately transferred into the cryo-preparation chamber using the vacuum transfer device. The sample was fractured using a chilled scalpel blade in the chamber which was maintained at −120 °C under a high vacuum condition. The sample was then etched at −90 °C for 30 min. No coating was used. Finally, the sample was transferred under vacuum onto a nitrogen gas cooled module, maintained at −110 °C and observed at 2.0 kV. Two replicates were prepared and observed for each experimental condition.
7
Enzyme-Assisted CaCO3 Precipitation for CO2 Sequestration
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The CO2 sequestration was based on the process reported by Jo et al. [18 (link)]. Fifty microliters of R5-SazCA solution or R5-SazCA-SP suspension (the concentration was 0.6 mg/mL for both forms of enzymes) were mixed with 450 µL of 1 M Tris containing 20 mM CaCl2, pH = 11, in a cuvette; CaCO3 precipitation was initiated by the addition of 500 µL of CO2-saturated water prepared at 30 °C. The reaction was monitored by measuring A600 using a thermostated spectrophotometer (JASCO V-550) at 30 °C. An uncatalyzed reaction was also performed by replacing the enzyme solution with storage buffer. In order to obtain sufficient amount of CaCO3 for characterization, the reaction was scaled up to a total volume of 40 mL; after 5 min of incubation, the precipitates were filtered through a 0.45 μm membrane filter, and then subjected to XRD and SEM analysis after overnight drying at 70 °C in an oven. The SEM image was obtained with a Hitachi SU8000 scanning electron microscope. The XRD patterns were determined with a Bruker D2 PHASER diffractometer.
8
Scanning Electron Microscopy of BMDCs
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The sorted BMDCs were harvested and washed with ice-cold PBS twice, and then fixed with phosphate-buffered glutaraldehyde at 4 °C overnight after cultivated with or without cytokine cocktail for 24 h. All samples were observed by a Hitachi SU8000 scanning electron microscope (Marunouchi, Japan).
9
Scanning Electron Microscopy of Material Cross-Sections
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The microstructure of cross-sections taken from the sample was examined using a SU-8000 scanning electron microscope (Hitachi, Hitachi-shi, Japan) in the magnification range of 30–500× at an electron acceleration voltage of 5 kV. The sample cross-sections were attached to the microscope table using a conductive tape and sputter-coated with gold at 10 kV for 10 min using a Gatan high vacuum sputter coater (Pleasanton, CA, USA).
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