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Micromax

Manufactured by Teledyne
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Sourced in United States, France

The Micromax is a versatile lab equipment designed for precision measurements and analysis. It features a high-resolution display and intuitive controls for accurate data collection and processing. The core function of the Micromax is to provide reliable and consistent performance in various laboratory settings.

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Lab products found in correlation

Metamorph software, by Teledyne (3 mentions) Hoechst 33342, by Merck Group (3 mentions) Dm6000, by Teledyne (2 mentions) E600 upright microscope, by Nikon (2 mentions) Metamorph imaging software, by Universal Imaging (2 mentions) Dm6000, by Leica (1 mentions) Metaview software, by Universal Imaging (1 mentions) Axiovert 100 tv, by Zeiss (1 mentions) Metafluor imaging software, by Universal Imaging (1 mentions) Axio observer, by Zeiss (1 mentions) Metamorph 7, by Molecular Devices (1 mentions) Mitotracker red cm h2xros, by Thermo Fisher Scientific (1 mentions) Imt 2 inverted microscope, by Olympus (1 mentions) Metafluor software, by Universal Imaging (1 mentions) Axiovert 200, by Zeiss (1 mentions) Hoechst 33342, by Leica (1 mentions) Tcs sp8 x confocal laser scanning microscope, by Leica (1 mentions) Axiovert 200 inverted microscope, by Zeiss (1 mentions) Polylysine, by Merck Group (1 mentions) Metamorph software, by Molecular Devices (1 mentions)

17 protocols using micromax

1

Quantitative Analysis of Synaptic Clusters

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Image acquisition was performed using a 63× objective (NA 1.32) on a Leica DM6000 upright epifluorescence microscope with a 12-bit cooled CCD camera (Micromax, Roper Scientific) run by MetaMorph software (Roper Scientific). Quantification was performed using MetaMorph software. Image exposure time was determined on bright cells to obtain the best fluorescence-to-noise ratio and avoid pixel saturation. All images from a given culture were then acquired with the same exposure time and acquisition parameters. For each image, several dendritic regions of interest were manually chosen, and a user-defined intensity threshold was applied to select clusters and avoid their coalescence. For quantification of gephyrin or GABAAR α2 synaptic clusters, gephyrin or receptor clusters comprising at least 3 pixels and colocalized on at least 1 pixel with VGAT clusters were considered. The integrated fluorescence intensities of clusters were measured.
Battaglia S., Renner M., Russeau M., Côme E., Tyagarajan S.K, & Lévi S. (2018). Activity-Dependent Inhibitory Synapse Scaling Is Determined by Gephyrin Phosphorylation and Subsequent Regulation of GABAA Receptor Diffusion. eNeuro, 5(1), ENEURO.0203-17.2017.
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2

Live Cell Imaging of EGFP-MexB Expression

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The cells suspended in the PBS buffer were imaged in a micro-chamber using dark-field optical microscopy and epi-fluorescence microscopy (Nikon, E-400) equipped with a CCD camera (Micromax, Roper Scientific). The design and construction of the micro-chamber and dark-field optical microscopy for imaging of single live cells were fully described in our previous studies.33 (link), 35 (link), 38 (link), 46 In this study, the fluorescence filter cube (Chroma Tech) containing a band-pass excitation filter (455 ± 30 nm), band-pass emission filter (525 ± 30 nm) and a dichroic mirror (500 nm), was used for fluorescence imaging of expression of EGFP-MexB in single live cells. The dark-field optical microscope is equipped with a dark-field condenser (Oil 1.43-1.20, Nikon) and a 100x objective (Nikon Plan fluor 100× oil, iris, SL. N.A. 0.5-1.3, W.D. 0.20 mm).
Ding F., Lee K.J., Vahedi-Faridi A., Yoneyama H., Osgood C.J, & Xu X.H. (2014). Design and Study of Efflux Function of EGFP Fused MexAB-OprM Membrane Transporter in Pseudomonas aeruginosa Using Fluorescence Spectroscopy. The Analyst, 139(12), 3088-3096.
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3

