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Fingerprinting 2

Manufactured by Bio-Rad
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Sourced in United States, France

The Fingerprinting II is a laboratory equipment used for DNA profiling. It is designed to perform electrophoresis and analysis of DNA samples.

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Lab products found in correlation

Chef dr 3 system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Fingerprinting II version 3.0 software Fingerprinting II v3.0 software Fingerprinting II v4.5 software Fingerprinting II Informatix Software Fingerprinting II software

2 protocols using fingerprinting 2

1

Genetic Relatedness Profiling by PFGE

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Pulsed-field gel electrophoresis was used to assess genetic relatedness. Plug preparation was performed as described earlier [45 (link)], plugs were digested with ApaI, and macrorestriction profiles were obtained in a CHEF DRIII instrument with electrophoresis parameters following Chevallier et al. [46 (link)]. Profiles were analyzed using the software Fingerprinting II (BioRad, Hercules, CA, USA) using the Dice similarity coefficient and the UPGMA clustering method. The threshold for probable relatedness was set at 85%.
Kardos G., Sárközi R., Laczkó L., Marton S., Makrai L., Bányai K, & Fodor L. (2022). Genetic Diversity of Actinobacillus pleuropneumoniae Serovars in Hungary. Veterinary Sciences, 9(10), 511.
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2

PFGE analysis of bacterial isolates

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PFGE using ApaI as a restriction enzyme was done as described previously [36] (link). DNA fragments were separated in CHEF-DRIII system (Biorad, Marne LA Coquette, France) at 6V/cm and 14°C for 21 hours with pulse times ranging from 3 s to 20 s. Computer-assisted analysis was performed by using fingerprinting II (Biorad, Marne LA Coquette, France) with the unweighted pair-group method with artithmetic averages (UPGMA) and Dice similarity coefficient for banding pattern comparison. A PFGE type was defined by a cluster of isolates showing ≥80% similarity
Rafei R., Dabboussi F., Hamze M., Eveillard M., Lemarié C., Gaultier M.P., Mallat H., Moghnieh R., Husni-Samaha R., Joly-Guillou M.L, & Kempf M. (2014). Molecular Analysis of Acinetobacter baumannii Strains Isolated in Lebanon Using Four Different Typing Methods. PLoS ONE, 9(12), e115969.
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