12 well polystyrene tissue culture dishes

Rat tail type I collagen, Transwell inserts (0.9 cm² cell culture area, 1.6 × 10⁶ pores/cm²), 12-well polystyrene tissue culture dishes, 12-well polycarbonate plates and Transwell inserts (1.12 cm² cell culture area, 1.6 × 10⁸ pores/cm²) were purchased from Corning (Corning, NY). SB202190 was from Selleckchem (Houston, TX). Collagenase (type IV) was purchased from Worthington Biochemicals (Lakewood, NJ). Y-27632 and N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) were purchased from ApexBio Technology (Houston, TX). Human collagen (type I) was obtained from Advanced Biomatrix (Carlsbad, CA). The ALP assay kit was from AbCam (Cambridge, MA). Gastrin was from Anaspec (Freemont, CA). UGT-Glo™ Assay and P450-Glo™ CYP3A4 Assay were obtained from Promega (Madison, WI). N-acetyl cysteine was purchased from MP Biomedicals (Santa Ana, CA). Murine EGF was procured from Peprotech (Rock Hill, NJ). Primocin was purchased from InvivoGen (San Diego, CA). Nicotinamide, L-alanine p-nitroanilide, fluorescein diacetate, loperamide, zafirlukast, cobalt chloride, potassium phosphate buffer, bovine serum albumin, and A83–01 were acquired from Sigma-Aldrich (St. Louis, MO). All other reagents and Bicinchoninic Acid (BCA) Protein Assay Kit were purchased from Thermo Fisher Scientific (Waltham, MA).

Rat tail type I collagen, Transwell™ inserts, 12-well polystyrene tissue culture dishes, and 12-well polycarbonate filters were purchased from Corning (Corning, NY). Human collagen (type I) was obtained from Advanced Biomatrix (Carlsbad, CA). Collagenase (type IV) was from Worthington Biochemicals (Lakewood, NJ). Gastrin was obtained from Anaspec (Freemont, CA). N-acetyl cysteine was from MP Biomedicals (Santa Ana, CA). Murine EGF was obtained from Peprotech (Rock Hill, NJ). Primocin was purchased from InvivoGen (San Diego, CA). SB202190 was obtained from Selleckchem (Houston, TX). Nicotinamide, A83–01, fluorescein-dextran (70 kDa), atenolol, metoprolol, propranolol, prazosin, doxazosin, digoxin, zosuquidar, and digitoxin were acquired from Sigma-Aldrich (St. Louis, MO). Y-27632 and N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) was from ApexBio Technology (Houston, TX). Red alkaline phosphatase substrate kit was obtained from Vector Labs (Burlingame, CA). Rabbit α-Muc2, ZO-1, and integrin β4 were obtained from Santa Cruz Biotechnology (Dallas, TX). Donkey serum and donkey anti-rabbit or mouse IgG conjugated with Alexa Fluor 488 were from Jackson Immunoresearch (West Grove, PA). Ko143 was purchased from Cayman Chemical (Ann Arbor, MI). All other reagents were from Thermo Fisher Scientific (Waltham, MA).