Quantifying Neuronal Spine and Synaptic Protein Dynamics

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Image acquisition was performed using a ×100 objective (NA 1.40) on a Leica (Nussloch, Germany) DM6000 upright epifluorescence microscope with a 12-bit cooled CCD camera (Micromax, Roper Scientific) run by MetaMorph software (Roper Scientific, Evry, France). Quantification was performed using MetaMorph software (Roper Scientific). Image folders were randomized before analysis. For morphological spine analysis exposure time was adjusted for each eGFP image to obtain best fluorescence to noise ratio and to avoid pixel saturation. For each neuron a well-focused dendrite was chosen, spine heads were manually delimited and their area were quantified. To assess KCC2-Flag clusters, exposure time was fixed at a non-saturating level and kept unchanged between cells and conditions. For cluster analysis, images were first flatten background filtered (kernel size, 3 × 3 × 2) to enhance cluster outlines, and a user defined intensity threshold was applied to select clusters and avoid their coalescence. Clusters were outlined and the corresponding regions were transferred onto raw images to determine the mean KCC2–Flag cluster number, area and fluorescence intensity. The dendritic surface area of the region of interest was measured to determine the number of clusters per 10 µm2. For each culture, we analyzed ~10 cells per experimental condition and ~100 clusters or ~15 spines per cell.
Heubl M., Zhang J., Pressey J.C., Al Awabdh S., Renner M., Gomez-Castro F., Moutkine I., Eugène E., Russeau M., Kahle K.T., Poncer J.C, & Lévi S. (2017). GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl−-sensitive WNK1 kinase. Nature Communications, 8, 1776.
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4

Quantitative analysis of synaptic clusters

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Image acquisition was performed using a 63× objective (NA 1.32) on a Leica (Nussloch, Germany) DM6000 upright epifluorescence microscope with a 12-bit cooled CCD camera (Micromax, Roper Scientific) run by MetaMorph software (Roper Scientific, Evry, France). Image exposure time was determined on bright cells to obtain best fluorescence to noise ratio and to avoid pixel saturation. All images from a given culture were then acquired with the same exposure time and acquisition parameters. For cluster colocalization analysis, quantification was performed using MetaMorph software (Roper Scientific). For each image, several dendritic regions of interest were manually chosen and a user-defined intensity threshold was applied to select clusters and avoid their coalescence. For quantification of gephyrin or GABAAR synaptic clusters, gephyrin or receptor clusters comprising at least 3 pixels and colocalized on at least 1 pixel with VGAT clusters were considered. The number of clusters, the surface area and the integrated fluorescence intensities of clusters were measured. For surface expression analysis, quantification was performed using ImageJ (National Institutes of Health and LOCI, University of Wisconsin). Several dendritic regions of interest were manually chosen and mean average intensity per pixel was measured.
Merlaud Z., Marques X., Russeau M., Saade U., Tostain M., Moutkine I., Gielen M., Corringer P.J, & Lévi S. (2022). Conformational state-dependent regulation of GABAA receptor diffusion and subsynaptic domains. iScience, 25(11), 105467.
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5

Immunofluorescence Imaging of Fixed Cells

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Fixed cells were processed for immunofluorescence staining as previously described [17 (link)], and were imaged with an epifluorescence microscope (Leica DMRX, HCX PL APO 63x/1.32NA or 100x/1.35NA oil immersion lenses) equipped with the appropriate excitation and emission filters. Images were acquired with a linear CCD camera (Micromax; Princeton instruments) and Metaview software (Universal Imaging). All confocal microscopy experiments with living cells and fixed samples were performed with a Zeiss LSM 510 Meta laser scanning confocal microscope (Carl Zeiss, Jena, Germany) using objectives and acquisition settings specified below. Living cells were maintained on the microscope stage at 37°C in a 5% CO2 atmosphere using an air-stream incubator and a heating stage insert (Pecon GmbH). Images were analyzed with custom ImageJ plugins (NIH) as described below.
Hadzic E., Catillon M., Halavatyi A., Medves S., Van Troys M., Moes M., Baird M.A., Davidson M.W., Schaffner-Reckinger E., Ampe C, & Friederich E. (2015). Delineating the Tes Interaction Site in Zyxin and Studying Cellular Effects of Its Disruption. PLoS ONE, 10(10), e0140511.
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6

Sperm Incorporation Dynamics in Sea Urchin Eggs

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Sea urchins (Paracentrotus lividus) were captured in the Gulf of Naples during the breeding season (November to May) and maintained in circulating seawater (16 °C). Spawning in female animals was induced by intracoelomic injection of 0.5 M KCl. Eggs were then collected in filtered seawater, and fertilization was performed with freshly diluted sperm at the final spermatozoa concentration of 1.8 × 106 spermatozoa/ml. To count egg-incorporated spermatozoa, sperm nuclei were pre-stained with Hoechst 33,342 (Sigma-Aldrich) at a final concentration of 5 µM in 1 mL natural seawater (NSW) for 10–15 s prior to fertilization. Sperm incorporation into the egg was observed 5–10 min after insemination using a CCD camera (MicroMax, Princeton Instruments, Inc) mounted on a Zeiss Axiovert 200 microscope with a Plan-Neofluar 40×/0.75 objective and a UV laser.
Limatola N., Vasilev F., Santella L, & Chun J.T. (2019). Nicotine Induces Polyspermy in Sea Urchin Eggs through a Non-Cholinergic Pathway Modulating Actin Dynamics. Cells, 9(1), 63.
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7

