Methanol, acetonitrile, isopropanol, formic acid, and ammonium acetate were purchased from Thermo Fisher Scientific (Fair Lawn, NJ). Ammonium bicarbonate (LC-MS grade) and methyl tert-butyl ether (MTBE) were purchased from Sigma-Aldrich (St. Louis, MO). Ultra-pure water (18.2 m ω cm) was prepared in house by a Milli-Q purified water system (Merck KGaA, Darmstadt, Germany).
Milli q purified water system
Manufactured by Merck Group
Sourced in Germany
The Milli-Q purified water system is a laboratory water purification device that produces ultrapure water. It utilizes a combination of filtration and deionization techniques to remove impurities and contaminants, resulting in water that meets the highest purity standards for laboratory and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (6 mentions)
Acetonitrile, by Thermo Fisher Scientific (5 mentions)
Methanol, by Thermo Fisher Scientific (4 mentions)
Ammonium acetate, by Thermo Fisher Scientific (3 mentions)
Methyl tert butyl ether (mtbe), by Merck Group (2 mentions)
Isopropanol, by Thermo Fisher Scientific (2 mentions)
Ammonium bicarbonate lc ms grade, by Merck Group (2 mentions)
Acquity uplc 1 class, by Waters Corporation (1 mentions)
Centrifuge 5417r, by Eppendorf (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Ammonium hydroxide, by Thermo Fisher Scientific (1 mentions)
Lc ms grade methanol, by Thermo Fisher Scientific (1 mentions)
5 protocols using milli q purified water system
1
Metabolite Extraction Reagents Purchase
2
High-resolution mass spectrometry protocol
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A Triple TOF® 6600 high-resolution mass spectrometry system (AB SCIEX, USA) equipped with an ACQUITY UPLC I-Class ultrahigh-performance liquid system (Waters, USA) and Analyst® TF data acquisition software (AB SCIEX, USA) was used. We used a 5417R centrifuge (Eppendorf, Germany); Vortex Mixer T1 vortex oscillator (Titan SCIENTIFIC LAB); TIMI-10K micromini centrifuge (Titan SCIENTIFIC LAB); and a Labconco centrifugal concentrator (Labconco, USA). Ultrapure water (18.2 MΩ•cm) was prepared using a Milli-Q purified water system (Merck Millipore, USA).
3
Bile Acids Quantification Protocol
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Mass spectrometry level methanol, acetonitrile, isopropanol, formic acid and ammonium acetate were purchased from Fisher Scientific (Fair Lawn, United States). Mass spectrometry level ammonium bicarbonate and methyl tert-butyl ether (MTBE) were purchased from Sigma-Aldrich (St. Louis, United States). Ultra-pure water (18.2 m Ω cm) was used to prepare using Milli-Q purified water system (Merck KGaA, Darmstadt, Germany). Reference bile acid standards and isotope internal standards were purchased from Avanti Polar Lipids (Alabama, United States), Cayman Chemical (Ann Arbor, United States), Cambridge Isotope Laboratories Inc. (Tewksbury, United States), IsoSciences (Ambler, United States), Sigma-Aldrich (St. Louis, United States) and Toronto Research Chemicals (Toronto, Canada). For more information about standards, please referred to Supplementary Table S1 .
4
Mass Spectrometry Reagent Preparation
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Mass spectrometry methanol, acetonitrile, and formic acid were purchased from Fisher Scientific, Fair Lawn, NJ, USA. Mass spectrometry ammonium bicarbonate was purchased from SIGMA-ALDRICH (Sigma-Aldrich Co., St. Louis, MO, USA). 2-Octanol (≥99.5%) was purchased from TCI Chemical Industry Development (Shanghai, China). Ultrapure water (18.2 M Ω·cm) was prepared using the Milli-Q purified water system (Merck KGaA, Darmstadt, Germany).
5
Lipidomics Mass Spectrometry Protocol
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LC/MS-grade methanol, acetonitrile, isopropanol, formic acid, ammonium acetate and ammonium hydroxide were purchased from Fisher Scientific (Fair Lawn, USA). LC/MS-grade ammonium bicarbonate and LC-grade methyl-tert-butyl-ether (MTBE) were purchased from Sigma-Aldrich Co. (St. Louis, USA). Isotopic internal standards were purchased from Cambridge Isotope Laboratories Inc. (Tewksbury, USA) and Toronto Research Chemicals (Toronto, Canada). A lipid mixture of SPLASH LIPI-DOMIX isotopic internal standard was purchased from Avanti Polar Lipids (Alabama, USA). Ultrapure water (18.2 MO cm) was prepared with an in-house Milli-Q Purified Water system (Merck KGaA, Darmstadt, Germany).
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