Mouse glioma cells (GL261) were kindly provided by Professor Maria da Conceição Pedroso de Lima (Center for Neuroscience and Cell Biology, University of Coimbra, Portugal). This cell line was chosen since it is a very common model for GBM studies, presenting normal features of an astrocytoma (Strong et al., 2018 (link)) and is resistant to TMZ therapy similarly to human GBM (Haddad et al., 2021 (link)). Moreover, xenografts of these cells display histopathological features that closely resemble the human GBM (Yi et al., 2013 (link)). GL261 cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; Gibco) containing 4.5 g/L of glucose, Glutamax, 25 mM HEPES and supplemented with 10% of heat-inactivated (30 min, 56°C) fetal bovine serum (FBS; Biochrome) and 1% penicillin/streptomycin (Gibco) in a humidified incubator at 37°C and 5% CO2.
Fetal bovine serum fbs
Manufactured by Biochrome
Sourced in Germany
Fetal bovine serum (FBS) is a common cell culture supplement derived from the blood of bovine fetuses. It provides essential nutrients, growth factors, and other components that support the growth and maintenance of various cell types in vitro. FBS is a widely used and versatile product in cell culture research and applications.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin p s, by Merck Group (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Ethyl butyrate, by Merck Group (1 mentions)
Sodium octanoate, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Potassium permanganate, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Span 80, by Merck Group (1 mentions)
Tetrazolium dye mtt, by Merck Group (1 mentions)
Copper 2 chloride, by Merck Group (1 mentions)
7 protocols using fetal bovine serum fbs
1
Glioblastoma GL261 Cell Culture Protocol
2
Neonatal Rat Cardiomyocyte Culture Protocol
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Cell culture media, fetal bovine serum (FBS), and horse serum (HS) were purchased from Biochrome (Berlin, Germany); all other chemicals were from Sigma-Aldrich (St Louis, MO). Isolation of neonatal rat cardiomyocytes (NRCM) was performed by enzymatic digestion of neonatal rat hearts as described previously (Hilfiker-Kleiner et al., 2004 (link)). NRCM were cultured in plating medium containing 5% FBS and 10% HS for 24 hr followed by serum-starvation for 48 hr. NRCM were cultured in serum-free medium containing either EMD (10 µM) or the corresponding volume of the solvent DMSO (1 µl/ ml) for additional 24 hr at 37°C or 42°C with 5% CO2.
3
Culturing Prostate Cancer Cell Lines
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The human prostate cancer cell lines, PC3 (ATCC) and C4-2B (a kind gift from Prof. E. Keller, Ann Arbor, MI, USA) were cultured in RPMI 1640 medium (Thermo Fisher Scientific, Schwerte, Germany) containing 10% or 20% fetal bovine serum (FBS; Biochrome, Berlin, Germany) and 1% penicillin/streptomycin (P/S; Sigma-Aldrich, Hamburg, Germany), respectively. Murine prostate cancer cell lines RM1 and TRAMP-C2 (a kind gift from Prof. M. King, Cornell University, NY, USA) were cultivated in DMEM medium (Thermo Fisher Scientific) containing 10% FBS and 1% P/S. Cells were cultured at 37°C in humidified atmosphere (5% CO2). All cells were regularly authenticated and matched with short tandem repeat DNA profiles of the original cancer cell lines.
4
Cytotoxicity Evaluation of Curcumin Formulations
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Standard laboratory grade chemicals, including curcumin, sodium octanoate (commonly called sodium caprylate), and ethyl butyrate were provided by Sigma Chemical Co. (Taufkirchen, Germany). Pluronic surfactant F127 was procured from BASF Inc. (Mount Olive, NJ, USA) DMSO, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), and cisplatin were procured from Sigma-Aldrich Chemical Company (St Louis, MO, USA). Fetal bovine serum (FBS) was procured from Biochrome (Berlin, Germany). Culture mediums, including Dulbecco’s Modified Eagle’s medium (DMEM) and RPMI, and also antibiotic/antimycotic solution were supplied by INOCLON (G. Innovative Biotech Co, Tehran, Iran). Plastic materials were obtained from SPL life Science (SPL, Seoul, Korea). All other chemicals/reagents were of a high quality and used as such.
5
Cytotoxicity Evaluation of 5-FU in MCF-7 Cells
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Urea, absolute ethanol, acetic acid (99.5%), and Span 80 were provided by Merck (Darmstadt, Germany). Sulfuric acid (98%), potassium permanganate, dialysis bag (12 kD cut off), hydrogen peroxide, annexins V- fluorescein isothiocyanate (FITC), Propidium Iodide (PI), 3-(4,5-dimethylthiazol-2-y1)-2,5-dipheny1 tetrazolium bromide (MTT) Powder, and 5-FU were obtained from Sigma Chemical Co. Olive oil was purchased from Niri Organic products (Tehran, Iran). A 1% penicillin/streptomycin solution, phosphate-buffered saline (PBS), RPMI 1640 and Dulbecco’s modified Eagle’s medium (DMEM) as culture media were supplied by INOCLON (G. Innovative Biotech Co, Tehran, Iran). Fetal bovine serum (FBS) was provided by Biochrome (Darmstadt, Germany). MCF-7 human breast cancer was obtained from the Research Institute of Biotechnology (Mashhad, Iran).
6
Cytotoxicity Evaluation of Metal Salts
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Tetrazolium dye (MTT) and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS), Dulbecco's modified Eagle medium (DMEM), and 1% penicillin–streptomycin solution were procured from INOCLON (Tehran, Iran). Fetal bovine serum (FBS) was purchased from Biochrome (Berlin, Germany). Ferric nitrate (Fe (NO3)3. 9H2O, ≥ 98%), zinc nitrate (Zn(NO3)2·6H2O, 98%), and copper (II) chloride (CuCl2·2H2O, ≥ 99.0%) salts were purchased from Sigma-Aldrich Company. All the steps were performed under sterile conditions. Deionized water was utilized in all stages. A549 human lung adenocarcinoma cancer cells and murine macrophage cell line (J774-A1) were obtained from the Pasteur Institute of Iran's (Iran) cellular bank. Cells were cultivated in DMEM medium supplemented with 10% FBS, 1% antibiotic mixture (penicillin/streptomycin), and maintained at humidified atmosphere under standard conditions (37 °C, 5% CO2).
7
Cell Culture Conditions for HEK-293T and Nalm-6
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HEK-293T, Lenti-X-293T, and β2Mk/oCD47high 293T cells were grown in DMEM (Biowest) supplemented with 10% fetal bovine serum (FBS; Biochrome, Berlin, Germany) and 2 mM L-glutamine (Sigma-Aldrich). Nalm-6 cells were cultured in RPMI 1640 (Biowest) supplemented with 10% FBS and 2 mM L-glutamine.
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