Cd20 l27

Manufactured by BD

The CD20 (L27) is a laboratory equipment product. It serves as an analytical tool for the detection and quantification of CD20 protein expression on the surface of cells. The core function of the CD20 (L27) is to provide researchers and clinicians with accurate and reliable data on the levels of CD20, a key marker in various immunological and hematological applications.

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Lab products found in correlation

Cd11c b ly6, by BD (4 mentions) Cd14 m5e2, by BD (3 mentions) Cd1c ad5 8e7, by Miltenyi Biotec (3 mentions) Cd19 hib19, by BioLegend (3 mentions) Cd123 7g3, by BD (3 mentions) Cd141 ad5 14h12, by Miltenyi Biotec (3 mentions) Cd66abce tet2, by Miltenyi Biotec (3 mentions) Cd3 sp34 2 and sk7, by BD (2 mentions) Cd3 sk7, by BD (2 mentions) Lsrfortessa flow cytometer, by BD (2 mentions) Cd16 3ge, by BioLegend (2 mentions) Cd56 hcd56, by BD (2 mentions) Lsrii flow cytometer, by BD (1 mentions) Cd80 2d10, by BioLegend (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Cd16 3g8, by BioLegend (1 mentions) Cd45 hi30, by BioLegend (1 mentions) Cd56 hcd56, by BioLegend (1 mentions) Ccr7 g043h7, by BioLegend (1 mentions) Fcr block, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

CD20 APC-Cy7 (L27) (3 citations) Anti-CD20 (clone L27, (2 citations) Anti-CD20-APC-Cy7 (clone L27, (2 citations) CD20 V450-clone L27 (2 citations) CD20 APC (clone L27) (2 citations) Allophycocyanin-H7 anti-CD20 (clone L27, (2 citations)

4 protocols using cd20 l27

Multiparameter Flow Cytometry Panel

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Cell suspensions were incubated with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies). Fc receptors were blocked using FcR block (Miltenyi) followed by staining with antibodies against surface molecules CD1c (AD5‐8E7; Miltenyi), CD3 (SP34‐2 and SK7; BD), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3G8; Biolegend), CD19 (HIB19; Biolegend), CD20 (L27; BD), CD45 (HI30; Biolegend), CD56 (HCD56; Biolegend), CD66abce (TET2; Miltenyi), CD80 (2D10; Biolegend), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi), CCR7 (G043H7; Biolegend), and HLA‐DR (G46‐6; BD) for 15 min at 4°C in PBS with 2% FCS and fixed with 1% paraformaldehyde. Cells were analyzed using an LSRII flow cytometer (BD) and data were analyzed using FlowJo X software (Tree Star).

Lepzien R., Rankin G., Pourazar J., Muala A., Eklund A., Grunewald J., Blomberg A, & Smed‐Sörensen A. (2019). Mapping mononuclear phagocytes in blood, lungs, and lymph nodes of sarcoidosis patients. Journal of Leukocyte Biology, 105(4), 797-807.

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Multiparametric Immunofluorescence Profiling

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Frozen skin sections were fixed with acetone and blocked with 10% normal goat serum (Vector Laboratories) for 30 minutes. Primary antibodies were incubated overnight at 4°C and amplified with the appropriate Alexa Fluor® 488 (A-488) or 568 (A-568) conjugated secondary antibody for 30 minutes at room temperature. Antibodies used are: IL-32αβγδ (KU32–52, BioLegend), CD3 (SK7, BD Biosciences), CD4 (SK3, BD Biosciences), CD8 (SK1, BD Biosciences), hNKp46 (195314, R&D Systems), CD20 (L27, BD Biosciences), CD14 (M5E2, BioLegend), CD11c (B-ly6, BD Pharmingen), CD303 (AC144, Miltenyi Biotec), and CD163 (5C6-FAT, Acris Antibodies).

Ohmatsu H., Humme D., Gulati N., Gonzalez J., Möbs M., Suárez-Fariñas M., Cardinale I., Mitsui H., Guttman-Yassky E., Sterry W, & Krueger J.G. (2014). Interleukin-32 is progressively expressed in Mycosis Fungoides independent of helper T-cell 2 and helper T-cell 9 polarization. Cancer immunology research, 2(9), 890-900.

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Comprehensive PBMC Phenotypic Analysis

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Phenotypic analysis was performed on PBMCs using panels containing Live/Dead Blue (Life technologies), CD1c (AD5-8E7; Miltenyi), CD3 (SP34-2 and SK7; BD), CD10 (HI10a; Biolegend), CD11c (B-Ly6, BD), CD14 (M5E2, BD), CD16 (3GE, Biolegend), CD19 (HIB19; Biolegend), CD20 (L27, BD), CD34 (561, Biolegend), CD45RA (HI100, Biolegend), CD56 (HCD56, BD), CD62L (SK11, BD), CD66abce (TET2, Miltenyi Biotec), CD80 (L307.4, BD), CD86 (2331; BD); CD123 (7G3; BD), CD133 (7, Biolegend), CD141 (AD5-14H12; Miltenyi), CD146 (P1H12; Biolegend), HLA-DR (TU36, Life technologies), CCR2 (K036C2, Biolegend), CCR4 (L291H4, Biolegend), CCR6 (11A9, BD) and CCR7 (150503, BD) (Panels summarized in S3 Table). Fixation was performed with 1% paraformaldehyde. Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star).

Vangeti S., Strandin T., Liu S., Tauriainen J., Räisänen-Sokolowski A., Cabrera L., Hassinen A., Mäkelä S., Mustonen J., Vaheri A., Vapalahti O., Klingström J, & Smed-Sörensen A. (2021). Monocyte subset redistribution from blood to kidneys in patients with Puumala virus caused hemorrhagic fever with renal syndrome. PLoS Pathogens, 17(3), e1009400.

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Comprehensive Immune Phenotyping Protocol

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Phenotypic analysis was performed on NPA cells and PBMCs using Live/Dead Blue; lineage markers CD3 (SK7; BD), CD19 (HIB19; Biolegend), CD20 (L27, BD), CD45 (HI30; BD), CD56 (HCD56, BD) and CD66abce (TET2, Miltenyi Biotec); HLA-DR (TU36, Life Technologies), CD14 (M5E2, BD), CD16 (3GE, Biolegend), CD11c (B-Ly6, BD), CD1c (AD5-8E7; Miltenyi), CD141 (AD5-14H12; Miltenyi), CD123 (7G3; BD); maturation markers CD83 (HB15e, Biolegend) and CD86 (2331; BD); adhesion marker CD62L (SK11, BD); and migration markers CCR2 (K036C2, Biolegend) and CCR7 (150503: BD); and fixed with 1% paraformaldehyde for flow cytometry. Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences) and analysed using FlowJo software v10 (Tree Star).

Vangeti S., Falck-Jones S., Yu M., Österberg B., Liu S., Asghar M., Sondén K., Paterson C., Whitley P., Albert J., Johansson N., Färnert A, & Smed-Sörensen A. (2023). Human influenza virus infection elicits distinct patterns of monocyte and dendritic cell mobilization in blood and the nasopharynx. eLife, 12, e77345.

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