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P44 p42 mapk t202 y204 rabbit 4370s

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P44/p42 MAPK (T202/Y204) rabbit 4370S is a laboratory reagent used for the detection and analysis of phosphorylated p44/p42 mitogen-activated protein kinase (MAPK) in biological samples. It is a rabbit polyclonal antibody that specifically recognizes the phosphorylated threonine 202 and tyrosine 204 residues of human p44/p42 MAPK.

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Lab products found in correlation

Anti syk, by Santa Cruz Biotechnology (2 mentions) Anti p erk, by Cell Signaling Technology (2 mentions)

2 protocols using p44 p42 mapk t202 y204 rabbit 4370s

1

Erk Phosphorylation Assay in DT40 Cells

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For the analysis of Erk phosphorylation, DT40 Syk-AQL-EGFP cells were treated with or without goat-anti-mouse IgM (10 (μg/mL) for the indicated periods of time at 37 °C and then lysed in buffer containing 25% sucrose, 2.5% SDS, 25 mM Tris/2.5 mM EDTA, 2.5 mg pyronin Y, and 2% 2-mercaptoethanol. The DNA in lysates was sheared by passing through a 26 G × 1/2 in needle. Proteins in the lysate were separated by SDS-PAGE, transferred to polyvinylidene difluoride membrane, and analyzed by Western blotting with anti-pERK (Cell Signaling p44/p42 MAPK (T202/Y204) rabbit 4370S), and anti-Syk (Santa Cruz N-19 rabbit) antibodies. The results of these assays were used for parameter screening and will be referenced in the results section.
McGee R.L., Krisenko M.O., Geahlen R.L., Rundell A.E, & Buzzard G.T. (2015). A Computational Study of the Effects of Syk Activity on B Cell Receptor Signaling Dynamics. Processes (Basel, Switzerland), 3(1), 75-97.
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2

Erk Phosphorylation Assay in DT40 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the analysis of Erk phosphorylation, DT40 Syk-AQL-EGFP cells were treated with or without goat-anti-mouse IgM (10 (μg/mL) for the indicated periods of time at 37 °C and then lysed in buffer containing 25% sucrose, 2.5% SDS, 25 mM Tris/2.5 mM EDTA, 2.5 mg pyronin Y, and 2% 2-mercaptoethanol. The DNA in lysates was sheared by passing through a 26 G × 1/2 in needle. Proteins in the lysate were separated by SDS-PAGE, transferred to polyvinylidene difluoride membrane, and analyzed by Western blotting with anti-pERK (Cell Signaling p44/p42 MAPK (T202/Y204) rabbit 4370S), and anti-Syk (Santa Cruz N-19 rabbit) antibodies. The results of these assays were used for parameter screening and will be referenced in the results section.
McGee R.L., Krisenko M.O., Geahlen R.L., Rundell A.E, & Buzzard G.T. (2015). A Computational Study of the Effects of Syk Activity on B Cell Receptor Signaling Dynamics. Processes (Basel, Switzerland), 3(1), 75-97.
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