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Murashige and skoog

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Murashige and Skoog is a plant growth medium used for the in vitro culture of plant cells, tissues, and organs. It provides the essential nutrients required for plant growth and development in a controlled laboratory environment.

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Lab products found in correlation

Gellan gum powder, by MP Biomedicals (2 mentions)

2 protocols using murashige and skoog

1

Tobacco Plant Transgenesis and Cultivation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The plant transgenesis and cultivation was carried out at the artificial climate station Biotron N2-2.9 (branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Russia). Tobacco plants were propagated on Murashige and Skoog (MS) medium supplemented with 30 g/l sucrose and 0.8 w/v agar (Panreac, Spain). In vitro cultures were incubated at 24±1°C with 12-16-day photoperiod, with mixed cool white and red light (Cool White and Grolux fluorescent lamps) at light intensity 40 μmol / sec*m2 (link). After root development, plantlets were transferred to 9 cm pots with sterilized soil (1:3 w/w mixture of sand and peat). Potted plants were placed in the greenhouse at 22±2°C under neutral day conditions (12h light / 12h dark; 150 μmol m-2s-1) and 75% relative humidity. For time-lapse imaging of germinating seeds (Supplementary Video 2), seeds were sterilized in sodium hypochlorite (25%/15 min) and then propagated on Murashige and Skoog (MS) medium supplemented with 30 g/l sucrose and 0.3 w/v Gellan Gum Powder (MP Biomedicals,LLC). The same medium was used for luminescence imaging of roots (panel iv in Figure 2, Supplementary Videos 3 and 4).
Mitiouchkina T., Mishin A.S., Somermeyer L.G., Markina N.M., Chepurnyh T.V., Guglya E.B., Karataeva T.A., Palkina K.A., Shakhova E.S., Fakhranurova L.I., Chekova S.V., Tsarkova A.S., Golubev Y.V., Negrebetsky V.V., Dolgushin S.A., Shalaev P.V., Shlykov D., Melnik O.A., Shipunova V.O., Deyev S.M., Bubyrev A.I., Pushin A.S., Choob V.V., Dolgov S.V., Kondrashov F.A., Yampolsky I.V, & Sarkisyan K.S. (2020). Plants with genetically encoded autoluminescence. Nature biotechnology, 38(8), 944-946.
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2

Tobacco Plant Transgenesis and Cultivation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The plant transgenesis and cultivation was carried out at the artificial climate station Biotron N2-2.9 (branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Russia). Tobacco plants were propagated on Murashige and Skoog (MS) medium supplemented with 30 g/l sucrose and 0.8 w/v agar (Panreac, Spain). In vitro cultures were incubated at 24±1°C with 12-16-day photoperiod, with mixed cool white and red light (Cool White and Grolux fluorescent lamps) at light intensity 40 μmol / sec*m2 (link). After root development, plantlets were transferred to 9 cm pots with sterilized soil (1:3 w/w mixture of sand and peat). Potted plants were placed in the greenhouse at 22±2°C under neutral day conditions (12h light / 12h dark; 150 μmol m-2s-1) and 75% relative humidity. For time-lapse imaging of germinating seeds (Supplementary Video 2), seeds were sterilized in sodium hypochlorite (25%/15 min) and then propagated on Murashige and Skoog (MS) medium supplemented with 30 g/l sucrose and 0.3 w/v Gellan Gum Powder (MP Biomedicals,LLC). The same medium was used for luminescence imaging of roots (panel iv in Figure 2, Supplementary Videos 3 and 4).
Mitiouchkina T., Mishin A.S., Somermeyer L.G., Markina N.M., Chepurnyh T.V., Guglya E.B., Karataeva T.A., Palkina K.A., Shakhova E.S., Fakhranurova L.I., Chekova S.V., Tsarkova A.S., Golubev Y.V., Negrebetsky V.V., Dolgushin S.A., Shalaev P.V., Shlykov D., Melnik O.A., Shipunova V.O., Deyev S.M., Bubyrev A.I., Pushin A.S., Choob V.V., Dolgov S.V., Kondrashov F.A., Yampolsky I.V, & Sarkisyan K.S. (2020). Plants with genetically encoded autoluminescence. Nature biotechnology, 38(8), 944-946.
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