Beta amyloid 1 42

Ten acridones were extracted from the stems of Atalantia monophylla, including N-methylatalaphylline, atalaphylline, N-methylatalaphyllinine, atalaphyllinine, N-methylcycloatalaphylline A, citrusinine I, citrusinine II, glycosparvarine, citruscridone, and buxifoliadine C (Figure 1). The isolation and structural elucidation is described elsewhere [45 (link)]. Before use, all acridones were prepared as stock solution in ethanol or DMSO at a concentration of 10 mM. Acetylthiocholine iodide (ATCI), beta amyloid 1-42 (Aβ_1-42), tacrine, trolox, trypsin, fetal bovine serum (FBS), and Dulbecco’s modified Eagle medium nutrient mixture F-12 (DMEM/F12) were purchased from Sigma-Aldrich (SM Chemical supplies Co., Ltd., Bangkok, Thailand), Merck (Merck, Bangkok, Thailand), Gibthai (GT Chemical supplies Co., Ltd., Bangkok, Thailand), and Fluka (SM Chemical supplies Co., Ltd., Bangkok, Thailand). Human neuroblastoma cell line SH-SY5Y cells were purchased from ATCC (A.N.H. Scientific Marketing Co., Ltd., Bangkok, Thailand). AutoDock program version 4.2.6 (the Scripps Research Institute, La Jolla, CA, USA) and BIOVIA Discovery Studio 2017 (BIOVIA, San Diego, CA, USA) were used in the molecular docking study.

Nine coumarins extracted from the root of Toddalia asiatica, including phellopterin, isopimpinellin, toddalolactone, toddaculin, toddacoumaquinone, toddalenone, toddanone, artanin, and fraxinol (Figure 1), were gifted by Chavi Yenjai (Faculty of science, Khon Kaen university, Thailand). The isolation and structural elucidation were explained in a previous report [30 (link),31 (link)]. Acetylthiocholine iodide (ATCI), bovine serum albumin (BSA), beta amyloid 1-42 (Aβ1–42), tacrine, trolox, N-acetyl cysteine (NAC), trypsine, fetal bovine serum (FBS), and Dulbecco’s modified Eagle medium nutrient mixture F-12 (DMEM/F12) were purchased from Sigma-Aldrich (SM Chemical supplies Co., Ltd., Bangkok, Thailand), Merck (Merck, Bangkok, Thailand), Gibthai (GT Chemical supplies Co., Ltd., Bangkok, Thailand), and Fluka (SM Chemical supplies Co., Ltd., Bangkok, Thailand). AutoDock program version 4.2.6 (the Scripps Research Institute, La Jolla, CA, USA) and BIOVIA Discovery Studio 2017 (BIOVIA, San Diego, CA, USA) were used in the molecular docking study.