Log In Sign up
The largest database of trusted experimental protocols

Human il 10 high sensitivity elisa kit

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Human IL-10 High Sensitivity ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the concentration of human interleukin-10 (IL-10) in biological samples. The kit utilizes a highly sensitive and specific antibody pair to detect IL-10 levels with a wide dynamic range.

Automatically generated - may contain errors

Lab products found in correlation

Human tsg 6 elisa kit, by RayBiotech (1 mentions) 3 kda cutoff filter, by Merck Group (1 mentions)

Close spelling variants of this product

Human IL-10 ELISA kit High sensitivity ELISA Human IL-10 IL-10 ELISAs

2 protocols using human il 10 high sensitivity elisa kit

1

Quantifying Anti-Inflammatory Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-10 and TGF-B1 were used as markers of anti-inflammatory activity. IL-10 and TGF-B1 abundance were evaluated in the secretome obtained from 1 × 106 activated and 1 × 106 non-activated MSCs using the human IL-10 High sensitivity ELISA Kit (Invitrogen) and TGF-B1 ELISA Kit (Life Technologies), respectively.
Quintanilla M.E., Ezquer F., Morales P., Santapau D., Berríos-Cárcamo P., Ezquer M., Herrera-Marschitz M, & Israel Y. (2019). Intranasal mesenchymal stem cell secretome administration markedly inhibits alcohol and nicotine self-administration and blocks relapse-intake: mechanism and translational options. Stem Cell Research & Therapy, 10, 205.
+ Open protocol
+ Expand
2

Secretome Analysis of MSC Spheroids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three days after MSC-spheroid formation, the secretome was obtained by harvesting the culture media. The media was centrifuged at 400 × g for 10 min to remove whole cells and at 12,000 × g for 20 min to remove cell debris. As control, secretome of the same number of 2D-cultured MSCs was obtained and concentrated using 3 kDa cutoff filters (Millipore, Midlesex, MA) to reach to the same volume of MSC-spheroid secretome. IL10 and TSG6 abundance were evaluated in the secretomes using the human IL10 High sensitivity ELISA kit (Invitrogen) and the human TSG6 ELISA kit (RayBio, Norcross, GA) respectively.
Ezquer F., Morales P., Quintanilla M.E., Santapau D., Lespay-Rebolledo C., Ezquer M., Herrera-Marschitz M, & Israel Y. (2018). Intravenous administration of anti-inflammatory mesenchymal stem cell spheroids reduces chronic alcohol intake and abolishes binge-drinking. Scientific Reports, 8, 4325.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!