Three human ESCC cell lines (TE‐8, TE‐9 and TE‐15) were obtained from the RIKEN BioResource Center (Tsukuba, Japan). A short tandem repeat analysis of TE series ESCC cell lines was conducted at RIKEN and the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University (Sendai, Japan). We routinely propagated and prepared the conditioned media of TE series ESCC cell lines (TECM) as described elsewhere.13
Te 15
Manufactured by RIKEN BioResource Center
Sourced in Japan
The TE-15 is a laboratory equipment designed for temperature control. It is capable of maintaining a specific temperature within a designated environment, such as an incubator or a reaction chamber. The TE-15 can be used to regulate the temperature in various experimental setups, where precise temperature control is required.
Automatically generated - may contain errors
Lab products found in correlation
Antibiotic antimycotic, by Thermo Fisher Scientific (3 mentions)
Te 11, by RIKEN BioResource Center (3 mentions)
Antibiotic antimycotic, by Fujifilm (2 mentions)
Te 10, by RIKEN BioResource Center (2 mentions)
Venor gem classic mycoplasma detection kit, by Minerva Biolabs (2 mentions)
Rpmi 1640, by Fujifilm (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Dmem low glucose, by Fujifilm (2 mentions)
Rpmi 1640 medium, by Fujifilm (1 mentions)
Atcc pcs 500 012tm, by American Type Culture Collection (1 mentions)
Kyse70, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kyse170, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Met 5a, by American Type Culture Collection (1 mentions)
Het 1a, by American Type Culture Collection (1 mentions)
Human umbilical vein endothelial cells (huvec), by PromoCell (1 mentions)
Singlequots, by Lonza (1 mentions)
Endothelial basal medium, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Fujifilm (1 mentions)
Human mscs, by American Type Culture Collection (1 mentions)
Human ab serum, by Lonza (1 mentions)
6 protocols using te 15
1
ESCC Cell Line Propagation and Conditioned Media
2
Culturing Esophageal and Mesenchymal Cells
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ESCC cell lines, TE-8, TE-9, TE-10, TE-11, and TE-15, obtained from the RIKEN BioResource Center (Tsukuba, Japan), were maintained in RPMI-1640 medium (Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA) and 1% antibiotic-antimycotic (Invitrogen, Carlsbad, CA, USA). Human bone marrow-derived mesenchymal stem cells (MSCs) were purchased from the American Type Culture Collection (ATCC® PCS-500-012TM; Manassas, VA, USA). MSCs were maintained in low-glucose DMEM (Wako) supplemented with 10% FBS and 1% antibiotic-antimycotic.
3
Esophageal Cell Lines for Research
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Human EC cell lines, KYSE170 and KYSE70, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). TE‐15, TE‐11, TE‐8, and TE‐2 were obtained from RIKEN Bioresource Center (Ibaraki, Japan). HUVEC was purchased from Promocell (Heidelberg, Germany). The human normal esophageal squamous epithelial cell line, HET‐1A, and human mesothelial cell line, MeT‐5A, were purchased from the ATCC (Rockville, MD, USA). These non‐tumor cell lines were used as the control. All EC cell lines, MeT‐5A, and HET‐1A, were maintained in RPMI medium (Nakalai Tisque), supplemented with 10%FBS (System Biosciences). HUVEC was cultured in an endothelial basal medium (Lonza) with endothelial growth supplement Single Quots (Lonza). All cell lines were cultured in an humidified 37°C incubator with 5% carbon dioxide.
4
Characterization of ESCC cell lines
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Five ESCC cell lines (TE-8, TE-9, TE-10, TE-11, and TE-15) were obtained from the RIKEN BioResource Center (Tsukuba, Japan).11 (link) The individuality of the TE series ESCC cell lines was confirmed by a short tandem repeat analysis at RIKEN and at the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University (Sendai, Japan). We purchased the THP-1 human acute monocytic leukemia cell line from the American Type Culture Collection (Mannasas, VA).12 (link) We routinely maintained ESCC cell lines and THP-1 cells and prepared the conditioned media of TE series ESCC cell lines (TECMs) as described.10 (link),13 (link) Mycoplasma was negative in the cell lines by Venor®GeM Classic Mycoplasma Detection kit (Minerva biolabs, Berlin, Germany). To induce macrophage (MΦ)-like differentiation, we also treated THP-1 cells with TPA (Cell Signaling, Danvers, MA) (MΦ-like THP-1 cells) and TECMs for 2 days (TAM-like THP-1 cells) as described.10 (link)
5
Establishing ESCC and MSC cell lines
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Three ESCC cell lines (TE-8, TE-9, and TE-15) were obtained from the RIKEN BioResource Center (Tsukuba, Japan) [15] and maintained in RPMI-1640 (Wako, Osaka, Japan) with 10% FBS (Sigma-Aldrich) and 1% antibioticantimycotic (Invitrogen, Carlsbad, CA). We purchased human MSCs from the American Type Culture Collection (ATCC, Manassas, VA) and maintained them in DMEM low-glucose (Wako) with 10% FBS and 1% antibiotic-antimycotic. All cells were confirmed to be mycoplasma-negative by a Venor ® Gem Classic Mycoplasma Detection kit (Minerva Biolabs, Berlin, Germany). The individuality of the TE series ESCC cell lines was confirmed by a short tandem repeat (STR) analysis at RIKEN and at the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University in 2009 and 2010 (Sendai, Japan). human MSCs were confirmed to be negative for CD14, CD31, CD34, CD45, and CD19, but positive for CD29, CD44, CD90, CD105, CD166, and CD73.
6
Establishment and Characterization of ESCC Cell Lines
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Three ESCC cell lines (TE-8, TE-9, and TE-15) were obtained from the RIKEN BioResource Center (Tsukuba, Japan). The individuality of the TE series ESCC cell lines was confirmed by a short tandem repeat analysis at RIKEN and at the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University (Sendai, Japan). We routinely maintained the ESCC cell lines in RPMI-1640 (Wako, Osaka, Japan) with 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% antibiotic-antimycotic (Invitrogen, Carlsbad, CA, USA). TECMs were prepared by plating 5 × 10 6 tumor cells in 10 ml complete medium in 100 mm dishes for 24 h, and thereafter changing the medium to complete DMEM (Wako) supplemented with 10% human AB serum (Lonza, Walkersville, MD, USA) instead of FBS. After 2 days, the supernatants were harvested, centrifuged, and stored in aliquots at -80 °C.
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