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Ventana discovery ultra automate

Manufactured by Roche
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Sourced in Switzerland

The Ventana Discovery ULTRA is an automated slide staining system designed for immunohistochemistry (IHC) and in situ hybridization (ISH) applications. It is capable of performing multiple steps in the staining process, including deparaffinization, antigen retrieval, primary antibody incubation, and detection.

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Ventana Discovery Ultra Ventana Discovery Ventana Ultra Discovery Ultra

2 protocols using ventana discovery ultra automate

1

Triple Chromogenic Immunohistochemistry Assay

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The triple chromogenic immunohistochemistry assay was performed using the Ventana Discovery ULTRA automate (Roche Diagnostics, Rotkreuz, Switzerland). All steps were performed automatically with Ventana solutions except if specified otherwise. Dewaxed and rehydrated paraffin sections were pretreated with heat using the CC1 solution for 40 minutes at 95°C. Primary antibodies were applied and revealed sequentially either with a rat Immpress HRP (Ready to use, Vector laboratories Laboratories) or a rabbit UltraMap HRP followed by incubation with a chromogen (ChromoMap DAB, Discovery purple and Discovery Teal). A heat denaturation step was performed after every revelation. The primary antibodies sequence was: rat anti-CD31, rat anti-CD8 and rabbit anti-PanCytokeratin. Sections were counterstained with Harris hematoxyline (J.T. Baker) and permanently mounted with Pertex (Sakura). For immunohistochemical quantification of CD8+ cells and CD31+ cells, 10 × 10 tiled bright-field pictures of FPPE sections were taken at 100 mm magnification. Cell counts were obtained using ImageJ software. All Abs are listed in the Key resource table.
Bruand M., Barras D., Mina M., Ghisoni E., Morotti M., Lanitis E., Fahr N., Desbuisson M., Grimm A., Zhang H., Chong C., Dagher J., Chee S., Tsianou T., Dorier J., Stevenson B.J., Iseli C., Ronet C., Bobisse S., Genolet R., Walton J., Bassani-Sternberg M., Kandalaft L.E., Ren B., McNeish I., Swisher E., Harari A., Delorenzi M., Ciriello G., Irving M., Rusakiewicz S., Foukas P.G., Martinon F., Dangaj Laniti D, & Coukos G. (2021). Cell-autonomous inflammation of BRCA1-deficient ovarian cancers drives both tumor-intrinsic immunoreactivity and immune resistance via STING. Cell reports, 36(3), 109412.
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2

Triple Chromogenic Immunohistochemistry Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The triple chromogenic immunohistochemistry assay was performed using the Ventana Discovery ULTRA automate (Roche Diagnostics, Rotkreuz, Switzerland). All steps were performed automatically with Ventana solutions except if specified otherwise. Dewaxed and rehydrated paraffin sections were pretreated with heat using the CC1 solution for 40 minutes at 95°C. Primary antibodies were applied and revealed sequentially either with a rat Immpress HRP (Ready to use, Vector laboratories Laboratories) or a rabbit UltraMap HRP followed by incubation with a chromogen (ChromoMap DAB, Discovery purple and Discovery Teal). A heat denaturation step was performed after every revelation. The primary antibodies sequence was: rat anti-CD31, rat anti-CD8 and rabbit anti-PanCytokeratin. Sections were counterstained with Harris hematoxyline (J.T. Baker) and permanently mounted with Pertex (Sakura). For immunohistochemical quantification of CD8+ cells and CD31+ cells, 10 × 10 tiled bright-field pictures of FPPE sections were taken at 100 mm magnification. Cell counts were obtained using ImageJ software. All Abs are listed in the Key resource table.
Bruand M., Barras D., Mina M., Ghisoni E., Morotti M., Lanitis E., Fahr N., Desbuisson M., Grimm A., Zhang H., Chong C., Dagher J., Chee S., Tsianou T., Dorier J., Stevenson B.J., Iseli C., Ronet C., Bobisse S., Genolet R., Walton J., Bassani-Sternberg M., Kandalaft L.E., Ren B., McNeish I., Swisher E., Harari A., Delorenzi M., Ciriello G., Irving M., Rusakiewicz S., Foukas P.G., Martinon F., Dangaj Laniti D, & Coukos G. (2021). Cell-autonomous inflammation of BRCA1-deficient ovarian cancers drives both tumor-intrinsic immunoreactivity and immune resistance via STING. Cell reports, 36(3), 109412.
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