Echocardiography assessed cardiac function noninvasively (software: ML750 Power Lab/4sp AD Instruments GE-Vingmed Ultrasound, USA); the animals underwent mild anesthesia with 5% isoflurane and were evaluated by an echo device equipped with a 14-18 MHz heart probe. Echocardiographic parameters were obtained based on the main axis of the heart. Heart failure with an ejection fraction < 55% is clear. Calculation of cardiac output was estimated as (end − diastolic volume–end − systolic volume) × heart rate (ml/min) [3 (link)] (Table 1 ).
Ml750 power lab 4sp
Manufactured by ADInstruments
Sourced in United States, Australia
The ML750 PowerLab/4sp is a data acquisition and analysis system designed for educational and research applications. It provides four analog input channels for recording various physiological signals. The device features built-in signal conditioning capabilities and can be used with a variety of sensors and transducers.
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Lab products found in correlation
7 protocols using ml750 power lab 4sp
1
Noninvasive Cardiac Function Assessment
2
Surgical Induction of Myocardial Infarction
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Myocardial infarction was created by permanent ligation of the left anterior descending coronary artery as described previously (Sun et al., 2001[28 (link)]). Briefly, after intubation, left thoracotomy and pericardiotomy, 6-0 silk suture was placed around the left anterior descending coronary artery localized in 2 mm below the left atrium. The chest was closed and lung reinflated using positive end expiratory pressure. A computerized data acquisition system (ML750 Power Lab/4sp, AD Instruments) was used for monitoring ECG. ST-segment elevation and Q wave inversion were indicators of successful operation. Respiratory functions were preserved through use of a ventilator (Small Animal Ventilator, Model 683, Harvard Apparatus, 15 ml/kg stroke volume and 60-70 breaths/ min) and body temperature was maintained with an incubator that was fixed to a laboratory bench (Ranjbar et al., 2015[25 ]).
3
Rat Myocardial Infarction Model
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Rats were administered heparin (200 IU/kg, IV), and then, the heart of each was exposed through a left thoracotomy between the fourth and fifth ribs (1.5 cm in diameter), and an incision was made into the pericardium. The ligation of the left anterior descending (LAD) coronary artery (close to its origin) was performed using 6-0 silk suture. A standard limb lead-II electrocardiogram (ECG) was continuously monitored and recorded throughout the experiment, using a computerized data acquisition system (ML750 Power Lab/4sp, AD Instruments). Successful ligation of the LAD was confirmed by ST elevation and increase in R-wave amplitude in ECG. Muscle and skin incisions were closed with separate purse-string silk sutures (size 4-0), and the lungs were fully expanded. In transient ischemia group, the ischemic myocardium was reperfused by loosening the ligature after 30 min and in permanent ischemia group, the permanent ligation of the left anterior descending coronary artery is performed Body temperature was measured by rectal thermometer and maintained at 37±1°C (13 (link), 14 (link)).
4
Cardiac Ischemia-Reperfusion Injury in Rats
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The rat model cardiac IR injury was performed by ligation of the left anterior descending coronary artery (LAD) for 30 min followed by 24 h reperfusion as previously described [47 (link)–49 ]. Briefly, after anesthesia (thiopental sodium; 60 mg/kg i.p.), the trachea was intubated and connected to a ventilator (Harvard rodent ventilator model 683, Holliston, MA, USA) and the heart was exposed through a left thoracotomy and pericardium incision. Then, the proline 0–6 polypropylene suture was passed approximately 1–2 mm distal from LAD artery origin and after the condition became stable, ischemia was induced for 30 min by ligation of LAD. Following LAD artery occlusion, the pale of the heart, ST elevation and electrocardiographic changes (ML750 PowerLab/4sp ADInstruments) confirmed successful ligation and myocardial ischemia. Rats in the sham group underwent the same surgical procedure without LAD ligation.
5
Vasa Deferentia Contractility Measurement
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Vasa deferentia segments were mounted vertically on perspex tissue holders so that contractions of the longitudinal muscle could be measured. The tissue holders were placed in separate 10-mL water-jacketed standard organ baths filled with Krebs–Henseleit solution maintained at 37°C and supplied with carbogen (95% oxygen; 5% CO2) gas. Each tissue holder incorporated two platinum electrodes, which were connected to a Grass S88 stimulator (Grass Instruments, MA, USA). The epididymal end of the tissue was tied off and fastened to the tissue holders, and a needle was used to thread cotton ligature through the lumen and tissue wall at the prostatic end of the tissue, which was then attached to a FT03 force displacement transducer (Grass instruments, MA, USA), connected to a ML118 QUAD Bridge (ADInstruments, Australia). The signal was digitized and sent to a personal computer using a ML750 PowerLab/4SP (ADInstruments, Castle Hill, Australia), and data was recorded using LabChart software (version: Chart5 for Windows). The initial tension of the tissues was set at 1.0 g, and tissues were allowed to equilibrate for 1 h under electrical field stimulation (EFS, parameters: pulse duration = 0.5 ms; voltage = 60 V; frequency = 0.01 Hz) prior to experimentation.
6
Hemodynamic Monitoring in Animals
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Hemodynamics were continuously monitored using subcutaneous stainless-steel electrodes that were connected via a cable to a PowerLab monitoring system (ML750 PowerLab/4sp with MLT380 Reusable BP Transducer; AD Instruments, CO Springs, CO). Heart rate, mean arterial pressure (MAP), and the rate pressure product (RPP, the product of heart rate and systolic pressure) were recorded. The RPP was calculated to evaluate the myocardial oxygen demand of the animals [21 (link)].
7
Myocardial Infarction Surgical Procedure
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Myocardial infarction (MI) was performed in accordance with a previous method from our laboratory. Myocardial infarction was induced by permanent ligation of the left anterior descending coronary artery as described previously (17 (link)). Briefly, after intubation, left thoracotomy and pericardio-tomy, 6-0 silk suture was placed around the left anterior descending coronary artery localized 2 mm below the left atrium. The chest was closed and lung reinflated using positive end expiratory pressure. A standard limb lead-II electrocardiogram (ECG) was continuously monitored and recorded throughout the experiment, using a computerized data acqui-sition system (ML750 Power Lab/4sp, AD Instru-ments). Change of ST-segment (elevation) Premature Ventricular Contraction (PVC), Ventricular Tachycardia (V Tach) and ventricular fibrillation were indicators of a successful operation and heart failure confirmation (Figure 1 ). Respiratory functions were preserved through the use of a ventilator (Small Animal Ventilator, Model 683, Harvard Apparatus, 15 ml/kg stroke volume and 60–70 breaths/min) and body temperature was maintained with an incubator that was fixed to a laboratory bench. The sham group underwent the same procedures except that myocardial ischemia was not induced. The chest was closed with a silk suture.
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