Pao1 of p aeruginosa

Unless otherwise specified, all materials were used as received. The strain PAO1 of P. aeruginosa was obtained from the ATCC. Non-diseased human bronchial epithelial cells were purchased from the Marsico Lung Institute Tissue Procurement Core and cultured at the air liquid interface as previously described.(4 (link),5 (link)) The surfactant NP40 was a kind gift from Dr. Brian Button at UNC Chapel Hill. The reducing agent TCEP, the DNA degradation enzyme DNase, carboxylated 1 µm polystyrene microspheres, and common laboratory chemicals were purchased from MilliPore Sigma.(25 (link),32 (link)) Hyaluronic acid (ultra-low molecular weight) with a molecular weight of <6 kDa was purchased from LotionCrafters and its characterization has been previously described.(38 (link))

Unless otherwise specified, all materials were used as received. The strain PAO1 of P. aeruginosa was obtained from the ATCC. Non-diseased human bronchial epithelial cells were purchased from the Marsico Lung Institute Tissue Procurement Core and cultured at the air liquid interface as previously described [4 (link), 5 (link)]. The UNC IRB determined that the use of these cells constituted exempt research (23–0186). The surfactant nonyl phenoxypolyethoxylethanol (NP40) was a kind gift from Dr. Brian Button at UNC Chapel Hill. The reducing agent TCEP, the DNA degradation enzyme DNase, carboxylated 1 μm polystyrene microspheres, and common laboratory chemicals were purchased from MilliPore Sigma [25 (link), 32 (link)]. Hyaluronic acid (ultra-low molecular weight) with a molecular weight of <6 kDa was purchased from LotionCrafters and its characterization has been previously described [38 (link)].