Formalin-fixed, paraffin embedded sections of mouse and human organs were cut into 6 μm thin sections, deparaffinized and thereafter rehydrated. Epitope retrieval was then performed in 10mM Tris-EDTA-buffer pH 9,0 during 4 min in microwave oven 850W followed by 15min 150W. After cooling down for 20 min at RT, samples were rinsed properly in water. Concerning the staining procedure, the slides were firstly blocked for 10 min in 3% BSA in PBS. After rinsing in Tris-HCl pH 7,4, incubation of primary antibody was done for 60 min in 3% BSA/PBS. Following few rinses, appropriate secondary antibody (Dako EnVision anti-rabbit or anti-mouse) incubation was done for 30 min. Slides, again rinsed, were then incubated in DAB+ liquid Dako (K3468) for 10 min, and then rinsed in water. Samples were incubated in Mayers HTX for 1 min, rinsed with tap water, and finally dehydrated, cleared and mounted. Human testicular samples were obtained from patient diagnosed testicular neoplasm and therefore underwent orchiectomy.
Envision anti rabbit or anti mouse
Manufactured by Agilent Technologies
Sourced in Denmark
The EnVision anti-rabbit or anti-mouse is a lab equipment product designed for detection and quantification of target proteins in immunoassays. It provides a reliable and sensitive method for signal amplification, enabling researchers to accurately measure protein levels in their samples.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using envision anti rabbit or anti mouse
1
Immunohistochemistry of Mouse and Human Tissues
2
Assessing Liver Inflammation via F4/80 Immunostaining
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For the evaluation of liver inflammation, F4/80 immunostaining was performed. OCT frozen livers were cut in 8 µm-thick sections, unmasked according to the primary antibody to be used and subjected to peroxide blocking, 3% (v/v) H2O2 in PBS, during 10 min at RT. For stainings, samples were blocked with goat anti-mouse FAB fragment (Jackson Immunoresearch; USA), and blocked with 5% (v/v) goat serum. Then, sections were incubated with the primary antibody in DAKO antibody diluent in a 1:50 dilution during 1 h at 37 °C, followed by Envision anti-rabbit or anti-mouse (DAKO; Denmark) HRP-conjugated secondary antibody incubation. Colorimetric detections were confirmed with vector VIP chromogen (Vector; USA) and sections were counterstained with hematoxylin. Samples were mounted using DPX mounting medium. For the analysis, images were taken with an upright light microscope. Representative micrographs were taken under 20x objective. Stained area percentage of each sample were calculated using FRIDA 1.0 software.
3
Immunohistochemistry of Mouse and Human Tissues
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Formalin-fixed, paraffin embedded sections of mouse and human organs were cut into 6 μm thin sections, deparaffinized and thereafter rehydrated. Epitope retrieval was then performed in 10mM Tris-EDTA-buffer pH 9,0 during 4 min in microwave oven 850W followed by 15min 150W. After cooling down for 20 min at RT, samples were rinsed properly in water. Concerning the staining procedure, the slides were firstly blocked for 10 min in 3% BSA in PBS. After rinsing in Tris-HCl pH 7,4, incubation of primary antibody was done for 60 min in 3% BSA/PBS. Following few rinses, appropriate secondary antibody (Dako EnVision anti-rabbit or anti-mouse) incubation was done for 30 min. Slides, again rinsed, were then incubated in DAB+ liquid Dako (K3468) for 10 min, and then rinsed in water. Samples were incubated in Mayers HTX for 1 min, rinsed with tap water, and finally dehydrated, cleared and mounted. Human testicular samples were obtained from patient diagnosed testicular neoplasm and therefore underwent orchiectomy.
4
Immunohistochemical Analysis of PWP1 in HNSCC
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Formalin-fixed, paraffin-embedded sections of human HNSCC tumor specimens were cut into 6 mm thin sections, deparaffinised and thereafter rehydrated. Epitope retrieval was then proceeded in 10 mM Tris-EDTA-buffer (pH 9) during 4 min in microwave oven 4 min 850 W followed by 15 min at a lower power (150 W). After blocking with 3% BSA PBS for 10 min the slides were rinsed in Tris-HCl (pH 7.4), and incubated overnight with primary antibody against PWP1 (1:100 Life Technologies). Control slides were incubated with normal nonimmunized appropriate animal serum. The samples were then incubated appropriate secondary antibody (Dako EnVision anti-rabbit or anti-mouse) for 30 min and 10 min in DAB+ liquid Dako (K3468).
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