Rabbit anti β actin polyclonal antibody

The rabbit polyclonal anti-SPARC antibody (15274-1-AP) was purchased from Proteintech. The mouse monoclonal anti-human SPARC (clone AON-5031, sc-73472), the rabbit polyclonal anti-human cath-D antibody (H-75, sc-10725), and the mouse monoclonal anti-human cath-D (clone C-5, sc-377124) antibodies were purchased from Santa Cruz Biotechnology. The mouse monoclonal anti-human cath-D antibody (clone 49, #610801) was purchased from BD Transduction Laboratories^TM, and the goat polyclonal anti-mouse cath-D (AF1029) from R&D Systems. The anti-human cath-D antibodies M1G8 and D7E3 were previously described 19 (link). The mouse monoclonal anti-tubulin antibody (clone 236-10501, #A11126) was from Thermo Fisher Scientific, the mouse monoclonal anti-Myc (clone 9B11) from Ozyme, and the rabbit polyclonal anti-β actin antibody (#A2066) from Sigma-Aldrich. The horse anti-mouse immunoglobulin G (IgG)-horseradish peroxidase (#7076), and goat anti-rabbit IgG-HRP (#7074S) secondary antibodies were from Cell Signaling Technology. The donkey anti-goat HRP conjugated antibody (FT-1I7890) was from Interchim. The Alexa Fluor 488-conjugated anti-rabbit IgG (#Ab150077) was purchased from Abcam, and the Cy3-conjugated anti-mouse IgG (#SA00009.1) from Proteintech. Hoechst 33342 (#FP-BB1340) was from Interchim FluoProbes.

For western blotting, equal amount of proteins were initially boiled in Tris-lysis buffer and electrophoresed on a 10% SDS-polyacrylamide gel. Proteins were transferred onto nitrocellulose membrane (VWR) and followed by blocking in 5% milk (in 0.1% PBS-Tween-20). Blots were probed for lactate dehydrogenase A (LDHA) (C4B5. Cell Signaling Technology (Beverly, MA) was visualized with SuperSignal (ThermoFisher Scientific), using the appropriate secondary antibody. Rabbit polyclonal anti-β-actin antibody (Sigma, St Louis, MO) was used as a loading control.

CFZ was purchased from Selleck Chemicals (Houston, TX) and was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 1 mM, and aliquots were stored at −80°C. Stock solutions were diluted to the desired final concentrations with growth medium just before use. CHX and actinoimycin D were purchased from Sigma Chemical Co. (St. Louis, MO). Human recombinant TRAIL was purchased from PeproTech, Inc. (Rocky Hill, NJ). Rabbit polyclonal anti-DR5 antibody was purchased from ProSci, Inc. (Poway, CA). Mouse monoclonal anti-DR4 antibody (B-N28) was purchased from Diaclone (Stamford, CT). Mouse monoclonal anti-caspase-3 was purchased from Imgenex (San Diego, CA). Rabbit anti-caspase-8 and anti-PARP antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA). Mouse monoclonal anti-FLIP antibody (NF6) was purchased from Alexis Biochemicals (San Diego, CA). Rabbit polyclonal Mcl-1 and Bcl-X_L and mouse monoclonal Bcl-2 antibody were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Rabbit polyclonal anti-β-actin antibody was purchased from Sigma Chemical Co.