Microscopic Quantification of Microvessels

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Under microscopy, localization of labeled microvessels was performed with a Nikon E600 upright microscope with a ×20 objective lens. The microscope was coupled to cooled charge‐coupled device camera (Micromax; Princeton Instruments Inc, Trenton, NJ). Five nearby 1‐mm2 images were taken from each of 3 sections in the frontal cortex of each brain or in the GSN‐Ms, and the mean MVD within these images was taken to represent cortical or skeletal muscle MVD in that animal. All acquired images from individual sections were analyzed for number of microvessels using MetaMorph Imaging software (Universal Imaging Co, Downingtown, PA) or Nikon Elements software.41
Frisbee S.J., Singh S.S., Jackson D.N., Lemaster K.A., Milde S.A., Shoemaker J.K, & Frisbee J.C. (2018). Beneficial Pleiotropic Antidepressive Effects of Cardiovascular Disease Risk Factor Interventions in the Metabolic Syndrome. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(7), e008185.
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8

Mitochondrial Membrane Potential Imaging

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Muscle fibers were isolated from FDB muscle and mitochondrial membrane potential measured by epifluorescence microscopy on the basis of the accumulation of TMRM fluorescence, as previously described (Lo Verso et al., 2014 (link)). Fibers were considered as depolarizing when they lost more than 10% of the initial value of TMRM fluorescence. Imaging was performed with a Zeiss Axiovert 100 TV inverted microscope equipped with a 12-bit digital cooled charge-coupled device camera (Micromax, Princeton Instruments). The results were analyzed with MetaFluor imaging software (Universal Imaging).
Mansueto G., Armani A., Viscomi C., D’Orsi L., De Cegli R., Polishchuk E.V., Lamperti C., Di Meo I., Romanello V., Marchet S., Saha P.K., Zong H., Blaauw B., Solagna F., Tezze C., Grumati P., Bonaldo P., Pessin J.E., Zeviani M., Sandri M, & Ballabio A. (2017). Transcription Factor EB Controls Metabolic Flexibility during Exercise. Cell Metabolism, 25(1), 182-196.
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9

Organoid Live Imaging of Mucus Release

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Organoids (Wt, Cdk8Lox/Lox, Cdk19−/−and Cdk8Lox/Lox/Cdk19−/−) were treated with 600 nM 4‐hydroxytamoxifen during 7 days. Once recombination of the LoxP sites flanking Cdk8 exon 2 was obtained, images were taken every 4 h during 7 days on an inverted microscope (Axio Observer, Zeiss) equipped with a heated chamber allowing constant temperature (37°C) and CO2 flow (5% CO2). CCD camera (Princeton Instruments (Micromax), pixel = 6.7 μm), with 10x/0.3 DRY PH1objective, correction ECPLAN Neofluar, 5.2 mm working distance. Acquisition software was MetaMorph 7.8 (Molecular Devices, LLC). Images were analysed using Image J software to calculate the time for release of mucus in the lumen of the organoid (observed as a dark staining in the centre of the organoid).
Prieto S., Dubra G., Camasses A., Aznar A.B., Begon‐Pescia C., Simboeck E., Pirot N., Gerbe F., Angevin L., Jay P., Krasinska L, & Fisher D. (2022). CDK8 and CDK19 act redundantly to control the CFTR pathway in the intestinal epithelium. EMBO Reports, 24(2), e54261.
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10

Quantifying Cortical Microvessel Density

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Following removal of the MCAs from the Circle of Willis on the base of the brain, the brain was placed within Tissue-Tek OCT compound and frozen. Brains were then sliced into 5 μm cross sections and where then stained using the established approach developed by Munzenmaier and Greene (30 (link)) using primary anti-CD-31 antibody. Under microscopy, localization of labeled microvessels was performed with a Nikon E600 upright microscope with a 20x objective lens. The microscope was coupled to cooled CCD camera (Micromax; Princeton Instruments Inc, Trenton, NJ). Five nearby 1 mm2 images were taken from each of three sections in the frontal cortex of each brain, and the mean microvessel density within these 15 images was taken to represent cortical MVD in that animal. All acquired images from individual sections were analyzed for number of microvessels using MetaMorph Imaging software (Universal Imaging Co., Downingtown, PA).
Chantler P.D., Shrader C.D., Tabone L.E., d’Audiffret A.C., Huseynova K., Brooks S.D., Branyan K.W., Grogg K.A, & Frisbee J.C. (2015). CEREBRAL CORTICAL MICROVASCULAR RAREFACTION IN METABOLIC SYNDROME IS DEPENDENT ON INSULIN RESISTANCE AND LOSS OF NITRIC OXIDE BIOAVAILABILITY. Microcirculation (New York, N.Y. : 1994), 22(6), 435-445.
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