Freestyle freedom

Male Wistar rats (Charles River Laboratories International, Inc, Wilmington, MA) were made diabetic using a single intraperitoneal dose of streptozotocin (58 mg ˟ kg⁻¹) (Sigma-Aldrich, St Louis, MO). streptozotocin solution was made in citrate buffer immediately before administration. Induction of diabetes was confirmed by measuring blood glucose levels in samples obtained from tail pricking and use of a portable glucose meter between 9 AM and noon (FreeStyle Freedom, Abbott Diabetes Care Inc, Alameda, CA) (Dave et al. 2011b (link)). After induction of diabetes, blood glucose levels were monitored twice a week. The data shown in Figure 1B for groups of diabetic animals are last readings of untreated diabetes; i.e., levels of blood glucose just prior to insulin pellet implantation. Animals having blood glucose levels >310 mg × dl⁻¹ after streptozotocin injection were considered diabetic.

Streptozotocin (Sigma-Aldrich, St Louis, MO), the β-cell toxin, was intraperitoneally administered at a dose of 58 mg × kg^-1 to induce diabetes in male Wistar rats (Charles River Laboratories International, Inc, Wilmington, MA). Streptozotocin was dissolved in citrate buffer and prepared immediately before use. After induction of diabetes, rats were monitored twice a week for their blood glucose levels by tail pricking using a portable glucose meter between 9 AM and noon (FreeStyle Freedom, Abbott Diabetes Care Inc, CA) (Dave et al., 2011b (link)). The results presented in Figure 2A for diabetic groups are blood glucose levels at the time of insulin pellet implantation (i.e. at last reading of untreated diabetes). The animals were considered diabetic if their blood glucose levels was >300 mg × dl^-1 after Streptozotocin administration.

Male Wistar rats were made diabetic by intraperitoneal injection (i.p) of the β cell toxin streptozotocin (STZ; (58 mg / kg body weight; Sigma Aldrich, St Louis, MO) (Dave, et al., 2011 (link)). After STZ injection, blood glucose levels were measured (using portable glucose meter: Freestyle Freedom, Abbott Diabetes Care Inc., Alameda, CA, USA; detection range 20–550 mg/dL) in non-fasting rats by tail pricking twice a week. Blood glucose values of >550 mg/dL were assigned a value of 550 mg/dL for statistical analysis.